<i>Klebsiella aerogenes</i> Adhesion Behaviour during Biofilm Formation on Monazite
The adsorption behaviour of micro-organisms during the initial attachment stage of biofilm formation affects subsequent stages. The available area for attachment and the chemophysical properties of a surface affect microbial attachment performance. This study focused on the initial attachment behavi...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/5/1331 |
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| author | Arya Van Alin Melissa K. Corbett Homayoun Fathollahzadeh M. Christian Tjiam Andrew Putnis Jacques Eksteen Anna H. Kaksonen Elizabeth Watkin |
| author_facet | Arya Van Alin Melissa K. Corbett Homayoun Fathollahzadeh M. Christian Tjiam Andrew Putnis Jacques Eksteen Anna H. Kaksonen Elizabeth Watkin |
| author_sort | Arya Van Alin |
| collection | DOAJ |
| description | The adsorption behaviour of micro-organisms during the initial attachment stage of biofilm formation affects subsequent stages. The available area for attachment and the chemophysical properties of a surface affect microbial attachment performance. This study focused on the initial attachment behaviour of <i>Klebsiella aerogenes</i> on monazite by measuring the ratio of planktonic against sessile subpopulations (P:S ratio), and the potential role of extracellular DNA (eDNA). eDNA production, effects of physicochemical properties of the surface, particle size, total available area for attachment, and the initial inoculation size on the attachment behaviour were tested. <i>K. aerogenes</i> attached to monazite immediately after exposure to the ore; however, the P:S ratio significantly (<i>p</i> = 0.05) changed in response to the particle size, available area, and inoculation size. Attachment occurred preferentially on larger-sized (~50 µm) particles, and either decreasing the inoculation size or increasing the available area further promoted attachment. Nevertheless, a portion of the inoculated cells always remained in a planktonic state. <i>K. aerogenes</i> produced lower eDNA in response to the changed surface chemical properties when monazite was replaced by xenotime. Using pure eDNA to cover the monazite surface significantly (<i>p</i> ≤ 0.05) hindered bacterial attachment due to the repulsive interaction between the eDNA layer and bacteria. |
| first_indexed | 2024-03-11T03:28:04Z |
| format | Article |
| id | doaj.art-1a1cb94c6fb14f14b070605eea648a43 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T03:28:04Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-1a1cb94c6fb14f14b070605eea648a432023-11-18T02:35:14ZengMDPI AGMicroorganisms2076-26072023-05-01115133110.3390/microorganisms11051331<i>Klebsiella aerogenes</i> Adhesion Behaviour during Biofilm Formation on MonaziteArya Van Alin0Melissa K. Corbett1Homayoun Fathollahzadeh2M. Christian Tjiam3Andrew Putnis4Jacques Eksteen5Anna H. Kaksonen6Elizabeth Watkin7Curtin Medical School, Curtin University, Bentley, WA 6102, AustraliaCurtin Medical School, Curtin University, Bentley, WA 6102, AustraliaCurtin Medical School, Curtin University, Bentley, WA 6102, AustraliaCurtin Medical School, Curtin University, Bentley, WA 6102, AustraliaThe Institute for Geoscience Research, School of Earth and Planetary Sciences, Curtin University, Bentley, WA 6102, AustraliaWA School of Mines, Minerals, Energy and Chemical Engineering, Curtin University, Waterford, WA 6152, AustraliaWA School of Mines, Minerals, Energy and Chemical Engineering, Curtin University, Waterford, WA 6152, AustraliaCurtin Medical School, Curtin University, Bentley, WA 6102, AustraliaThe adsorption behaviour of micro-organisms during the initial attachment stage of biofilm formation affects subsequent stages. The available area for attachment and the chemophysical properties of a surface affect microbial attachment performance. This study focused on the initial attachment behaviour of <i>Klebsiella aerogenes</i> on monazite by measuring the ratio of planktonic against sessile subpopulations (P:S ratio), and the potential role of extracellular DNA (eDNA). eDNA production, effects of physicochemical properties of the surface, particle size, total available area for attachment, and the initial inoculation size on the attachment behaviour were tested. <i>K. aerogenes</i> attached to monazite immediately after exposure to the ore; however, the P:S ratio significantly (<i>p</i> = 0.05) changed in response to the particle size, available area, and inoculation size. Attachment occurred preferentially on larger-sized (~50 µm) particles, and either decreasing the inoculation size or increasing the available area further promoted attachment. Nevertheless, a portion of the inoculated cells always remained in a planktonic state. <i>K. aerogenes</i> produced lower eDNA in response to the changed surface chemical properties when monazite was replaced by xenotime. Using pure eDNA to cover the monazite surface significantly (<i>p</i> ≤ 0.05) hindered bacterial attachment due to the repulsive interaction between the eDNA layer and bacteria.https://www.mdpi.com/2076-2607/11/5/1331biofilm<i>Klebsiella aerogenes</i>monaziteextracellular DNA (eDNA)surface attachment |
| spellingShingle | Arya Van Alin Melissa K. Corbett Homayoun Fathollahzadeh M. Christian Tjiam Andrew Putnis Jacques Eksteen Anna H. Kaksonen Elizabeth Watkin <i>Klebsiella aerogenes</i> Adhesion Behaviour during Biofilm Formation on Monazite Microorganisms biofilm <i>Klebsiella aerogenes</i> monazite extracellular DNA (eDNA) surface attachment |
| title | <i>Klebsiella aerogenes</i> Adhesion Behaviour during Biofilm Formation on Monazite |
| title_full | <i>Klebsiella aerogenes</i> Adhesion Behaviour during Biofilm Formation on Monazite |
| title_fullStr | <i>Klebsiella aerogenes</i> Adhesion Behaviour during Biofilm Formation on Monazite |
| title_full_unstemmed | <i>Klebsiella aerogenes</i> Adhesion Behaviour during Biofilm Formation on Monazite |
| title_short | <i>Klebsiella aerogenes</i> Adhesion Behaviour during Biofilm Formation on Monazite |
| title_sort | i klebsiella aerogenes i adhesion behaviour during biofilm formation on monazite |
| topic | biofilm <i>Klebsiella aerogenes</i> monazite extracellular DNA (eDNA) surface attachment |
| url | https://www.mdpi.com/2076-2607/11/5/1331 |
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