Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with <i>Neochloris oleoabundans</i>
Extractive fermentation is an in situ method for the production and recovery of biomolecules of interest. Aqueous two-phase systems (ATPS) allow the product to be recovered in one phase of the system, reducing unit operations in the bioprocess. Thermosensitive polymers such as EOPOs are an interesti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/3/130 |
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| author | Ana Patricia Garza-Chapa Carlos Iván Ávila-Velasco José González-Valdez Alma Gómez-Loredo |
| author_facet | Ana Patricia Garza-Chapa Carlos Iván Ávila-Velasco José González-Valdez Alma Gómez-Loredo |
| author_sort | Ana Patricia Garza-Chapa |
| collection | DOAJ |
| description | Extractive fermentation is an in situ method for the production and recovery of biomolecules of interest. Aqueous two-phase systems (ATPS) allow the product to be recovered in one phase of the system, reducing unit operations in the bioprocess. Thermosensitive polymers such as EOPOs are an interesting alternative to be applied in ATPS. In this work, different EOPOs were tested in an extractive fermentation strategy with the green microalgae <i>Neochloris oleoabundans</i> to provide a basis for future implementations of these systems in microalgae bioprocesses. Extractive fermentations were carried out with two EOPOs of different molecular weights (3900 and 12,000 g/mol) at concentrations of 10% and 15% (<i>w</i>/<i>v</i>). The microalga was incubated axenically under two different sets of conditions for 21 and 45 days, respectively. Cell counts were performed, and cell growth curves were obtained. Additionally, a semi-continuous and batch extractive fermentation assay was performed. The extractive fermentation with EOPO showed lower cell growth and a longer adaptation time of the microalgae in the fermentation, and EPS production yields of up to 8–23 g/L were obtained. Extractive fermentation is an interesting method to be implemented in microalgae cultures; however, further conditions need to be explored to achieve an appropriate bioprocess. |
| first_indexed | 2024-04-24T18:17:41Z |
| format | Article |
| id | doaj.art-1a1c651ecf0d42139d1f3494a7c3178a |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-04-24T18:17:41Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-1a1c651ecf0d42139d1f3494a7c3178a2024-03-27T13:37:55ZengMDPI AGFermentation2311-56372024-02-0110313010.3390/fermentation10030130Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with <i>Neochloris oleoabundans</i>Ana Patricia Garza-Chapa0Carlos Iván Ávila-Velasco1José González-Valdez2Alma Gómez-Loredo3Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Pedro de Alba, S/N, San Nicolas de los Garza 66455, NL, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Pedro de Alba, S/N, San Nicolas de los Garza 66455, NL, MexicoTecnologico de Monterrey, School of Engineering and Science, Ave. Eugenio Garza Sada 2501 Sur, Monterrey 64849, NL, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Pedro de Alba, S/N, San Nicolas de los Garza 66455, NL, MexicoExtractive fermentation is an in situ method for the production and recovery of biomolecules of interest. Aqueous two-phase systems (ATPS) allow the product to be recovered in one phase of the system, reducing unit operations in the bioprocess. Thermosensitive polymers such as EOPOs are an interesting alternative to be applied in ATPS. In this work, different EOPOs were tested in an extractive fermentation strategy with the green microalgae <i>Neochloris oleoabundans</i> to provide a basis for future implementations of these systems in microalgae bioprocesses. Extractive fermentations were carried out with two EOPOs of different molecular weights (3900 and 12,000 g/mol) at concentrations of 10% and 15% (<i>w</i>/<i>v</i>). The microalga was incubated axenically under two different sets of conditions for 21 and 45 days, respectively. Cell counts were performed, and cell growth curves were obtained. Additionally, a semi-continuous and batch extractive fermentation assay was performed. The extractive fermentation with EOPO showed lower cell growth and a longer adaptation time of the microalgae in the fermentation, and EPS production yields of up to 8–23 g/L were obtained. Extractive fermentation is an interesting method to be implemented in microalgae cultures; however, further conditions need to be explored to achieve an appropriate bioprocess.https://www.mdpi.com/2311-5637/10/3/130extractive fermentationaqueous two-phase systemthermoseparating polymers<i>Neochloris oleoabundans</i> |
| spellingShingle | Ana Patricia Garza-Chapa Carlos Iván Ávila-Velasco José González-Valdez Alma Gómez-Loredo Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with <i>Neochloris oleoabundans</i> Fermentation extractive fermentation aqueous two-phase system thermoseparating polymers <i>Neochloris oleoabundans</i> |
| title | Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with <i>Neochloris oleoabundans</i> |
| title_full | Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with <i>Neochloris oleoabundans</i> |
| title_fullStr | Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with <i>Neochloris oleoabundans</i> |
| title_full_unstemmed | Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with <i>Neochloris oleoabundans</i> |
| title_short | Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with <i>Neochloris oleoabundans</i> |
| title_sort | application of aqueous two phase systems with thermoseparating polymers eopo as a method for extractive fermentation with i neochloris oleoabundans i |
| topic | extractive fermentation aqueous two-phase system thermoseparating polymers <i>Neochloris oleoabundans</i> |
| url | https://www.mdpi.com/2311-5637/10/3/130 |
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