Production and Characterization of the Beneficial β-glucuronidase Inhibitor from non pathogenic wild yeast, Candida oleophila WP5-19-1, and its effects on gut microbes
We aimed to produce a potent β-glucuronidase inhibitor from wild yeast that could inactivate toxic substances in the colon. Culture supernatants and cell-free extracts of non-pathogenic wild yeasts were prepared and their β-glucuronidase inhibitory activities were measured. Cell-free extract from Ca...
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| Format: | Article |
| Language: | English |
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The Korean Society of Mycology
2022-03-01
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| Series: | 한국균학회지 |
| Subjects: | |
| Online Access: | http://www.kjmycology.or.kr/article/?num=N0320500102 |
| _version_ | 1818490280434728960 |
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| author | Seon-Jeong Park Jong-Soo Lee |
| author_facet | Seon-Jeong Park Jong-Soo Lee |
| author_sort | Seon-Jeong Park |
| collection | DOAJ |
| description | We aimed to produce a potent β-glucuronidase inhibitor from wild yeast that could inactivate toxic substances in the colon. Culture supernatants and cell-free extracts of non-pathogenic wild yeasts were prepared and their β-glucuronidase inhibitory activities were measured. Cell-free extract from Candida oleophila WP5-19-1 showed the highest β-glucuronidase inhibitory activity (49.0%). The β-glucuronidase inhibitor was maximally produced (IC50 value; 8.4 mg) when C. oleophila WP5-19-1 was cultured in potato dextrose medium containing 5% dextrose (initial pH; 6.0) at 30℃ for 24 hours. β-glucuronidase inhibitor of C. oleophila WP5-19-1 was partially purified by trypsin hydrolysis, ultrafiltration (3 kDa), and Sephadex G-50 filtration. The partially purified β-glucuronidase inhibitor was stable from 30℃ to 60℃ and at pH 6.0 9.0, and showed residual inhibitory activity of about 80%. |
| first_indexed | 2024-12-10T17:14:36Z |
| format | Article |
| id | doaj.art-1a34422c8c884001906812ddc3f7001e |
| institution | Directory Open Access Journal |
| issn | 0253-651X 2383-5249 |
| language | English |
| last_indexed | 2024-12-10T17:14:36Z |
| publishDate | 2022-03-01 |
| publisher | The Korean Society of Mycology |
| record_format | Article |
| series | 한국균학회지 |
| spelling | doaj.art-1a34422c8c884001906812ddc3f7001e2022-12-22T01:40:11ZengThe Korean Society of Mycology한국균학회지0253-651X2383-52492022-03-01501314010.4489/KJM.20220002N0320500102Production and Characterization of the Beneficial β-glucuronidase Inhibitor from non pathogenic wild yeast, Candida oleophila WP5-19-1, and its effects on gut microbesSeon-Jeong Park0Jong-Soo Lee1Department of Biomedicine and Biotechnology, Paichai University, Daejeon 35345, Korea.Department of Biomedicine and Biotechnology, Paichai University, Daejeon 35345, Korea.We aimed to produce a potent β-glucuronidase inhibitor from wild yeast that could inactivate toxic substances in the colon. Culture supernatants and cell-free extracts of non-pathogenic wild yeasts were prepared and their β-glucuronidase inhibitory activities were measured. Cell-free extract from Candida oleophila WP5-19-1 showed the highest β-glucuronidase inhibitory activity (49.0%). The β-glucuronidase inhibitor was maximally produced (IC50 value; 8.4 mg) when C. oleophila WP5-19-1 was cultured in potato dextrose medium containing 5% dextrose (initial pH; 6.0) at 30℃ for 24 hours. β-glucuronidase inhibitor of C. oleophila WP5-19-1 was partially purified by trypsin hydrolysis, ultrafiltration (3 kDa), and Sephadex G-50 filtration. The partially purified β-glucuronidase inhibitor was stable from 30℃ to 60℃ and at pH 6.0 9.0, and showed residual inhibitory activity of about 80%.http://www.kjmycology.or.kr/article/?num=N0320500102β-glucuronidase inhibitorcandida oleophilawp5-19-1non-pathogenic wild yeastpartial purificationprobiotics |
| spellingShingle | Seon-Jeong Park Jong-Soo Lee Production and Characterization of the Beneficial β-glucuronidase Inhibitor from non pathogenic wild yeast, Candida oleophila WP5-19-1, and its effects on gut microbes 한국균학회지 β-glucuronidase inhibitor candida oleophila wp5-19-1 non-pathogenic wild yeast partial purification probiotics |
| title | Production and Characterization of the Beneficial β-glucuronidase Inhibitor from non pathogenic wild yeast, Candida oleophila WP5-19-1, and its effects on gut microbes |
| title_full | Production and Characterization of the Beneficial β-glucuronidase Inhibitor from non pathogenic wild yeast, Candida oleophila WP5-19-1, and its effects on gut microbes |
| title_fullStr | Production and Characterization of the Beneficial β-glucuronidase Inhibitor from non pathogenic wild yeast, Candida oleophila WP5-19-1, and its effects on gut microbes |
| title_full_unstemmed | Production and Characterization of the Beneficial β-glucuronidase Inhibitor from non pathogenic wild yeast, Candida oleophila WP5-19-1, and its effects on gut microbes |
| title_short | Production and Characterization of the Beneficial β-glucuronidase Inhibitor from non pathogenic wild yeast, Candida oleophila WP5-19-1, and its effects on gut microbes |
| title_sort | production and characterization of the beneficial β glucuronidase inhibitor from non pathogenic wild yeast candida oleophila wp5 19 1 and its effects on gut microbes |
| topic | β-glucuronidase inhibitor candida oleophila wp5-19-1 non-pathogenic wild yeast partial purification probiotics |
| url | http://www.kjmycology.or.kr/article/?num=N0320500102 |
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