Hadron Therapy, Magnetic Nanoparticles and Hyperthermia: A Promising Combined Tool for Pancreatic Cancer Treatment
A combination of carbon ions/photons irradiation and hyperthermia as a novel therapeutic approach for the in-vitro treatment of pancreatic cancer BxPC3 cells is presented. The radiation doses used are 0–2 Gy for carbon ions and 0–7 Gy for 6 MV photons. Hyperthermia is realized via a standard heating...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/10/1919 |
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| author | Francesca Brero Martin Albino Antonio Antoccia Paolo Arosio Matteo Avolio Francesco Berardinelli Daniela Bettega Paola Calzolari Mario Ciocca Maurizio Corti Angelica Facoetti Salvatore Gallo Flavia Groppi Andrea Guerrini Claudia Innocenti Cristina Lenardi Silvia Locarno Simone Manenti Renato Marchesini Manuel Mariani Francesco Orsini Emanuele Pignoli Claudio Sangregorio Ivan Veronese Alessandro Lascialfari |
| author_facet | Francesca Brero Martin Albino Antonio Antoccia Paolo Arosio Matteo Avolio Francesco Berardinelli Daniela Bettega Paola Calzolari Mario Ciocca Maurizio Corti Angelica Facoetti Salvatore Gallo Flavia Groppi Andrea Guerrini Claudia Innocenti Cristina Lenardi Silvia Locarno Simone Manenti Renato Marchesini Manuel Mariani Francesco Orsini Emanuele Pignoli Claudio Sangregorio Ivan Veronese Alessandro Lascialfari |
| author_sort | Francesca Brero |
| collection | DOAJ |
| description | A combination of carbon ions/photons irradiation and hyperthermia as a novel therapeutic approach for the in-vitro treatment of pancreatic cancer BxPC3 cells is presented. The radiation doses used are 0–2 Gy for carbon ions and 0–7 Gy for 6 MV photons. Hyperthermia is realized via a standard heating bath, assisted by magnetic fluid hyperthermia (MFH) that utilizes magnetic nanoparticles (MNPs) exposed to an alternating magnetic field of amplitude 19.5 mTesla and frequency 109.8 kHz. Starting from 37 °C, the temperature is gradually increased and the sample is kept at 42 °C for 30 min. For MFH, MNPs with a mean diameter of 19 nm and specific absorption rate of 110 ± 30 W/g<sub>Fe3</sub>o<sub>4</sub> coated with a biocompatible ligand to ensure stability in physiological media are used. Irradiation diminishes the clonogenic survival at an extent that depends on the radiation type, and its decrease is amplified both by the MNPs cellular uptake and the hyperthermia protocol. Significant increases in DNA double-strand breaks at 6 h are observed in samples exposed to MNP uptake, treated with 0.75 Gy carbon-ion irradiation and hyperthermia. The proposed experimental protocol, based on the combination of hadron irradiation and hyperthermia, represents a first step towards an innovative clinical option for pancreatic cancer. |
| first_indexed | 2024-03-10T16:02:25Z |
| format | Article |
| id | doaj.art-1a261ad289da48f4880ede0370f3b64a |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T16:02:25Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-1a261ad289da48f4880ede0370f3b64a2023-11-20T15:09:41ZengMDPI AGNanomaterials2079-49912020-09-011010191910.3390/nano10101919Hadron Therapy, Magnetic Nanoparticles and Hyperthermia: A Promising Combined Tool for Pancreatic Cancer TreatmentFrancesca Brero0Martin Albino1Antonio Antoccia2Paolo Arosio3Matteo Avolio4Francesco Berardinelli5Daniela Bettega6Paola Calzolari7Mario Ciocca8Maurizio Corti9Angelica Facoetti10Salvatore Gallo11Flavia Groppi12Andrea Guerrini13Claudia Innocenti14Cristina Lenardi15Silvia Locarno16Simone Manenti17Renato Marchesini18Manuel Mariani19Francesco Orsini20Emanuele Pignoli21Claudio Sangregorio22Ivan Veronese23Alessandro Lascialfari24Dipartimento di Fisica and INFN, Università degli Studi di Pavia, 27100 Pavia, ItalyDipartimento di Chimica, Università di Firenze and INSTM, 50019 Sesto Fiorentino (FI), ItalyDipartimento di Scienze and INFN, Università Roma Tre, 00146 Roma, ItalyDipartimento di Fisica and INFN, Università degli Studi di Milano, 20133 Milano, ItalyDipartimento di Fisica and INFN, Università degli Studi di Pavia, 27100 Pavia, ItalyDipartimento di Scienze and INFN, Università Roma Tre, 00146 Roma, ItalyDipartimento di Fisica and INFN, Università degli Studi di Milano, 20133 Milano, ItalyDipartimento di Fisica and INFN, Università degli Studi di Milano, 20133 Milano, ItalyFondazione CNAO, 27100 Pavia, ItalyDipartimento di Fisica and INFN, Università degli Studi di Pavia, 27100 