Adeno-associated virus type 2 (AAV2) uncoating is a stepwise process and is linked to structural reorganization of the nucleolus.
Nucleoli are membrane-less structures located within the nucleus and are known to be involved in many cellular functions, including stress response and cell cycle regulation. Besides, many viruses can employ the nucleolus or nucleolar proteins to promote different steps of their life cycle such as r...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-07-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1010187 |
| _version_ | 1811214701329121280 |
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| author | Sereina O Sutter Anouk Lkharrazi Elisabeth M Schraner Kevin Michaelsen Anita Felicitas Meier Jennifer Marx Bernd Vogt Hildegard Büning Cornel Fraefel |
| author_facet | Sereina O Sutter Anouk Lkharrazi Elisabeth M Schraner Kevin Michaelsen Anita Felicitas Meier Jennifer Marx Bernd Vogt Hildegard Büning Cornel Fraefel |
| author_sort | Sereina O Sutter |
| collection | DOAJ |
| description | Nucleoli are membrane-less structures located within the nucleus and are known to be involved in many cellular functions, including stress response and cell cycle regulation. Besides, many viruses can employ the nucleolus or nucleolar proteins to promote different steps of their life cycle such as replication, transcription and assembly. While adeno-associated virus type 2 (AAV2) capsids have previously been reported to enter the host cell nucleus and accumulate in the nucleolus, both the role of the nucleolus in AAV2 infection, and the viral uncoating mechanism remain elusive. In all prior studies on AAV uncoating, viral capsids and viral genomes were not directly correlated on the single cell level, at least not in absence of a helper virus. To elucidate the properties of the nucleolus during AAV2 infection and to assess viral uncoating on a single cell level, we combined immunofluorescence analysis for detection of intact AAV2 capsids and capsid proteins with fluorescence in situ hybridization for detection of AAV2 genomes. The results of our experiments provide evidence that uncoating of AAV2 particles occurs in a stepwise process that is completed in the nucleolus and supported by alteration of the nucleolar structure. |
| first_indexed | 2024-04-12T06:09:07Z |
| format | Article |
| id | doaj.art-1a2d5a0947f944dcb06d6d884bcbc3df |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-12T06:09:07Z |
| publishDate | 2022-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-1a2d5a0947f944dcb06d6d884bcbc3df2022-12-22T03:44:46ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742022-07-01187e101018710.1371/journal.ppat.1010187Adeno-associated virus type 2 (AAV2) uncoating is a stepwise process and is linked to structural reorganization of the nucleolus.Sereina O SutterAnouk LkharraziElisabeth M SchranerKevin MichaelsenAnita Felicitas MeierJennifer MarxBernd VogtHildegard BüningCornel FraefelNucleoli are membrane-less structures located within the nucleus and are known to be involved in many cellular functions, including stress response and cell cycle regulation. Besides, many viruses can employ the nucleolus or nucleolar proteins to promote different steps of their life cycle such as replication, transcription and assembly. While adeno-associated virus type 2 (AAV2) capsids have previously been reported to enter the host cell nucleus and accumulate in the nucleolus, both the role of the nucleolus in AAV2 infection, and the viral uncoating mechanism remain elusive. In all prior studies on AAV uncoating, viral capsids and viral genomes were not directly correlated on the single cell level, at least not in absence of a helper virus. To elucidate the properties of the nucleolus during AAV2 infection and to assess viral uncoating on a single cell level, we combined immunofluorescence analysis for detection of intact AAV2 capsids and capsid proteins with fluorescence in situ hybridization for detection of AAV2 genomes. The results of our experiments provide evidence that uncoating of AAV2 particles occurs in a stepwise process that is completed in the nucleolus and supported by alteration of the nucleolar structure.https://doi.org/10.1371/journal.ppat.1010187 |
| spellingShingle | Sereina O Sutter Anouk Lkharrazi Elisabeth M Schraner Kevin Michaelsen Anita Felicitas Meier Jennifer Marx Bernd Vogt Hildegard Büning Cornel Fraefel Adeno-associated virus type 2 (AAV2) uncoating is a stepwise process and is linked to structural reorganization of the nucleolus. PLoS Pathogens |
| title | Adeno-associated virus type 2 (AAV2) uncoating is a stepwise process and is linked to structural reorganization of the nucleolus. |
| title_full | Adeno-associated virus type 2 (AAV2) uncoating is a stepwise process and is linked to structural reorganization of the nucleolus. |
| title_fullStr | Adeno-associated virus type 2 (AAV2) uncoating is a stepwise process and is linked to structural reorganization of the nucleolus. |
| title_full_unstemmed | Adeno-associated virus type 2 (AAV2) uncoating is a stepwise process and is linked to structural reorganization of the nucleolus. |
| title_short | Adeno-associated virus type 2 (AAV2) uncoating is a stepwise process and is linked to structural reorganization of the nucleolus. |
| title_sort | adeno associated virus type 2 aav2 uncoating is a stepwise process and is linked to structural reorganization of the nucleolus |
| url | https://doi.org/10.1371/journal.ppat.1010187 |
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