Hormonome Dynamics During Microgametogenesis in Different Nicotiana Species
Plant microgametogenesis involves stages leading to the progressive development of unicellular microspores into mature pollen. Despite the active and continuing interest in the study of male reproductive development, little is still known about the hormonomics at each ontogenetic stage. In this work...
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.735451/full |
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| author | Lenka Záveská Drábková Eva Pokorná Petre I. Dobrev Jana Kůrková Lenka Steinbachová David Honys Václav Motyka |
| author_facet | Lenka Záveská Drábková Eva Pokorná Petre I. Dobrev Jana Kůrková Lenka Steinbachová David Honys Václav Motyka |
| author_sort | Lenka Záveská Drábková |
| collection | DOAJ |
| description | Plant microgametogenesis involves stages leading to the progressive development of unicellular microspores into mature pollen. Despite the active and continuing interest in the study of male reproductive development, little is still known about the hormonomics at each ontogenetic stage. In this work, we characterized the profiles and dynamics of phytohormones during the process of microgametogenesis in four Nicotiana species (Nicotiana tabacum, Nicotiana alata, Nicotiana langsdorffii, and Nicotiana mutabilis). Taking advantage of advanced HPLC-ESI-MS/MS, twenty to thirty endogenous hormone derivatives were identified throughout pollen ontogenesis, including cytokinins, auxins, ABA and its derivatives, jasmonates, and phenolic compounds. The spectra of endogenous phytohormones changed dynamically during tobacco pollen ontogeny, indicating their important role in pollen growth and development. The different dynamics in the accumulation of endogenous phytohormones during pollen ontogenesis between N. tabacum (section Nicotiana) and the other three species (section Alatae) reflects their different phylogenetic positions and origin within the genus Nicotiana. We demonstrated the involvement of certain phytohormone forms, such as cis-zeatin- and methylthiol-type CKs, some derivatives of abscisic acid, phenylacetic and benzoic acids, in pollen development for the first time here. Our results suggest that unequal levels of endogenous hormones and the presence of specific derivatives may be characteristic for pollen development in different phylogenetic plant groups. These results represent the currently most comprehensive study of plant hormones during the process of pollen development. |
| first_indexed | 2024-12-19T15:32:20Z |
| format | Article |
| id | doaj.art-1a267c903f724c1c846bf527ece728dc |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T15:32:20Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1a267c903f724c1c846bf527ece728dc2022-12-21T20:15:41ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-10-011210.3389/fpls.2021.735451735451Hormonome Dynamics During Microgametogenesis in Different Nicotiana SpeciesLenka Záveská Drábková0Eva Pokorná1Petre I. Dobrev2Jana Kůrková3Lenka Steinbachová4David Honys5Václav Motyka6Laboratory of Pollen Biology, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Hormonal Regulations in Plants, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Hormonal Regulations in Plants, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Pollen Biology, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Pollen Biology, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Pollen Biology, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Hormonal Regulations in Plants, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, CzechiaPlant microgametogenesis involves stages leading to the progressive development of unicellular microspores into mature pollen. Despite the active and continuing interest in the study of male reproductive development, little is still known about the hormonomics at each ontogenetic stage. In this work, we characterized the profiles and dynamics of phytohormones during the process of microgametogenesis in four Nicotiana species (Nicotiana tabacum, Nicotiana alata, Nicotiana langsdorffii, and Nicotiana mutabilis). Taking advantage of advanced HPLC-ESI-MS/MS, twenty to thirty endogenous hormone derivatives were identified throughout pollen ontogenesis, including cytokinins, auxins, ABA and its derivatives, jasmonates, and phenolic compounds. The spectra of endogenous phytohormones changed dynamically during tobacco pollen ontogeny, indicating their important role in pollen growth and development. The different dynamics in the accumulation of endogenous phytohormones during pollen ontogenesis between N. tabacum (section Nicotiana) and the other three species (section Alatae) reflects their different phylogenetic positions and origin within the genus Nicotiana. We demonstrated the involvement of certain phytohormone forms, such as cis-zeatin- and methylthiol-type CKs, some derivatives of abscisic acid, phenylacetic and benzoic acids, in pollen development for the first time here. Our results suggest that unequal levels of endogenous hormones and the presence of specific derivatives may be characteristic for pollen development in different phylogenetic plant groups. These results represent the currently most comprehensive study of plant hormones during the process of pollen development.https://www.frontiersin.org/articles/10.3389/fpls.2021.735451/fullhormonomemale gametophyteNicotiana spp.ontogenypollen developmentphytohormones |
| spellingShingle | Lenka Záveská Drábková Eva Pokorná Petre I. Dobrev Jana Kůrková Lenka Steinbachová David Honys Václav Motyka Hormonome Dynamics During Microgametogenesis in Different Nicotiana Species Frontiers in Plant Science hormonome male gametophyte Nicotiana spp. ontogeny pollen development phytohormones |
| title | Hormonome Dynamics During Microgametogenesis in Different Nicotiana Species |
| title_full | Hormonome Dynamics During Microgametogenesis in Different Nicotiana Species |
| title_fullStr | Hormonome Dynamics During Microgametogenesis in Different Nicotiana Species |
| title_full_unstemmed | Hormonome Dynamics During Microgametogenesis in Different Nicotiana Species |
| title_short | Hormonome Dynamics During Microgametogenesis in Different Nicotiana Species |
| title_sort | hormonome dynamics during microgametogenesis in different nicotiana species |
| topic | hormonome male gametophyte Nicotiana spp. ontogeny pollen development phytohormones |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.735451/full |
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