Pavia, ItalyFondazione CNAO, 27100 Pavia, ItalyDipartimento di Fisica and INFN, Università degli Studi di Milano, 20133 Milano, ItalyDipartimento di Fisica, Università degli Studi di Milano and INFN, Lab. LASA, 20090 Segrate (MI), ItalyDipartimento di Chimica, Università di Firenze and INSTM, 50019 Sesto Fiorentino (FI), ItalyDipartimento di Chimica, Università di Firenze and INSTM, 50019 Sesto Fiorentino (FI), ItalyDipartimento di Fisica and INFN, Università degli Studi di Milano, 20133 Milano, ItalyDipartimento di Fisica and INFN, Università degli Studi di Milano, 20133 Milano, ItalyDipartimento di Fisica, Università degli Studi di Milano and INFN, Lab. LASA, 20090 Segrate (MI), ItalyDipartimento di Fisica and INFN, Università degli Studi di Milano, 20133 Milano, ItalyDipartimento di Fisica and INFN, Università degli Studi di Pavia, 27100 Pavia, ItalyDipartimento di Fisica and INFN, Università degli Studi di Milano, 20133 Milano, ItalyFondazione IRCSS Istituto Nazionale dei tumori, 20133 Milano, ItalyDipartimento di Chimica, Università di Firenze and INSTM, 50019 Sesto Fiorentino (FI), ItalyDipartimento di Fisica and INFN, Università degli Studi di Milano, 20133 Milano, ItalyDipartimento di Fisica and INFN, Università degli Studi di Pavia, 27100 Pavia, ItalyA combination of carbon ions/photons irradiation and hyperthermia as a novel therapeutic approach for the in-vitro treatment of pancreatic cancer BxPC3 cells is presented. The radiation doses used are 0–2 Gy for carbon ions and 0–7 Gy for 6 MV photons. Hyperthermia is realized via a standard heating bath, assisted by magnetic fluid hyperthermia (MFH) that utilizes magnetic nanoparticles (MNPs) exposed to an alternating magnetic field of amplitude 19.5 mTesla and frequency 109.8 kHz. Starting from 37 °C, the temperature is gradually increased and the sample is kept at 42 °C for 30 min. For MFH, MNPs with a mean diameter of 19 nm and specific absorption rate of 110 ± 30 W/g<sub>Fe3</sub>o<sub>4</sub> coated with a biocompatible ligand to ensure stability in physiological media are used. Irradiation diminishes the clonogenic survival at an extent that depends on the radiation type, and its decrease is amplified both by the MNPs cellular uptake and the hyperthermia protocol. Significant increases in DNA double-strand breaks at 6 h are observed in samples exposed to MNP uptake, treated with 0.75 Gy carbon-ion irradiation and hyperthermia. The proposed experimental protocol, based on the combination of hadron irradiation and hyperthermia, represents a first step towards an innovative clinical option for pancreatic cancer.https://www.mdpi.com/2079-4991/10/10/1919hadron therapymagnetic nanoparticleshyperthermiananomaterialsmagnetic fluid hyperthermiapancreatic cancer |
| spellingShingle | Francesca Brero Martin Albino Antonio Antoccia Paolo Arosio Matteo Avolio Francesco Berardinelli Daniela Bettega Paola Calzolari Mario Ciocca Maurizio Corti Angelica Facoetti Salvatore Gallo Flavia Groppi Andrea Guerrini Claudia Innocenti Cristina Lenardi Silvia Locarno Simone Manenti Renato Marchesini Manuel Mariani Francesco Orsini Emanuele Pignoli Claudio Sangregorio Ivan Veronese Alessandro Lascialfari Hadron Therapy, Magnetic Nanoparticles and Hyperthermia: A Promising Combined Tool for Pancreatic Cancer Treatment Nanomaterials hadron therapy magnetic nanoparticles hyperthermia nanomaterials magnetic fluid hyperthermia pancreatic cancer |
| title | Hadron Therapy, Magnetic Nanoparticles and Hyperthermia: A Promising Combined Tool for Pancreatic Cancer Treatment |
| title_full | Hadron Therapy, Magnetic Nanoparticles and Hyperthermia: A Promising Combined Tool for Pancreatic Cancer Treatment |
| title_fullStr | Hadron Therapy, Magnetic Nanoparticles and Hyperthermia: A Promising Combined Tool for Pancreatic Cancer Treatment |
| title_full_unstemmed | Hadron Therapy, Magnetic Nanoparticles and Hyperthermia: A Promising Combined Tool for Pancreatic Cancer Treatment |
| title_short | Hadron Therapy, Magnetic Nanoparticles and Hyperthermia: A Promising Combined Tool for Pancreatic Cancer Treatment |
| title_sort | hadron therapy magnetic nanoparticles and hyperthermia a promising combined tool for pancreatic cancer treatment |
| topic | hadron therapy magnetic nanoparticles hyperthermia nanomaterials magnetic fluid hyperthermia pancreatic cancer |
| url | https://www.mdpi.com/2079-4991/10/10/1919 |
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