Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro
Japanese encephalitis virus (JEV) is a zoonotic mosquito-borne flavivirus which is the leading causative agent of viral encephalitis in endemic regions. JEV NS3 is a component of the viral replicase complex and is a multifunctional protein. In this study, interleukin enhancer-binding factor 2 (ILF2)...
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| Format: | Article |
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MDPI AG
2019-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/6/559 |
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| author | Xiaofang Cui Ping Qian Tingting Rao Yanming Wei Fang Zhao Huawei Zhang Huanchun Chen Xiangmin Li |
| author_facet | Xiaofang Cui Ping Qian Tingting Rao Yanming Wei Fang Zhao Huawei Zhang Huanchun Chen Xiangmin Li |
| author_sort | Xiaofang Cui |
| collection | DOAJ |
| description | Japanese encephalitis virus (JEV) is a zoonotic mosquito-borne flavivirus which is the leading causative agent of viral encephalitis in endemic regions. JEV NS3 is a component of the viral replicase complex and is a multifunctional protein. In this study, interleukin enhancer-binding factor 2 (ILF2) is identified as a novel cellular protein interacting with NS3 through co-immunoprecipitation assay and LC-MS/MS. The expression of ILF2 is decreased in JEV-infected human embryonic kidney (293T) cells. The knockdown of endogenous ILF2 by special short hairpin RNA (shRNA) positively regulates JEV propagation, whereas the overexpression of ILF2 results in a significantly reduced JEV genome synthesis. Further analysis revealed that the knockdown of ILF2 positively regulates viral replication by JEV replicon system studies. These results suggest that ILF2 may act as a potential antiviral agent against JEV infection. |
| first_indexed | 2024-12-20T11:40:41Z |
| format | Article |
| id | doaj.art-1a217c68d2344a26a8bca08e02019b12 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-20T11:40:41Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1a217c68d2344a26a8bca08e02019b122022-12-21T19:42:00ZengMDPI AGViruses1999-49152019-06-0111655910.3390/v11060559v11060559Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In VitroXiaofang Cui0Ping Qian1Tingting Rao2Yanming Wei3Fang Zhao4Huawei Zhang5Huanchun Chen6Xiangmin Li7State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaJapanese encephalitis virus (JEV) is a zoonotic mosquito-borne flavivirus which is the leading causative agent of viral encephalitis in endemic regions. JEV NS3 is a component of the viral replicase complex and is a multifunctional protein. In this study, interleukin enhancer-binding factor 2 (ILF2) is identified as a novel cellular protein interacting with NS3 through co-immunoprecipitation assay and LC-MS/MS. The expression of ILF2 is decreased in JEV-infected human embryonic kidney (293T) cells. The knockdown of endogenous ILF2 by special short hairpin RNA (shRNA) positively regulates JEV propagation, whereas the overexpression of ILF2 results in a significantly reduced JEV genome synthesis. Further analysis revealed that the knockdown of ILF2 positively regulates viral replication by JEV replicon system studies. These results suggest that ILF2 may act as a potential antiviral agent against JEV infection.https://www.mdpi.com/1999-4915/11/6/559Japanese encephalitis viruscellular interleukin enhancer-binding factor 2 (ILF2)antiviral activityviral RNA translation |
| spellingShingle | Xiaofang Cui Ping Qian Tingting Rao Yanming Wei Fang Zhao Huawei Zhang Huanchun Chen Xiangmin Li Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro Viruses Japanese encephalitis virus cellular interleukin enhancer-binding factor 2 (ILF2) antiviral activity viral RNA translation |
| title | Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro |
| title_full | Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro |
| title_fullStr | Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro |
| title_full_unstemmed | Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro |
| title_short | Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro |
| title_sort | cellular interleukin enhancer binding factor 2 ilf2 inhibits japanese encephalitis virus replication in vitro |
| topic | Japanese encephalitis virus cellular interleukin enhancer-binding factor 2 (ILF2) antiviral activity viral RNA translation |
| url | https://www.mdpi.com/1999-4915/11/6/559 |
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