Yeast Functional Screen to Determine cDNAs of European Alkali Grass (Puccinellia distans) Conferring Selenium (Se) Toxicity
Selenium (Se), a metalloid, is essential for humans and animals, but its excess causes toxicity in all living organisms, including plants. Studies have shown that some wild plants such as European alkali grass (P. distans) are much more resistant to metal / metalloid toxicity than crop plants are....
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| Format: | Article |
| Language: | English |
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Institute of Economic Develoment and Social Researches
2022-12-01
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| Series: | ISPEC Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://www.ispecjournal.com/index.php/ispecjas/article/view/366 |
| _version_ | 1826537699973005312 |
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| author | Kübra BUDAK M. Aydın AKBUDAK |
| author_facet | Kübra BUDAK M. Aydın AKBUDAK |
| author_sort | Kübra BUDAK |
| collection | DOAJ |
| description |
Selenium (Se), a metalloid, is essential for humans and animals, but its excess causes toxicity in all living organisms, including plants. Studies have shown that some wild plants such as European alkali grass (P. distans) are much more resistant to metal / metalloid toxicity than crop plants are. Identification of related genes in such resistant plants is very important to solve the problems caused by heavy metal toxicity especially. In the present study, the cDNA libraries were prepared from the roots and leaves of P. distans and transformed into yeast (Saccharomyces cerevisiae) cells to be tested against Se stress. The yeast cells were grown on medium containing a toxic dose of sodium selenite (15 mM), and the resistant colonies were determined. The cDNA-induced resistance in the selected colonies was also confirmed by re-transformation and drop tests. The analyzes showed that obtained from roots and leaves, the cDNAs conferring resistance to yeast cells were different from each other. The findings of this study should provide a useful scientific basis for future studies concerning investigation of genes conferring resistance to metal / metaloid toxicity and revealing the resistance mechanism.
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| first_indexed | 2025-03-14T03:14:52Z |
| format | Article |
| id | doaj.art-1a285ab334154660940be6099ad8c14f |
| institution | Directory Open Access Journal |
| issn | 2717-7238 |
| language | English |
| last_indexed | 2025-03-14T03:14:52Z |
| publishDate | 2022-12-01 |
| publisher | Institute of Economic Develoment and Social Researches |
| record_format | Article |
| series | ISPEC Journal of Agricultural Sciences |
| spelling | doaj.art-1a285ab334154660940be6099ad8c14f2025-03-10T01:31:44ZengInstitute of Economic Develoment and Social ResearchesISPEC Journal of Agricultural Sciences2717-72382022-12-016410.5281/zenodo.7309543Yeast Functional Screen to Determine cDNAs of European Alkali Grass (Puccinellia distans) Conferring Selenium (Se) ToxicityKübra BUDAK0M. Aydın AKBUDAK1Akdeniz Üniversitesi, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü AntalyaAkdeniz Üniversitesi, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü Antalya Selenium (Se), a metalloid, is essential for humans and animals, but its excess causes toxicity in all living organisms, including plants. Studies have shown that some wild plants such as European alkali grass (P. distans) are much more resistant to metal / metalloid toxicity than crop plants are. Identification of related genes in such resistant plants is very important to solve the problems caused by heavy metal toxicity especially. In the present study, the cDNA libraries were prepared from the roots and leaves of P. distans and transformed into yeast (Saccharomyces cerevisiae) cells to be tested against Se stress. The yeast cells were grown on medium containing a toxic dose of sodium selenite (15 mM), and the resistant colonies were determined. The cDNA-induced resistance in the selected colonies was also confirmed by re-transformation and drop tests. The analyzes showed that obtained from roots and leaves, the cDNAs conferring resistance to yeast cells were different from each other. The findings of this study should provide a useful scientific basis for future studies concerning investigation of genes conferring resistance to metal / metaloid toxicity and revealing the resistance mechanism. https://www.ispecjournal.com/index.php/ispecjas/article/view/366Puccinellia distansselenium toxicitycDNAyeast functional screen |
| spellingShingle | Kübra BUDAK M. Aydın AKBUDAK Yeast Functional Screen to Determine cDNAs of European Alkali Grass (Puccinellia distans) Conferring Selenium (Se) Toxicity ISPEC Journal of Agricultural Sciences Puccinellia distans selenium toxicity cDNA yeast functional screen |
| title | Yeast Functional Screen to Determine cDNAs of European Alkali Grass (Puccinellia distans) Conferring Selenium (Se) Toxicity |
| title_full | Yeast Functional Screen to Determine cDNAs of European Alkali Grass (Puccinellia distans) Conferring Selenium (Se) Toxicity |
| title_fullStr | Yeast Functional Screen to Determine cDNAs of European Alkali Grass (Puccinellia distans) Conferring Selenium (Se) Toxicity |
| title_full_unstemmed | Yeast Functional Screen to Determine cDNAs of European Alkali Grass (Puccinellia distans) Conferring Selenium (Se) Toxicity |
| title_short | Yeast Functional Screen to Determine cDNAs of European Alkali Grass (Puccinellia distans) Conferring Selenium (Se) Toxicity |
| title_sort | yeast functional screen to determine cdnas of european alkali grass puccinellia distans conferring selenium se toxicity |
| topic | Puccinellia distans selenium toxicity cDNA yeast functional screen |
| url | https://www.ispecjournal.com/index.php/ispecjas/article/view/366 |
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