Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues
Periodontitis is a chronic inflammatory condition that often causes serious damage to tooth-supporting tissues. The limited successful outcomes of clinically available approaches underscore the need for therapeutics that cannot only provide structural guidance to cells but can also modulate the loca...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2023-01-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X22001827 |
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| author | Arwa Daghrery Jessica A. Ferreira Jinping Xu Nasim Golafshan Darnell Kaigler Sarit B. Bhaduri Jos Malda Miguel Castilho Marco C. Bottino |
| author_facet | Arwa Daghrery Jessica A. Ferreira Jinping Xu Nasim Golafshan Darnell Kaigler Sarit B. Bhaduri Jos Malda Miguel Castilho Marco C. Bottino |
| author_sort | Arwa Daghrery |
| collection | DOAJ |
| description | Periodontitis is a chronic inflammatory condition that often causes serious damage to tooth-supporting tissues. The limited successful outcomes of clinically available approaches underscore the need for therapeutics that cannot only provide structural guidance to cells but can also modulate the local immune response. Here, three-dimensional melt electrowritten (i.e., poly(ε-caprolactone)) scaffolds with tissue-specific attributes were engineered to guide differentiation of human-derived periodontal ligament stem cells (hPDLSCs) and mediate macrophage polarization. The investigated tissue-specific scaffold attributes comprised fiber morphology (aligned vs. random) and highly-ordered architectures with distinct strand spacings (small 250 μm and large 500 μm). Macrophages exhibited an elongated morphology in aligned and highly-ordered scaffolds, while maintaining their round-shape on randomly-oriented fibrous scaffolds. Expressions of periostin and IL-10 were more pronounced on the aligned and highly-ordered scaffolds. While hPDLSCs on the scaffolds with 500 μm strand spacing show higher expression of osteogenic marker (Runx2) over 21 days, cells on randomly-oriented fibrous scaffolds showed upregulation of M1 markers. In an orthotopic mandibular fenestration defect model, findings revealed that the tissue-specific scaffolds (i.e., aligned fibers for periodontal ligament and highly-ordered 500 μm strand spacing fluorinated calcium phosphate [F/CaP]-coated fibers for bone) could enhance the mimicking of regeneration of natural periodontal tissues. |
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| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:25:28Z |
| publishDate | 2023-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-1a15eafc6c454208bbfe47c3d4c8c83b2024-04-16T22:17:04ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2023-01-0119268281Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissuesArwa Daghrery0Jessica A. Ferreira1Jinping Xu2Nasim Golafshan3Darnell Kaigler4Sarit B. Bhaduri5Jos Malda6Miguel Castilho7Marco C. Bottino8Department of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, United States; Department of Restorative Dental Sciences, School of Dentistry, Jazan University, Jazan, Kingdom of Saudi ArabiaDepartment of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, United StatesDepartment of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, United StatesDepartment of Orthopedics, University Medical Center Utrecht, Utrecht, the Netherlands; Regenerative Medicine Center, Utrecht, the NetherlandsDepartment of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI, United StatesDepartment of Mechanical, Industrial and Manufacturing Engineering, University of Toledo, Toledo, OH, United States; EEC Division, Directorate of Engineering, The National Science Foundation, Alexandria, VA, United StatesDepartment of Orthopedics, University Medical Center Utrecht, Utrecht, the Netherlands; Regenerative Medicine Center, Utrecht, the Netherlands; Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the NetherlandsDepartment of Orthopedics, University Medical Center Utrecht, Utrecht, the Netherlands; Regenerative Medicine Center, Utrecht, the Netherlands; Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, the Netherlands; Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, the Netherlands; Corresponding author. Biomaterials Design and Processing Eindhoven University of Technology & University Medical Center Utrecht De Rondom 70, 5612, AP Eindhoven, the Netherlands. .Department of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, United States; Department of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, MI, United States; Corresponding author. Regenerative Dentistry University of Michigan School of Dentistry Department of Cariology, Restorative Sciences, and Endodontics 1011 N. University (Room 5223), Ann Arbor, MI, 48109, USA.Periodontitis is a chronic inflammatory condition that often causes serious damage to tooth-supporting tissues. The limited successful outcomes of clinically available approaches underscore the need for therapeutics that cannot only provide structural guidance to cells but can also modulate the local immune response. Here, three-dimensional melt electrowritten (i.e., poly(ε-caprolactone)) scaffolds with tissue-specific attributes were engineered to guide differentiation of human-derived periodontal ligament stem cells (hPDLSCs) and mediate macrophage polarization. The investigated tissue-specific scaffold attributes comprised fiber morphology (aligned vs. random) and highly-ordered architectures with distinct strand spacings (small 250 μm and large 500 μm). Macrophages exhibited an elongated morphology in aligned and highly-ordered scaffolds, while maintaining their round-shape on randomly-oriented fibrous scaffolds. Expressions of periostin and IL-10 were more pronounced on the aligned and highly-ordered scaffolds. While hPDLSCs on the scaffolds with 500 μm strand spacing show higher expression of osteogenic marker (Runx2) over 21 days, cells on randomly-oriented fibrous scaffolds showed upregulation of M1 markers. In an orthotopic mandibular fenestration defect model, findings revealed that the tissue-specific scaffolds (i.e., aligned fibers for periodontal ligament and highly-ordered 500 μm strand spacing fluorinated calcium phosphate [F/CaP]-coated fibers for bone) could enhance the mimicking of regeneration of natural periodontal tissues.http://www.sciencedirect.com/science/article/pii/S2452199X22001827PeriodontitisMelt electrowriting3D printingInflammationPeriodontal regenerationScaffold |
| spellingShingle | Arwa Daghrery Jessica A. Ferreira Jinping Xu Nasim Golafshan Darnell Kaigler Sarit B. Bhaduri Jos Malda Miguel Castilho Marco C. Bottino Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues Bioactive Materials Periodontitis Melt electrowriting 3D printing Inflammation Periodontal regeneration Scaffold |
| title | Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues |
| title_full | Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues |
| title_fullStr | Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues |
| title_full_unstemmed | Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues |
| title_short | Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues |
| title_sort | tissue specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues |
| topic | Periodontitis Melt electrowriting 3D printing Inflammation Periodontal regeneration Scaffold |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X22001827 |
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