Optimization of β-galactosidase production from lactic acid bacteria
β-galactosidase, commonly known as lactase, represents commercially important enzyme that is prevalently used for lactose hydrolysis in milk and whey. To the date, it has been isolated from various sources. In this study different strains of lactic acid bacteria were assessed for their β-ga...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Association of Chemical Engineers of Serbia
2015-01-01
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| Series: | Hemijska Industrija |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0367-598X/2015/0367-598X1400044C.pdf |
| Summary: | β-galactosidase, commonly known as lactase, represents commercially important
enzyme that is prevalently used for lactose hydrolysis in milk and whey. To
the date, it has been isolated from various sources. In this study different
strains of lactic acid bacteria were assessed for their β-galactosidase
productivity, and Lactobacillus acidophilus ATCC 4356 resulted with the
highest production potential. Thereafter, optimal conditions for
accomplishing high yields of β-galactosidase activity were determined.
Maximal specific activity (1.01 IU mL-1) was accomplished after 2 days shake
flask culture fermentation (150 rpm) at 37ºC, with modified Man Rogosa Sharpe
culture broth using lactose (2.5%) as sole carbon source. Finally, in order
to intensify release of intracellular β-galactosidase different mechanical
and chemical methods were conducted. Nevertheless, vortexing with quartz sand
(150 μm) as abrasive was proven to be the most efficient method of cell
disruption. The optimum temperature of obtained β-galactosidase was 45°C and
the optimum range pH 6.5-7.5. |
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| ISSN: | 0367-598X 2217-7426 |