Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes.
In the present study, we examined the ability of the recombinant spidroin to serve as a substrate for the cardiac tissue engineering. For this purpose, isolated neonatal rat cardiomyocytes were seeded on the electrospun spidroin fiber matrices and cultured to form the confluent cardiac monolayers. B...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4370870?pdf=render |
| _version_ | 1818256950610100224 |
|---|---|
| author | Alexander Teplenin Anna Krasheninnikova Nadezhda Agladze Konstantin Sidoruk Olga Agapova Igor Agapov Vladimir Bogush Konstantin Agladze |
| author_facet | Alexander Teplenin Anna Krasheninnikova Nadezhda Agladze Konstantin Sidoruk Olga Agapova Igor Agapov Vladimir Bogush Konstantin Agladze |
| author_sort | Alexander Teplenin |
| collection | DOAJ |
| description | In the present study, we examined the ability of the recombinant spidroin to serve as a substrate for the cardiac tissue engineering. For this purpose, isolated neonatal rat cardiomyocytes were seeded on the electrospun spidroin fiber matrices and cultured to form the confluent cardiac monolayers. Besides the adhesion assay and immunostaining analysis, we tested the ability of the cultured cardiomyocytes to form a functional cardiac syncytium by studying excitation propagation in the cultured tissue with the aid of optical mapping. It was demonstrated that recombinant spidroin fiber meshes are directly suitable for the adherence and growth of the cardiomyocytes without additional coating with the attachment factors, such as fibronectin. |
| first_indexed | 2024-12-12T17:35:54Z |
| format | Article |
| id | doaj.art-1a18d0ffa0244a85bdbcc9b13f270200 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T17:35:54Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1a18d0ffa0244a85bdbcc9b13f2702002022-12-22T00:17:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e012115510.1371/journal.pone.0121155Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes.Alexander TepleninAnna KrasheninnikovaNadezhda AgladzeKonstantin SidorukOlga AgapovaIgor AgapovVladimir BogushKonstantin AgladzeIn the present study, we examined the ability of the recombinant spidroin to serve as a substrate for the cardiac tissue engineering. For this purpose, isolated neonatal rat cardiomyocytes were seeded on the electrospun spidroin fiber matrices and cultured to form the confluent cardiac monolayers. Besides the adhesion assay and immunostaining analysis, we tested the ability of the cultured cardiomyocytes to form a functional cardiac syncytium by studying excitation propagation in the cultured tissue with the aid of optical mapping. It was demonstrated that recombinant spidroin fiber meshes are directly suitable for the adherence and growth of the cardiomyocytes without additional coating with the attachment factors, such as fibronectin.http://europepmc.org/articles/PMC4370870?pdf=render |
| spellingShingle | Alexander Teplenin Anna Krasheninnikova Nadezhda Agladze Konstantin Sidoruk Olga Agapova Igor Agapov Vladimir Bogush Konstantin Agladze Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes. PLoS ONE |
| title | Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes. |
| title_full | Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes. |
| title_fullStr | Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes. |
| title_full_unstemmed | Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes. |
| title_short | Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes. |
| title_sort | functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes |
| url | http://europepmc.org/articles/PMC4370870?pdf=render |
| work_keys_str_mv | AT alexanderteplenin functionalanalysisoftheengineeredcardiactissuegrownonrecombinantspidroinfibermeshes AT annakrasheninnikova functionalanalysisoftheengineeredcardiactissuegrownonrecombinantspidroinfibermeshes AT nadezhdaagladze functionalanalysisoftheengineeredcardiactissuegrownonrecombinantspidroinfibermeshes AT konstantinsidoruk functionalanalysisoftheengineeredcardiactissuegrownonrecombinantspidroinfibermeshes AT olgaagapova functionalanalysisoftheengineeredcardiactissuegrownonrecombinantspidroinfibermeshes AT igoragapov functionalanalysisoftheengineeredcardiactissuegrownonrecombinantspidroinfibermeshes AT vladimirbogush functionalanalysisoftheengineeredcardiactissuegrownonrecombinantspidroinfibermeshes AT konstantinagladze functionalanalysisoftheengineeredcardiactissuegrownonrecombinantspidroinfibermeshes |