Korean red ginseng extract prevents bone loss in an oral model of glucocorticoid induced osteoporosis in mice
The gut microbiota and barrier function play important roles in bone health. We previously demonstrated that chronic glucocorticoid (GC)-induced bone loss in mice is associated with significant shifts in gut microbiota composition and impaired gut barrier function. Korean Red Ginseng (KRG, Panax Gin...
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| Format: | Article |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1268134/full |
| _version_ | 1797265442952183808 |
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| author | Nicholas J. Chargo Nicholas J. Chargo Ho Jun Kang Subhashari Das Yining Jin Cheryl Rockwell Jae Youl Cho Laura R. McCabe Laura R. McCabe Narayanan Parameswaran Narayanan Parameswaran |
| author_facet | Nicholas J. Chargo Nicholas J. Chargo Ho Jun Kang Subhashari Das Yining Jin Cheryl Rockwell Jae Youl Cho Laura R. McCabe Laura R. McCabe Narayanan Parameswaran Narayanan Parameswaran |
| author_sort | Nicholas J. Chargo |
| collection | DOAJ |
| description | The gut microbiota and barrier function play important roles in bone health. We previously demonstrated that chronic glucocorticoid (GC)-induced bone loss in mice is associated with significant shifts in gut microbiota composition and impaired gut barrier function. Korean Red Ginseng (KRG, Panax Ginseng Meyer, Araliaceae) extract has been shown to prevent glucocorticoid-induced osteoporosis (GIO) in a subcutaneous pellet model in mice, but its effect on gut microbiota and barrier function in this context is not known. The overall goal of this study was to test the effect of KRG extract in a clinically relevant, oral model of GIO and further investigate its role in modulating the gut-bone axis. Growing male mice (CD-1, 8 weeks) were treated with 75 μg/mL corticosterone (∼9 mg/kg/day) or 0.4% ethanol vehicle in the drinking water for 4 weeks. During this 4-week period, mice were treated daily with 500 mg/kg/day KRG extract dissolved in sterile water or an equal amount of sterile water via oral gastric gavage. After 4 weeks of treatment, we assessed bone volume, microbiota composition, gut barrier integrity, and immune cells in the bone marrow (BM) and mesenteric lymph nodes (MLNs). 4 weeks of oral GC treatment caused significant distal femur trabecular bone loss, and this was associated with changes in gut microbiota composition, impaired gut barrier function and altered immune cell composition. Importantly, KRG extract prevented distal femur trabecular bone loss and caused significant alterations in gut microbiota composition but had only modest effects on gut barrier function and immune cell populations. Taken together, these results demonstrate that KRG extract significantly modulates the gut microbiota-bone axis and prevents glucocorticoid-induced bone loss in mice. |
| first_indexed | 2024-04-25T00:44:52Z |
| format | Article |
| id | doaj.art-1a27a5873df940dda961212053f16b7a |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-25T00:44:52Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-1a27a5873df940dda961212053f16b7a2024-03-12T04:58:06ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122024-03-011510.3389/fphar.2024.12681341268134Korean red ginseng extract prevents bone loss in an oral model of glucocorticoid induced osteoporosis in miceNicholas J. Chargo0Nicholas J. Chargo1Ho Jun Kang2Subhashari Das3Yining Jin4Cheryl Rockwell5Jae Youl Cho6Laura R. McCabe7Laura R. McCabe8Narayanan Parameswaran9Narayanan Parameswaran10Department of Physiology, Michigan State University, East Lansing, MI, United StatesCollege of Osteopathic Medicine, Michigan State University, East Lansing, MI, United StatesDepartment of Physiology, Michigan State University, East Lansing, MI, United StatesDepartment of Physiology, Michigan State University, East Lansing, MI, United StatesDepartment of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, United StatesDepartment of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, United StatesDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, Republic of KoreaDepartment of Physiology, Michigan State University, East Lansing, MI, United StatesCollege of Osteopathic Medicine, Michigan State University, East Lansing, MI, United StatesDepartment of Physiology, Michigan State University, East Lansing, MI, United StatesCollege of Human Medicine, Michigan State University, East Lansing, MI, United StatesThe gut microbiota and barrier function play important roles in bone health. We previously demonstrated that chronic glucocorticoid (GC)-induced bone loss in mice is associated with significant shifts in gut microbiota composition and impaired gut barrier function. Korean Red Ginseng (KRG, Panax Ginseng Meyer, Araliaceae) extract has been shown to prevent glucocorticoid-induced osteoporosis (GIO) in a subcutaneous pellet model in mice, but its effect on gut microbiota and barrier function in this context is not known. The overall goal of this study was to test the effect of KRG extract in a clinically relevant, oral model of GIO and further investigate its role in modulating the gut-bone axis. Growing male mice (CD-1, 8 weeks) were treated with 75 μg/mL corticosterone (∼9 mg/kg/day) or 0.4% ethanol vehicle in the drinking water for 4 weeks. During this 4-week period, mice were treated daily with 500 mg/kg/day KRG extract dissolved in sterile water or an equal amount of sterile water via oral gastric gavage. After 4 weeks of treatment, we assessed bone volume, microbiota composition, gut barrier integrity, and immune cells in the bone marrow (BM) and mesenteric lymph nodes (MLNs). 4 weeks of oral GC treatment caused significant distal femur trabecular bone loss, and this was associated with changes in gut microbiota composition, impaired gut barrier function and altered immune cell composition. Importantly, KRG extract prevented distal femur trabecular bone loss and caused significant alterations in gut microbiota composition but had only modest effects on gut barrier function and immune cell populations. Taken together, these results demonstrate that KRG extract significantly modulates the gut microbiota-bone axis and prevents glucocorticoid-induced bone loss in mice.https://www.frontiersin.org/articles/10.3389/fphar.2024.1268134/fullKorean red ginseng extractglucocorticoidbonemicrobiotagut-bone axisgut barrier |
| spellingShingle | Nicholas J. Chargo Nicholas J. Chargo Ho Jun Kang Subhashari Das Yining Jin Cheryl Rockwell Jae Youl Cho Laura R. McCabe Laura R. McCabe Narayanan Parameswaran Narayanan Parameswaran Korean red ginseng extract prevents bone loss in an oral model of glucocorticoid induced osteoporosis in mice Frontiers in Pharmacology Korean red ginseng extract glucocorticoid bone microbiota gut-bone axis gut barrier |
| title | Korean red ginseng extract prevents bone loss in an oral model of glucocorticoid induced osteoporosis in mice |
| title_full | Korean red ginseng extract prevents bone loss in an oral model of glucocorticoid induced osteoporosis in mice |
| title_fullStr | Korean red ginseng extract prevents bone loss in an oral model of glucocorticoid induced osteoporosis in mice |
| title_full_unstemmed | Korean red ginseng extract prevents bone loss in an oral model of glucocorticoid induced osteoporosis in mice |
| title_short | Korean red ginseng extract prevents bone loss in an oral model of glucocorticoid induced osteoporosis in mice |
| title_sort | korean red ginseng extract prevents bone loss in an oral model of glucocorticoid induced osteoporosis in mice |
| topic | Korean red ginseng extract glucocorticoid bone microbiota gut-bone axis gut barrier |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1268134/full |
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