Combinatorial Pharyngeal Taste Coding for Feeding Avoidance in Adult Drosophila
Summary: Taste drives appropriate food preference and intake. In Drosophila, taste neurons are housed in both external and internal organs, but the latter have been relatively underexplored. Here, we report that Poxn mutants with a minimal taste system of pharyngeal neurons can avoid many aversive t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-10-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124719312240 |
| _version_ | 1818019081525133312 |
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| author | Yu-Chieh David Chen Scarlet Jinhong Park Ryan Matthew Joseph William W. Ja Anupama Arun Dahanukar |
| author_facet | Yu-Chieh David Chen Scarlet Jinhong Park Ryan Matthew Joseph William W. Ja Anupama Arun Dahanukar |
| author_sort | Yu-Chieh David Chen |
| collection | DOAJ |
| description | Summary: Taste drives appropriate food preference and intake. In Drosophila, taste neurons are housed in both external and internal organs, but the latter have been relatively underexplored. Here, we report that Poxn mutants with a minimal taste system of pharyngeal neurons can avoid many aversive tastants, including bitter compounds, acid, and salt, suggesting that pharyngeal taste is sufficient for rejecting intake of aversive compounds. Optogenetic activation of selected pharyngeal bitter neurons during feeding events elicits changes in feeding parameters that can suppress intake. Functional dissection experiments indicate that multiple classes of pharyngeal neurons are involved in achieving behavioral avoidance, by virtue of being inhibited or activated by aversive tastants. Tracing second-order pharyngeal circuits reveals two main relay centers for processing pharyngeal taste inputs. Together, our results suggest that the pharynx can control the ingestion of harmful compounds by integrating taste input from different classes of pharyngeal neurons. : Chen et al. perform functional and behavioral experiments to study the roles of different subsets of pharyngeal neurons in governing food avoidance in flies. They find evidence that rejection of different categories of aversive compounds is dependent on distinct combinations of pharyngeal taste neurons. Keywords: Drosophila, taste, gustation, pharynx, aversive compounds, feeding avoidance, trans-Tango mapping |
| first_indexed | 2024-04-14T07:46:47Z |
| format | Article |
| id | doaj.art-1a2d8af3f5d1434bb89077133c65fa68 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-14T07:46:47Z |
| publishDate | 2019-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-1a2d8af3f5d1434bb89077133c65fa682022-12-22T02:05:18ZengElsevierCell Reports2211-12472019-10-01294961973.e4Combinatorial Pharyngeal Taste Coding for Feeding Avoidance in Adult DrosophilaYu-Chieh David Chen0Scarlet Jinhong Park1Ryan Matthew Joseph2William W. Ja3Anupama Arun Dahanukar4Interdepartmental Neuroscience Program, University of California, Riverside, CA 92521, USADepartment of Neuroscience, The Scripps Research Institute, Jupiter, FL 33458, USA; Skaggs Graduate School, The Scripps Research Institute, Jupiter, FL 33458, USADepartment of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, USADepartment of Neuroscience, The Scripps Research Institute, Jupiter, FL 33458, USA; Skaggs Graduate School, The Scripps Research Institute, Jupiter, FL 33458, USAInterdepartmental Neuroscience Program, University of California, Riverside, CA 92521, USA; Department of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, USA; Corresponding authorSummary: Taste drives appropriate food preference and intake. In Drosophila, taste neurons are housed in both external and internal organs, but the latter have been relatively underexplored. Here, we report that Poxn mutants with a minimal taste system of pharyngeal neurons can avoid many aversive tastants, including bitter compounds, acid, and salt, suggesting that pharyngeal taste is sufficient for rejecting intake of aversive compounds. Optogenetic activation of selected pharyngeal bitter neurons during feeding events elicits changes in feeding parameters that can suppress intake. Functional dissection experiments indicate that multiple classes of pharyngeal neurons are involved in achieving behavioral avoidance, by virtue of being inhibited or activated by aversive tastants. Tracing second-order pharyngeal circuits reveals two main relay centers for processing pharyngeal taste inputs. Together, our results suggest that the pharynx can control the ingestion of harmful compounds by integrating taste input from different classes of pharyngeal neurons. : Chen et al. perform functional and behavioral experiments to study the roles of different subsets of pharyngeal neurons in governing food avoidance in flies. They find evidence that rejection of different categories of aversive compounds is dependent on distinct combinations of pharyngeal taste neurons. Keywords: Drosophila, taste, gustation, pharynx, aversive compounds, feeding avoidance, trans-Tango mappinghttp://www.sciencedirect.com/science/article/pii/S2211124719312240 |
| spellingShingle | Yu-Chieh David Chen Scarlet Jinhong Park Ryan Matthew Joseph William W. Ja Anupama Arun Dahanukar Combinatorial Pharyngeal Taste Coding for Feeding Avoidance in Adult Drosophila Cell Reports |
| title | Combinatorial Pharyngeal Taste Coding for Feeding Avoidance in Adult Drosophila |
| title_full | Combinatorial Pharyngeal Taste Coding for Feeding Avoidance in Adult Drosophila |
| title_fullStr | Combinatorial Pharyngeal Taste Coding for Feeding Avoidance in Adult Drosophila |
| title_full_unstemmed | Combinatorial Pharyngeal Taste Coding for Feeding Avoidance in Adult Drosophila |
| title_short | Combinatorial Pharyngeal Taste Coding for Feeding Avoidance in Adult Drosophila |
| title_sort | combinatorial pharyngeal taste coding for feeding avoidance in adult drosophila |
| url | http://www.sciencedirect.com/science/article/pii/S2211124719312240 |
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