In vitro antiviral activity of peptide-rich extracts from seven Nigerian plants against three non-polio enterovirus species C serotypes
Abstract Background As frequent viral outbreaks continue to pose threat to public health, the unavailability of antiviral drugs and challenges associated with vaccine development underscore the need for antiviral drugs discovery in emergent moments (endemic or pandemic). Plants in response to microb...
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| Format: | Article |
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BMC
2021-08-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-021-01628-7 |
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| author | Omonike O. Ogbole Toluwanimi E. Akinleye Abraham O. Nkumah Aminat O. Awogun Alfred F. Attah Moses O. Adewumi Adekunle J. Adeniji |
| author_facet | Omonike O. Ogbole Toluwanimi E. Akinleye Abraham O. Nkumah Aminat O. Awogun Alfred F. Attah Moses O. Adewumi Adekunle J. Adeniji |
| author_sort | Omonike O. Ogbole |
| collection | DOAJ |
| description | Abstract Background As frequent viral outbreaks continue to pose threat to public health, the unavailability of antiviral drugs and challenges associated with vaccine development underscore the need for antiviral drugs discovery in emergent moments (endemic or pandemic). Plants in response to microbial and pest attacks are able to produce defence molecules such as antimicrobial peptides as components of their innate immunity, which can be explored for viral therapeutics. Methods In this study, partially purified peptide-rich fraction (P-PPf) were obtained from aqueous extracts of seven plants by reverse-phase solid-phase extraction and cysteine-rich peptides detected by a modified TLC method. The peptide-enriched fractions and the aqueous (crude polar) were screened for antiviral effect against three non-polio enterovirus species C members using cytopathic effect reduction assay. Results In this study, peptide fraction obtained from Euphorbia hirta leaf showed most potent antiviral effect against Coxsackievirus A13, Coxsackievirus A20, and Enterovirus C99 (EV-C99) with IC50 < 2.0 µg/mL and selective index ≥ 81. EV-C99 was susceptible to all partially purified peptide fractions except Allamanda blanchetii leaf. Conclusion These findings establish the antiviral potentials of plants antimicrobial peptides and provides evidence for the anti-infective use of E. hirta in ethnomedicine. This study provides basis for further scientific investigation geared towards the isolation, characterization and mechanistic pharmacological study of the detected cysteine-rich peptides. |
| first_indexed | 2024-12-19T15:58:17Z |
| format | Article |
| id | doaj.art-1a15a9d0339c4f8d95594ef696573f54 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-19T15:58:17Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-1a15a9d0339c4f8d95594ef696573f542022-12-21T20:14:59ZengBMCVirology Journal1743-422X2021-08-011811710.1186/s12985-021-01628-7In vitro antiviral activity of peptide-rich extracts from seven Nigerian plants against three non-polio enterovirus species C serotypesOmonike O. Ogbole0Toluwanimi E. Akinleye1Abraham O. Nkumah2Aminat O. Awogun3Alfred F. Attah4Moses O. Adewumi5Adekunle J. Adeniji6Department of Pharmacognosy, Faculty of Pharmacy, University of IbadanDepartment of Pharmacognosy, Faculty of Pharmacy, University of IbadanDepartment of Pharmacognosy, Faculty of Pharmacy, University of IbadanDepartment of Pharmacognosy, Faculty of Pharmacy, University of IbadanDepartment of Pharmacognosy and Drug Development, Faculty of Pharmaceutical Sciences, University of IlorinDepartment of Virology, College of Medicine, University of IbadanDepartment of Virology, College of Medicine, University of IbadanAbstract Background As frequent viral outbreaks continue to pose threat to public health, the unavailability of antiviral drugs and challenges associated with vaccine development underscore the need for antiviral drugs discovery in emergent moments (endemic or pandemic). Plants in response to microbial and pest attacks are able to produce defence molecules such as antimicrobial peptides as components of their innate immunity, which can be explored for viral therapeutics. Methods In this study, partially purified peptide-rich fraction (P-PPf) were obtained from aqueous extracts of seven plants by reverse-phase solid-phase extraction and cysteine-rich peptides detected by a modified TLC method. The peptide-enriched fractions and the aqueous (crude polar) were screened for antiviral effect against three non-polio enterovirus species C members using cytopathic effect reduction assay. Results In this study, peptide fraction obtained from Euphorbia hirta leaf showed most potent antiviral effect against Coxsackievirus A13, Coxsackievirus A20, and Enterovirus C99 (EV-C99) with IC50 < 2.0 µg/mL and selective index ≥ 81. EV-C99 was susceptible to all partially purified peptide fractions except Allamanda blanchetii leaf. Conclusion These findings establish the antiviral potentials of plants antimicrobial peptides and provides evidence for the anti-infective use of E. hirta in ethnomedicine. This study provides basis for further scientific investigation geared towards the isolation, characterization and mechanistic pharmacological study of the detected cysteine-rich peptides.https://doi.org/10.1186/s12985-021-01628-7AntiviralCircular peptidesEnterovirusesEuphorbia hirtaCPE reduction assay |
| spellingShingle | Omonike O. Ogbole Toluwanimi E. Akinleye Abraham O. Nkumah Aminat O. Awogun Alfred F. Attah Moses O. Adewumi Adekunle J. Adeniji In vitro antiviral activity of peptide-rich extracts from seven Nigerian plants against three non-polio enterovirus species C serotypes Virology Journal Antiviral Circular peptides Enteroviruses Euphorbia hirta CPE reduction assay |
| title | In vitro antiviral activity of peptide-rich extracts from seven Nigerian plants against three non-polio enterovirus species C serotypes |
| title_full | In vitro antiviral activity of peptide-rich extracts from seven Nigerian plants against three non-polio enterovirus species C serotypes |
| title_fullStr | In vitro antiviral activity of peptide-rich extracts from seven Nigerian plants against three non-polio enterovirus species C serotypes |
| title_full_unstemmed | In vitro antiviral activity of peptide-rich extracts from seven Nigerian plants against three non-polio enterovirus species C serotypes |
| title_short | In vitro antiviral activity of peptide-rich extracts from seven Nigerian plants against three non-polio enterovirus species C serotypes |
| title_sort | in vitro antiviral activity of peptide rich extracts from seven nigerian plants against three non polio enterovirus species c serotypes |
| topic | Antiviral Circular peptides Enteroviruses Euphorbia hirta CPE reduction assay |
| url | https://doi.org/10.1186/s12985-021-01628-7 |
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