A comparative study of in vitro antimicrobial, antioxidant and cytotoxic activity of Albizia lebbeck and Acacia nilotica stem bark
Petroleum ether, ethyl acetate and methanol extracts of the stem bark of Albizia lebbeck and Acacia nilotica was investigated for antimicrobial, antioxidant and cytotoxicity assay. The powdered stem bark was extracted successively with petroleum ether, ethyl acetate and methanol solution. The crude...
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Faculty of Pharmacy, Cairo University
2018-06-01
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| Series: | Bulletin of Faculty of Pharmacy Cairo University |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110093117300479 |
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| author | Mohammad Tuhin Ali Sheikh Tanzina Haque Md. Lutful Kabir Sohel Rana Md. Enamul Haque |
| author_facet | Mohammad Tuhin Ali Sheikh Tanzina Haque Md. Lutful Kabir Sohel Rana Md. Enamul Haque |
| author_sort | Mohammad Tuhin Ali |
| collection | DOAJ |
| description | Petroleum ether, ethyl acetate and methanol extracts of the stem bark of Albizia lebbeck and Acacia nilotica was investigated for antimicrobial, antioxidant and cytotoxicity assay. The powdered stem bark was extracted successively with petroleum ether, ethyl acetate and methanol solution. The crude extracts were subjected to antimicrobial, antioxidant and cytotoxicity assay by using disc diffusion method, DPPH and hydroxyl free radical scavenging assay, and brine shrimp lethality test, respectively. The petroleum ether and ethyl acetate extracts of both plants showed the most prominent activity in antimicrobial susceptibility test as compared to methanol extracts. These extracts showed moderate activity against the Bacillus subtilis, Staphylococcus aureus, Vibrio mimicus, Salmonella typhi, Shigella dysenteriae, Candida arrizae and Aspergillus niger. The zone of inhibition against the tested bacteria and fungi were 11–14 mm and 8–10 mm, respectively. The ethyl acetate extract of both plants showed the highest antioxidant and cytotoxic activity when compared with that of petroleum ether and methanol extracts. All the tested extracts showed higher free radical scavenging activity than that of the standard, ascorbic acid. In compared with A. lebbeck, A. nilotica was found to have the lower IC50 value which was 74.29 µg/ml and 68.03 µg/ml in DPPH and hydroxyl free radical scavenging assay, respectively. The LC50 value was 42.36 and 37.32 µg/ml for the ethyl acetate extract of A. lebbeck and A. nilotica, respectively. We suggest further study for the identification of active compounds from the bark extracts of A. lebbeck and A. nilotica. |
| first_indexed | 2024-04-11T02:53:45Z |
| format | Article |
| id | doaj.art-1a22f4da488642aa9a7a8542f4be0e3e |
| institution | Directory Open Access Journal |
| issn | 1110-0931 |
| language | English |
| last_indexed | 2024-04-11T02:53:45Z |
| publishDate | 2018-06-01 |
| publisher | Faculty of Pharmacy, Cairo University |
| record_format | Article |
| series | Bulletin of Faculty of Pharmacy Cairo University |
| spelling | doaj.art-1a22f4da488642aa9a7a8542f4be0e3e2023-01-02T15:34:24ZengFaculty of Pharmacy, Cairo UniversityBulletin of Faculty of Pharmacy Cairo University1110-09312018-06-01561343810.1016/j.bfopcu.2017.10.002A comparative study of in vitro antimicrobial, antioxidant and cytotoxic activity of Albizia lebbeck and Acacia nilotica stem barkMohammad Tuhin Ali0Sheikh Tanzina Haque1Md. Lutful Kabir2Sohel Rana3Md. Enamul Haque4Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, BangladeshDepartment of Applied Chemistry and Chemical Engineering, University of Dhaka, Dhaka 1000, BangladeshDept. of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Science & Technology University, Gopalganj 8100, BangladeshDepartment of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, BangladeshDepartment of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, BangladeshPetroleum ether, ethyl acetate and methanol extracts of the stem bark of Albizia lebbeck and Acacia nilotica was investigated for antimicrobial, antioxidant and cytotoxicity assay. The powdered stem bark was extracted successively with petroleum ether, ethyl acetate and methanol solution. The crude extracts were subjected to antimicrobial, antioxidant and cytotoxicity assay by using disc diffusion method, DPPH and hydroxyl free radical scavenging assay, and brine shrimp lethality test, respectively. The petroleum ether and ethyl acetate extracts of both plants showed the most prominent activity in antimicrobial susceptibility test as compared to methanol extracts. These extracts showed moderate activity against the Bacillus subtilis, Staphylococcus aureus, Vibrio mimicus, Salmonella typhi, Shigella dysenteriae, Candida arrizae and Aspergillus niger. The zone of inhibition against the tested bacteria and fungi were 11–14 mm and 8–10 mm, respectively. The ethyl acetate extract of both plants showed the highest antioxidant and cytotoxic activity when compared with that of petroleum ether and methanol extracts. All the tested extracts showed higher free radical scavenging activity than that of the standard, ascorbic acid. In compared with A. lebbeck, A. nilotica was found to have the lower IC50 value which was 74.29 µg/ml and 68.03 µg/ml in DPPH and hydroxyl free radical scavenging assay, respectively. The LC50 value was 42.36 and 37.32 µg/ml for the ethyl acetate extract of A. lebbeck and A. nilotica, respectively. We suggest further study for the identification of active compounds from the bark extracts of A. lebbeck and A. nilotica.http://www.sciencedirect.com/science/article/pii/S1110093117300479Albizia lebbeckAcacia niloticaAntimicrobial assayAntioxidant assayCytotoxicity test |
| spellingShingle | Mohammad Tuhin Ali Sheikh Tanzina Haque Md. Lutful Kabir Sohel Rana Md. Enamul Haque A comparative study of in vitro antimicrobial, antioxidant and cytotoxic activity of Albizia lebbeck and Acacia nilotica stem bark Bulletin of Faculty of Pharmacy Cairo University Albizia lebbeck Acacia nilotica Antimicrobial assay Antioxidant assay Cytotoxicity test |
| title | A comparative study of in vitro antimicrobial, antioxidant and cytotoxic activity of Albizia lebbeck and Acacia nilotica stem bark |
| title_full | A comparative study of in vitro antimicrobial, antioxidant and cytotoxic activity of Albizia lebbeck and Acacia nilotica stem bark |
| title_fullStr | A comparative study of in vitro antimicrobial, antioxidant and cytotoxic activity of Albizia lebbeck and Acacia nilotica stem bark |
| title_full_unstemmed | A comparative study of in vitro antimicrobial, antioxidant and cytotoxic activity of Albizia lebbeck and Acacia nilotica stem bark |
| title_short | A comparative study of in vitro antimicrobial, antioxidant and cytotoxic activity of Albizia lebbeck and Acacia nilotica stem bark |
| title_sort | comparative study of in vitro antimicrobial antioxidant and cytotoxic activity of albizia lebbeck and acacia nilotica stem bark |
| topic | Albizia lebbeck Acacia nilotica Antimicrobial assay Antioxidant assay Cytotoxicity test |
| url | http://www.sciencedirect.com/science/article/pii/S1110093117300479 |
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