Effects of astaxanthin on microRNA expression in a rat cardiomyocyte anoxia-reoxygenation model
Introduction: The protective effects of astaxanthin against myocardial ischemia-reperfusion injuries are well documented, although the mechanisms are not defined.Methods: The anoxia-reoxygenation injury model was established after astaxanthin treated H9c2 cells for 24 h. Cell viability, lactate dehy...
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1103971/full |
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| author | Xinxin Zhang Xinxin Zhang Xinxin Zhang Min Xu Min Xu Shuilin Cai Bei Chen Hetong Lin Hetong Lin Zhiyu Liu Zhiyu Liu |
| author_facet | Xinxin Zhang Xinxin Zhang Xinxin Zhang Min Xu Min Xu Shuilin Cai Bei Chen Hetong Lin Hetong Lin Zhiyu Liu Zhiyu Liu |
| author_sort | Xinxin Zhang |
| collection | DOAJ |
| description | Introduction: The protective effects of astaxanthin against myocardial ischemia-reperfusion injuries are well documented, although the mechanisms are not defined.Methods: The anoxia-reoxygenation injury model was established after astaxanthin treated H9c2 cells for 24 h. Cell viability, lactate dehydrogenase, oxidative stress level and western blot were tested. Secondly, measured the effects of astaxanthin pretreatment on microRNA expression in a rat myocardial cell anoxia-reoxygenation injury model.Results: After anoxia-reoxygenation injury, in a dose dependent manner, astaxanthin increased cell viability, superoxide dismutase and glutathione peroxidase activity, decreased lactate dehydrogenase and malondialdehyde levels, downregulated protein expression of caspase-3, caspase-8, nuclear factor erythroid-2-related factor 2 and heme oxygenase-1, and upregulated the Bcl-2/Bax ratio. High-throughput sequencing and qPCR showed that microRNAs rno-miR-125b-5p and rno-let-7c-1-3p were differentially expressed (|log2| ≥ 0.585, q < 0.1) between the normal, anoxia-reoxygenation, and astaxanthin (1.25 μM) groups. Kyoto Encyclopedia of Genes and Genomes and GO Gene ontology pathway enrichment analyses showed that TNF signaling, axon guidance, NF-κB signaling pathway, and other pathways displayed differentially expressed microRNA target genes associated with myocardial injuries.Discussion: These results suggested that thetarget genes of rno-miR-125b-5p were enriched in inflammation and apoptosis-related signaling pathways. Also, the results imply that simultaneous targeting of these related signaling pathways could significantly prevent myocardial anoxia-reoxygenation injury in the presence of astaxanthin. |
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| last_indexed | 2024-04-10T17:42:52Z |
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| spelling | doaj.art-1a15c91137114792b412a6788f9f4ba42023-02-03T07:03:28ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-02-011410.3389/fphar.2023.11039711103971Effects of astaxanthin on microRNA expression in a rat cardiomyocyte anoxia-reoxygenation modelXinxin Zhang0Xinxin Zhang1Xinxin Zhang2Min Xu3Min Xu4Shuilin Cai5Bei Chen6Hetong Lin7Hetong Lin8Zhiyu Liu9Zhiyu Liu10College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing, Xiamen, ChinaEngineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing, Xiamen, ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing, Xiamen, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing, Xiamen, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaEngineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing, Xiamen, ChinaIntroduction: The protective effects of astaxanthin against myocardial ischemia-reperfusion injuries are well documented, although the mechanisms are not defined.Methods: The anoxia-reoxygenation injury model was established after astaxanthin treated H9c2 cells for 24 h. Cell viability, lactate dehydrogenase, oxidative stress level and western blot were tested. Secondly, measured the effects of astaxanthin pretreatment on microRNA expression in a rat myocardial cell anoxia-reoxygenation injury model.Results: After anoxia-reoxygenation injury, in a dose dependent manner, astaxanthin increased cell viability, superoxide dismutase and glutathione peroxidase activity, decreased lactate dehydrogenase and malondialdehyde levels, downregulated protein expression of caspase-3, caspase-8, nuclear factor erythroid-2-related factor 2 and heme oxygenase-1, and upregulated the Bcl-2/Bax ratio. High-throughput sequencing and qPCR showed that microRNAs rno-miR-125b-5p and rno-let-7c-1-3p were differentially expressed (|log2| ≥ 0.585, q < 0.1) between the normal, anoxia-reoxygenation, and astaxanthin (1.25 μM) groups. Kyoto Encyclopedia of Genes and Genomes and GO Gene ontology pathway enrichment analyses showed that TNF signaling, axon guidance, NF-κB signaling pathway, and other pathways displayed differentially expressed microRNA target genes associated with myocardial injuries.Discussion: These results suggested that thetarget genes of rno-miR-125b-5p were enriched in inflammation and apoptosis-related signaling pathways. Also, the results imply that simultaneous targeting of these related signaling pathways could significantly prevent myocardial anoxia-reoxygenation injury in the presence of astaxanthin.https://www.frontiersin.org/articles/10.3389/fphar.2023.1103971/fullastaxanthinischemia-reperfusion injurymicroRNAtarget genesantioxidant activity |
| spellingShingle | Xinxin Zhang Xinxin Zhang Xinxin Zhang Min Xu Min Xu Shuilin Cai Bei Chen Hetong Lin Hetong Lin Zhiyu Liu Zhiyu Liu Effects of astaxanthin on microRNA expression in a rat cardiomyocyte anoxia-reoxygenation model Frontiers in Pharmacology astaxanthin ischemia-reperfusion injury microRNA target genes antioxidant activity |
| title | Effects of astaxanthin on microRNA expression in a rat cardiomyocyte anoxia-reoxygenation model |
| title_full | Effects of astaxanthin on microRNA expression in a rat cardiomyocyte anoxia-reoxygenation model |
| title_fullStr | Effects of astaxanthin on microRNA expression in a rat cardiomyocyte anoxia-reoxygenation model |
| title_full_unstemmed | Effects of astaxanthin on microRNA expression in a rat cardiomyocyte anoxia-reoxygenation model |
| title_short | Effects of astaxanthin on microRNA expression in a rat cardiomyocyte anoxia-reoxygenation model |
| title_sort | effects of astaxanthin on microrna expression in a rat cardiomyocyte anoxia reoxygenation model |
| topic | astaxanthin ischemia-reperfusion injury microRNA target genes antioxidant activity |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1103971/full |
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