Transcriptome analysis reveals anthocyanin regulation in Chinese cabbage (Brassica rapa L.) at low temperatures
Abstract Chinese cabbage that prefers cold conditions is also affected by low-temperature stress, such as the accumulation of leaf anthocyanins. Research on anthocyanin biosynthesis and regulation mechanisms has made great progress. However, research on anthocyanin accumulation for resistance to bio...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-10106-1 |
| _version_ | 1817990732810551296 |
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| author | Yun Dai Lei Zhang Xiao Sun Fei Li Shifan Zhang Hui Zhang Guoliang Li Zhiyuan Fang Rifei Sun Xilin Hou Shujiang Zhang |
| author_facet | Yun Dai Lei Zhang Xiao Sun Fei Li Shifan Zhang Hui Zhang Guoliang Li Zhiyuan Fang Rifei Sun Xilin Hou Shujiang Zhang |
| author_sort | Yun Dai |
| collection | DOAJ |
| description | Abstract Chinese cabbage that prefers cold conditions is also affected by low-temperature stress, such as the accumulation of leaf anthocyanins. Research on anthocyanin biosynthesis and regulation mechanisms has made great progress. However, research on anthocyanin accumulation for resistance to biological and non-biological stress is still lacking. To study the relationship between anthocyanin accumulation of Chinese cabbage and resistance under low-temperature conditions, RNA sequencing (RNA-seq) was performed on Chinese cabbage ‘Xiao Baojian’ grown at a low temperature for four time periods and at a control temperature for five time periods. In Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, 7954 differentially expressed genes (DEGs) were enriched, of which 587 DEGs belonged to "biosynthesis of other secondary metabolites." Gene temporal expression patterns were used to discover enriched genes related to phenylpropanoid biosynthesis; flavonoid biosynthesis and anthocyanin biosynthesis pathways were found in cluster 1. The interaction networks were constructed, and hub genes were selected, showing that flavonoid biosynthesis pathway genes (DFR, ANS, F3H, FLS1, CHS1, CHS3, and TT8) and defense mechanisms-related genes (DFR, SNL6, and TKPR1) interact with each other. Anthocyanin biosynthesis DEGs in Chinese cabbage were evaluated under low-temperature conditions to map the relevant pathways, and expression maps of transcription factors in the flavonoid pathway were created at various periods. Low temperature upregulated the expression of genes related to anthocyanin biosynthesis. Taken together, our results provide further analysis of the relationship between plant anthocyanin synthesis and stress resistance and may also provide further insights for the future development of high-quality color and cold-tolerant Chinese cabbage germplasm resources. |
| first_indexed | 2024-04-14T01:03:05Z |
| format | Article |
| id | doaj.art-1a203054780741e885a7cf457227b138 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-14T01:03:05Z |
| publishDate | 2022-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1a203054780741e885a7cf457227b1382022-12-22T02:21:20ZengNature PortfolioScientific Reports2045-23222022-04-0112111510.1038/s41598-022-10106-1Transcriptome analysis reveals anthocyanin regulation in Chinese cabbage (Brassica rapa L.) at low temperaturesYun Dai0Lei Zhang1Xiao Sun2Fei Li3Shifan Zhang4Hui Zhang5Guoliang Li6Zhiyuan Fang7Rifei Sun8Xilin Hou9Shujiang Zhang10Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesAbstract Chinese cabbage that prefers cold conditions is also affected by low-temperature stress, such as the accumulation of leaf anthocyanins. Research on anthocyanin biosynthesis and regulation mechanisms has made great progress. However, research on anthocyanin accumulation for resistance to biological and non-biological stress is still lacking. To study the relationship between anthocyanin accumulation of Chinese cabbage and resistance under low-temperature conditions, RNA sequencing (RNA-seq) was performed on Chinese cabbage ‘Xiao Baojian’ grown at a low temperature for four time periods and at a control temperature for five time periods. In Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, 7954 differentially expressed genes (DEGs) were enriched, of which 587 DEGs belonged to "biosynthesis of other secondary metabolites." Gene temporal expression patterns were used to discover enriched genes related to phenylpropanoid biosynthesis; flavonoid biosynthesis and anthocyanin biosynthesis pathways were found in cluster 1. The interaction networks were constructed, and hub genes were selected, showing that flavonoid biosynthesis pathway genes (DFR, ANS, F3H, FLS1, CHS1, CHS3, and TT8) and defense mechanisms-related genes (DFR, SNL6, and TKPR1) interact with each other. Anthocyanin biosynthesis DEGs in Chinese cabbage were evaluated under low-temperature conditions to map the relevant pathways, and expression maps of transcription factors in the flavonoid pathway were created at various periods. Low temperature upregulated the expression of genes related to anthocyanin biosynthesis. Taken together, our results provide further analysis of the relationship between plant anthocyanin synthesis and stress resistance and may also provide further insights for the future development of high-quality color and cold-tolerant Chinese cabbage germplasm resources.https://doi.org/10.1038/s41598-022-10106-1 |
| spellingShingle | Yun Dai Lei Zhang Xiao Sun Fei Li Shifan Zhang Hui Zhang Guoliang Li Zhiyuan Fang Rifei Sun Xilin Hou Shujiang Zhang Transcriptome analysis reveals anthocyanin regulation in Chinese cabbage (Brassica rapa L.) at low temperatures Scientific Reports |
| title | Transcriptome analysis reveals anthocyanin regulation in Chinese cabbage (Brassica rapa L.) at low temperatures |
| title_full | Transcriptome analysis reveals anthocyanin regulation in Chinese cabbage (Brassica rapa L.) at low temperatures |
| title_fullStr | Transcriptome analysis reveals anthocyanin regulation in Chinese cabbage (Brassica rapa L.) at low temperatures |
| title_full_unstemmed | Transcriptome analysis reveals anthocyanin regulation in Chinese cabbage (Brassica rapa L.) at low temperatures |
| title_short | Transcriptome analysis reveals anthocyanin regulation in Chinese cabbage (Brassica rapa L.) at low temperatures |
| title_sort | transcriptome analysis reveals anthocyanin regulation in chinese cabbage brassica rapa l at low temperatures |
| url | https://doi.org/10.1038/s41598-022-10106-1 |
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