Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei
Solanum betaceum is a tree from the Andean region bearing edible fruits, considered an exotic export. Although there has been renewed interest in its commercialization, sustainability, and disease management have been limiting factors. Phytophthora betacei is a recently described species that causes...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.730251/full |
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| author | Daniel Bautista Natalia Guayazan-Palacios Natalia Guayazan-Palacios Maria Camila Buitrago Martha Cardenas David Botero Jorge Duitama Adriana J. Bernal Silvia Restrepo |
| author_facet | Daniel Bautista Natalia Guayazan-Palacios Natalia Guayazan-Palacios Maria Camila Buitrago Martha Cardenas David Botero Jorge Duitama Adriana J. Bernal Silvia Restrepo |
| author_sort | Daniel Bautista |
| collection | DOAJ |
| description | Solanum betaceum is a tree from the Andean region bearing edible fruits, considered an exotic export. Although there has been renewed interest in its commercialization, sustainability, and disease management have been limiting factors. Phytophthora betacei is a recently described species that causes late blight in S. betaceum. There is no general study of the response of S. betaceum, particularly, in the changes in expression of pathogenesis-related genes. In this manuscript we present a comprehensive RNA-seq time-series study of the plant response to the infection of P. betacei. Following six time points of infection, the differentially expressed genes (DEGs) involved in the defense by the plant were contextualized in a sequential manner. We documented 5,628 DEGs across all time-points. From 6 to 24 h post-inoculation, we highlighted DEGs involved in the recognition of the pathogen by the likely activation of pattern-triggered immunity (PTI) genes. We also describe the possible effect of the pathogen effectors in the host during the effector-triggered response. Finally, we reveal genes related to the susceptible outcome of the interaction caused by the onset of necrotrophy and the sharp transcriptional changes as a response to the pathogen. This is the first report of the transcriptome of the tree tomato in response to the newly described pathogen P. betacei. |
| first_indexed | 2024-12-21T09:10:56Z |
| format | Article |
| id | doaj.art-1a23e69238544a23a30cef8c53e6249a |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T09:10:56Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1a23e69238544a23a30cef8c53e6249a2022-12-21T19:09:14ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-10-011210.3389/fpls.2021.730251730251Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betaceiDaniel Bautista0Natalia Guayazan-Palacios1Natalia Guayazan-Palacios2Maria Camila Buitrago3Martha Cardenas4David Botero5Jorge Duitama6Adriana J. Bernal7Silvia Restrepo8Department of Biological Sciences, Universidad de los Andes, Bogotá, ColombiaDepartment of Biological Sciences, Universidad de los Andes, Bogotá, ColombiaDepartment of Biology, University of Washington, Seattle, WA, United StatesDepartment of Biological Sciences, Universidad de los Andes, Bogotá, ColombiaDepartment of Biological Sciences, Universidad de los Andes, Bogotá, ColombiaDepartment of Biological Sciences, Universidad de los Andes, Bogotá, ColombiaDepartment of Systems and Computing Engineering, Universidad de los Andes, Bogotá, ColombiaDepartment of Biological Sciences, Universidad de los Andes, Bogotá, ColombiaDepartment of Chemical and Food Engineering, Universidad de los Andes, Bogotá, ColombiaSolanum betaceum is a tree from the Andean region bearing edible fruits, considered an exotic export. Although there has been renewed interest in its commercialization, sustainability, and disease management have been limiting factors. Phytophthora betacei is a recently described species that causes late blight in S. betaceum. There is no general study of the response of S. betaceum, particularly, in the changes in expression of pathogenesis-related genes. In this manuscript we present a comprehensive RNA-seq time-series study of the plant response to the infection of P. betacei. Following six time points of infection, the differentially expressed genes (DEGs) involved in the defense by the plant were contextualized in a sequential manner. We documented 5,628 DEGs across all time-points. From 6 to 24 h post-inoculation, we highlighted DEGs involved in the recognition of the pathogen by the likely activation of pattern-triggered immunity (PTI) genes. We also describe the possible effect of the pathogen effectors in the host during the effector-triggered response. Finally, we reveal genes related to the susceptible outcome of the interaction caused by the onset of necrotrophy and the sharp transcriptional changes as a response to the pathogen. This is the first report of the transcriptome of the tree tomato in response to the newly described pathogen P. betacei.https://www.frontiersin.org/articles/10.3389/fpls.2021.730251/fullPhytophthora betaceiplant–pathogen interactionplant responseSolanum betaceumtime series analysis |
| spellingShingle | Daniel Bautista Natalia Guayazan-Palacios Natalia Guayazan-Palacios Maria Camila Buitrago Martha Cardenas David Botero Jorge Duitama Adriana J. Bernal Silvia Restrepo Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei Frontiers in Plant Science Phytophthora betacei plant–pathogen interaction plant response Solanum betaceum time series analysis |
| title | Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei |
| title_full | Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei |
| title_fullStr | Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei |
| title_full_unstemmed | Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei |
| title_short | Comprehensive Time-Series Analysis of the Gene Expression Profile in a Susceptible Cultivar of Tree Tomato (Solanum betaceum) During the Infection of Phytophthora betacei |
| title_sort | comprehensive time series analysis of the gene expression profile in a susceptible cultivar of tree tomato solanum betaceum during the infection of phytophthora betacei |
| topic | Phytophthora betacei plant–pathogen interaction plant response Solanum betaceum time series analysis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.730251/full |
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