In vitro induction effect of 1,25(OH)2D3 on differentiation of hair follicle stem cell into keratinocyte
Background: Stem cells are characterized by self-renewal and differentiation capabilities. The bulge hair follicle stem cells (HFSCs) are able to convert to epithelial components. The active metabolite of vitamin D, 1,25(OH)2D3, plays important roles in this differentiation process. In the present s...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-02-01
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| Series: | Biomedical Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2319417017300161 |
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| author | Sanaz Joulai Veijouyeh Farhad Mashayekhi Abazar Yari Fatemeh Heidari Nayereh Sajedi Fatemeh Moghani Ghoroghi Maliheh Nobakht |
| author_facet | Sanaz Joulai Veijouyeh Farhad Mashayekhi Abazar Yari Fatemeh Heidari Nayereh Sajedi Fatemeh Moghani Ghoroghi Maliheh Nobakht |
| author_sort | Sanaz Joulai Veijouyeh |
| collection | DOAJ |
| description | Background: Stem cells are characterized by self-renewal and differentiation capabilities. The bulge hair follicle stem cells (HFSCs) are able to convert to epithelial components. The active metabolite of vitamin D, 1,25(OH)2D3, plays important roles in this differentiation process. In the present study has found that 1,25(OH)2D3 induces the HFSCs differentiation into keratinocyte.
Methods: HFSCs are isolated from rat whiskers and cultivated in DMEM medium. To isolate bulge stem cell population, flow cytometry and immunocytochemistry using K15, CD34 and nestin biomarkers were performed. In order to accelerate the HFSCs differentiation into eratinocyte, HFSCs were treated with 10−12 M, 1,25(OH)2D3 every 48 h for a week.
Results: Immunocytochemistry results showed that bulge stem cells are nestin and CD34 positive but K15 negative before differentiation. Subsequently flow cytometry results, showed that the expression of nestin, CD34 and K15 were 70.96%, 93.03% and 6.88% respectively. After differentiation, the immunocytochemical and flow cytometry results indicated that differentiated cells have positive reaction to K15 with 68.94% expression level.
Conclusion: It was concluded that 10−12 M, 1,25(OH)2D3 could induce the HFSCs differentiation into keratinocytes. |
| first_indexed | 2024-12-10T07:32:29Z |
| format | Article |
| id | doaj.art-1a1bf220ed5d4101ac6679c48adf9150 |
| institution | Directory Open Access Journal |
| issn | 2319-4170 |
| language | English |
| last_indexed | 2024-12-10T07:32:29Z |
| publishDate | 2017-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedical Journal |
| spelling | doaj.art-1a1bf220ed5d4101ac6679c48adf91502022-12-22T01:57:31ZengElsevierBiomedical Journal2319-41702017-02-01401313810.1016/j.bj.2016.08.007In vitro induction effect of 1,25(OH)2D3 on differentiation of hair follicle stem cell into keratinocyteSanaz Joulai Veijouyeh0Farhad Mashayekhi1Abazar Yari2Fatemeh Heidari3Nayereh Sajedi4Fatemeh Moghani Ghoroghi5Maliheh Nobakht6Department of Anatomy, School of Medicine, Iran University of Medical Science, Tehran, IranDepartment of Biology, Faculty of Sciences, University of Guilan, Rasht, IranDepartment of Anatomy, School of Medicine, Alborz University of Medical Science, Karaj, IranDepartment of Anatomy, School of Medicine, Qom University of Medical Science, Qom, IranDepartment of Anatomy, School of Medicine, Isfahan University of Medical Science, Isfahan, IranDepartment of Anatomy, School of Medicine, Tehran University of Medical Science, Tehran, IranDepartment of Anatomy, School of Medicine, Iran University of Medical Science, Tehran, IranBackground: Stem cells are characterized by self-renewal and differentiation capabilities. The bulge hair follicle stem cells (HFSCs) are able to convert to epithelial components. The active metabolite of vitamin D, 1,25(OH)2D3, plays important roles in this differentiation process. In the present study has found that 1,25(OH)2D3 induces the HFSCs differentiation into keratinocyte. Methods: HFSCs are isolated from rat whiskers and cultivated in DMEM medium. To isolate bulge stem cell population, flow cytometry and immunocytochemistry using K15, CD34 and nestin biomarkers were performed. In order to accelerate the HFSCs differentiation into eratinocyte, HFSCs were treated with 10−12 M, 1,25(OH)2D3 every 48 h for a week. Results: Immunocytochemistry results showed that bulge stem cells are nestin and CD34 positive but K15 negative before differentiation. Subsequently flow cytometry results, showed that the expression of nestin, CD34 and K15 were 70.96%, 93.03% and 6.88% respectively. After differentiation, the immunocytochemical and flow cytometry results indicated that differentiated cells have positive reaction to K15 with 68.94% expression level. Conclusion: It was concluded that 10−12 M, 1,25(OH)2D3 could induce the HFSCs differentiation into keratinocytes.http://www.sciencedirect.com/science/article/pii/S2319417017300161Hair follicleStem cellBulgeKeratinocyteDifferentiation1,25(OH)2D3 |
| spellingShingle | Sanaz Joulai Veijouyeh Farhad Mashayekhi Abazar Yari Fatemeh Heidari Nayereh Sajedi Fatemeh Moghani Ghoroghi Maliheh Nobakht In vitro induction effect of 1,25(OH)2D3 on differentiation of hair follicle stem cell into keratinocyte Biomedical Journal Hair follicle Stem cell Bulge Keratinocyte Differentiation 1,25(OH)2D3 |
| title | In vitro induction effect of 1,25(OH)2D3 on differentiation of hair follicle stem cell into keratinocyte |
| title_full | In vitro induction effect of 1,25(OH)2D3 on differentiation of hair follicle stem cell into keratinocyte |
| title_fullStr | In vitro induction effect of 1,25(OH)2D3 on differentiation of hair follicle stem cell into keratinocyte |
| title_full_unstemmed | In vitro induction effect of 1,25(OH)2D3 on differentiation of hair follicle stem cell into keratinocyte |
| title_short | In vitro induction effect of 1,25(OH)2D3 on differentiation of hair follicle stem cell into keratinocyte |
| title_sort | in vitro induction effect of 1 25 oh 2d3 on differentiation of hair follicle stem cell into keratinocyte |
| topic | Hair follicle Stem cell Bulge Keratinocyte Differentiation 1,25(OH)2D3 |
| url | http://www.sciencedirect.com/science/article/pii/S2319417017300161 |
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