Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia
ABSTRACT: Avian coccidiosis causes huge economic losses to the global poultry industry. Vaccine is an important means to prevent and control coccidiosis. In this study, Bacillus subtilis was selected as the expression host strain to express anti Eimeria tenella surface protein SAG22. The synthesized...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123002997 |
| _version_ | 1797770100453933056 |
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| author | Chen Zifan Zheng Chaojun Peng Qiaoli Zhou Qingfeng Du Yunping Zhang Huihua |
| author_facet | Chen Zifan Zheng Chaojun Peng Qiaoli Zhou Qingfeng Du Yunping Zhang Huihua |
| author_sort | Chen Zifan |
| collection | DOAJ |
| description | ABSTRACT: Avian coccidiosis causes huge economic losses to the global poultry industry. Vaccine is an important means to prevent and control coccidiosis. In this study, Bacillus subtilis was selected as the expression host strain to express anti Eimeria tenella surface protein SAG22. The synthesized surface protein SAG22 gene fragment of E. tenella was ligated with Escherichia coli-bacillus shuttle vector GJ148 to construct the recombinant vector SAG22-GJ148. And then the recombinant Bacillus strain SAG22-DH61 was obtained by electrotransfer. The results of SDS-PAGE and Western Blot showed that the recombinant protein SAG22 was successfully expressed intracellularly. The immunoprotective effect of recombinant Bacillus strain SAG22-DH61 on broiler chickens was evaluated in 3 identically designed animal experiments. The birds were infected with E. tenella on d 14, 21, and 28, respectively. Each batch of experiments was divided into 4 groups: blank control group (NC), blank control group + infected E. tenella (CON), addition of recombinant SAG22-DH61 strain + infected with E. tenella (SAG22-DH61), addition of recombinant empty vector GJ148-DH61 strain + infected with E. tenella (GJ148-DH61). The animal experiments results showed that the average weight gain of the SAG22-DH61 group was higher than that of the infected control group, and the difference was significant in the d 14 and 28 attack tests (P < 0.05); the oocyst reduction rate of the SAG22-DH61 group was much higher than that of the GJ148-DH61 group (P < 0.05); the intestinal lesion count score of the SAG22-DH61 group was much lower than that of the GJ148-DH61 group (P < 0.05). In addition, the SAG22-DH61 group achieved highly effective coccidia resistance in the d 14 attack test and moderately effective coccidia resistance in both the d 21 and 28 attack tests. In summary, recombinant SAG22 B. subtilis has the potential to become one of the technological reserves in the prevention and control of coccidiosis in chickens in production. |
| first_indexed | 2024-03-12T21:17:30Z |
| format | Article |
| id | doaj.art-1a16d096a1764f0694eedc2f2621e325 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-12T21:17:30Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj.art-1a16d096a1764f0694eedc2f2621e3252023-07-29T04:34:18ZengElsevierPoultry Science0032-57912023-08-011028102780Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidiaChen Zifan0Zheng Chaojun1Peng Qiaoli2Zhou Qingfeng3Du Yunping4Zhang Huihua5School of Life Science and Engineering, Foshan University, Foshan, 528000, ChinaSchool of Life Science and Engineering, Foshan University, Foshan, 528000, ChinaSchool of Life Science and Engineering, Foshan University, Foshan, 528000, ChinaGuangdong Guangken Institute of Animal Husbandry Engineering Ltd., Wens Food Group Ltd, Yunfu, China, 527300Guangdong Guangken Institute of Animal Husbandry Engineering Ltd., Wens Food Group Ltd, Yunfu, China, 527300Corresponding author:; School of Life Science and Engineering, Foshan University, Foshan, 528000, ChinaABSTRACT: Avian coccidiosis causes huge economic losses to the global poultry industry. Vaccine is an important means to prevent and control coccidiosis. In this study, Bacillus subtilis was selected as the expression host strain to express anti Eimeria tenella surface protein SAG22. The synthesized surface protein SAG22 gene fragment of E. tenella was ligated with Escherichia coli-bacillus shuttle vector GJ148 to construct the recombinant vector SAG22-GJ148. And then the recombinant Bacillus strain SAG22-DH61 was obtained by electrotransfer. The results of SDS-PAGE and Western Blot showed that the recombinant protein SAG22 was successfully expressed intracellularly. The immunoprotective effect of recombinant Bacillus strain SAG22-DH61 on broiler chickens was evaluated in 3 identically designed animal experiments. The birds were infected with E. tenella on d 14, 21, and 28, respectively. Each batch of experiments was divided into 4 groups: blank control group (NC), blank control group + infected E. tenella (CON), addition of recombinant SAG22-DH61 strain + infected with E. tenella (SAG22-DH61), addition of recombinant empty vector GJ148-DH61 strain + infected with E. tenella (GJ148-DH61). The animal experiments results showed that the average weight gain of the SAG22-DH61 group was higher than that of the infected control group, and the difference was significant in the d 14 and 28 attack tests (P < 0.05); the oocyst reduction rate of the SAG22-DH61 group was much higher than that of the GJ148-DH61 group (P < 0.05); the intestinal lesion count score of the SAG22-DH61 group was much lower than that of the GJ148-DH61 group (P < 0.05). In addition, the SAG22-DH61 group achieved highly effective coccidia resistance in the d 14 attack test and moderately effective coccidia resistance in both the d 21 and 28 attack tests. In summary, recombinant SAG22 B. subtilis has the potential to become one of the technological reserves in the prevention and control of coccidiosis in chickens in production.http://www.sciencedirect.com/science/article/pii/S0032579123002997E. tenellaSAG22 proteinimmunological effectBacillus subtilis |
| spellingShingle | Chen Zifan Zheng Chaojun Peng Qiaoli Zhou Qingfeng Du Yunping Zhang Huihua Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia Poultry Science E. tenella SAG22 protein immunological effect Bacillus subtilis |
| title | Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia |
| title_full | Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia |
| title_fullStr | Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia |
| title_full_unstemmed | Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia |
| title_short | Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia |
| title_sort | construction of recombinant sag22 bacillus subtilis and its effect on immune protection of coccidia |
| topic | E. tenella SAG22 protein immunological effect Bacillus subtilis |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123002997 |
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