MRTF-A Regulates the Proliferation and Migration of Non-small Cell Lung Cancer Cells of A549 through HOTAIR
Background and objective Non-small cell lung cancer (NSCLC) is a kind of lung cancer, because its high incidence has been concerned. Therefore, it has great significance to reveal the pathogenesis of NSCLC. As a transcriptional regulatory factor, MATF-A plays an important role in the development of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2019-02-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2019.02.02 |
| _version_ | 1818337034800988160 |
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| author | Kun ZHANG Yubin ZHOU Gang FENG Fuchun ZENG |
| author_facet | Kun ZHANG Yubin ZHOU Gang FENG Fuchun ZENG |
| author_sort | Kun ZHANG |
| collection | DOAJ |
| description | Background and objective Non-small cell lung cancer (NSCLC) is a kind of lung cancer, because its high incidence has been concerned. Therefore, it has great significance to reveal the pathogenesis of NSCLC. As a transcriptional regulatory factor, MATF-A plays an important role in the development of multiple tumors, can regulate the migration process of a variety of tumor cells. HOTAIR is a long non-coding RNA (LncRNA) found in recent years, which expresses abnormally in multiple tumors and is involved in the proliferation and migration of multiple tumors. The aim of this study is to explore the role of MRTF-A through HOTAIR to regulate the proliferation and migration of NSCLC cell A549 cell. Methods We constructed the overexpression plasmid and interfering plasmid of MRTF-A, and detected the effect of MRTF-A on the proliferation and migration of A549 cells by CCK8 and wound healing methods respectively. Then, we designed the siRNA of HOTAIR to detect its effect on the proliferation and migration of A549 cells. Through qRT-PCR, we detected the effect of MRTF-A on HOTAIR expression. Finally, we constructed HOTAIR's promoter, and detect the effect of MRTF-A on HOTAIR promoter activity by luciferase reporter gene test. Results Overexpression of MRTF-A promotes the proliferation and migration of A549 cells, while silent MRTF-A inhibits its proliferation and migration. Next, we found that interfered HOTAIR expression inhibited the proliferation of A549 cells. We found that MRTF-A could influence the expression of HOTAIR and regulate the activity of HOTAIR promoter. Conclusion MRTF-A regulates the proliferation and migration of A549 cell through HOTAIR. |
| first_indexed | 2024-12-13T14:48:48Z |
| format | Article |
| id | doaj.art-1a341d3426a34985ba9f1481c48f6515 |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-12-13T14:48:48Z |
| publishDate | 2019-02-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-1a341d3426a34985ba9f1481c48f65152022-12-21T23:41:23ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872019-02-01222828910.3779/j.issn.1009-3419.2019.02.02MRTF-A Regulates the Proliferation and Migration of Non-small Cell Lung Cancer Cells of A549 through HOTAIRKun ZHANG0Yubin ZHOU1Gang FENG2Fuchun ZENG3Department of Thoracic Surgery, Sichuan Provincial People's Hospital, Chengdu 610072, ChinaDepartment of Thoracic Surgery, Sichuan Provincial People's Hospital, Chengdu 610072, ChinaDepartment of Thoracic Surgery, Sichuan Provincial People's Hospital, Chengdu 610072, ChinaDepartment of Thoracic Surgery, Sichuan Provincial People's Hospital, Chengdu 610072, ChinaBackground and objective Non-small cell lung cancer (NSCLC) is a kind of lung cancer, because its high incidence has been concerned. Therefore, it has great significance to reveal the pathogenesis of NSCLC. As a transcriptional regulatory factor, MATF-A plays an important role in the development of multiple tumors, can regulate the migration process of a variety of tumor cells. HOTAIR is a long non-coding RNA (LncRNA) found in recent years, which expresses abnormally in multiple tumors and is involved in the proliferation and migration of multiple tumors. The aim of this study is to explore the role of MRTF-A through HOTAIR to regulate the proliferation and migration of NSCLC cell A549 cell. Methods We constructed the overexpression plasmid and interfering plasmid of MRTF-A, and detected the effect of MRTF-A on the proliferation and migration of A549 cells by CCK8 and wound healing methods respectively. Then, we designed the siRNA of HOTAIR to detect its effect on the proliferation and migration of A549 cells. Through qRT-PCR, we detected the effect of MRTF-A on HOTAIR expression. Finally, we constructed HOTAIR's promoter, and detect the effect of MRTF-A on HOTAIR promoter activity by luciferase reporter gene test. Results Overexpression of MRTF-A promotes the proliferation and migration of A549 cells, while silent MRTF-A inhibits its proliferation and migration. Next, we found that interfered HOTAIR expression inhibited the proliferation of A549 cells. We found that MRTF-A could influence the expression of HOTAIR and regulate the activity of HOTAIR promoter. Conclusion MRTF-A regulates the proliferation and migration of A549 cell through HOTAIR.http://dx.doi.org/10.3779/j.issn.1009-3419.2019.02.02Lung neoplasmsA549MRTF-AHOTAIRProliferationMigration |
| spellingShingle | Kun ZHANG Yubin ZHOU Gang FENG Fuchun ZENG MRTF-A Regulates the Proliferation and Migration of Non-small Cell Lung Cancer Cells of A549 through HOTAIR Chinese Journal of Lung Cancer Lung neoplasms A549 MRTF-A HOTAIR Proliferation Migration |
| title | MRTF-A Regulates the Proliferation and Migration of Non-small Cell Lung Cancer Cells of A549 through HOTAIR |
| title_full | MRTF-A Regulates the Proliferation and Migration of Non-small Cell Lung Cancer Cells of A549 through HOTAIR |
| title_fullStr | MRTF-A Regulates the Proliferation and Migration of Non-small Cell Lung Cancer Cells of A549 through HOTAIR |
| title_full_unstemmed | MRTF-A Regulates the Proliferation and Migration of Non-small Cell Lung Cancer Cells of A549 through HOTAIR |
| title_short | MRTF-A Regulates the Proliferation and Migration of Non-small Cell Lung Cancer Cells of A549 through HOTAIR |
| title_sort | mrtf a regulates the proliferation and migration of non small cell lung cancer cells of a549 through hotair |
| topic | Lung neoplasms A549 MRTF-A HOTAIR Proliferation Migration |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2019.02.02 |
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