Animal study on transplantation of human umbilical vein endothelial cells for corneal endothelial decompensation
AIM: To explore the feasibility of culturing human umbilical vein endothelial cells(HUVEC)on acellular corneal stroma and performing the posterior lamellar endothelial keratoplasty(PLEK)treating corneal endothelial decompensation.<p>METHODS: Thirty New-Zealand rabbits were divided into three g...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2014-06-01
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| Series: | Guoji Yanke Zazhi |
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| Online Access: | http://www.ies.net.cn/cn_publish/2014/6/201406007.pdf |
| _version_ | 1818162056549892096 |
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| author | Li Cui Xiang Ma Yan-Hui Zhao |
| author_facet | Li Cui Xiang Ma Yan-Hui Zhao |
| author_sort | Li Cui |
| collection | DOAJ |
| description | AIM: To explore the feasibility of culturing human umbilical vein endothelial cells(HUVEC)on acellular corneal stroma and performing the posterior lamellar endothelial keratoplasty(PLEK)treating corneal endothelial decompensation.<p>METHODS: Thirty New-Zealand rabbits were divided into three groups randomly, 10 rabbits for experimental group, 10 for stroma group and 10 for control group. Corneal endothelial cells were removed to establish animal model of corneal endothelial failure. PLEK was performed on the rabbits of experimental group and stroma group, and nothing was transplantated onto the rabbits of control group with the deep layer excised only. Postoperative observation was taken for 3mo. The degree of corneal edema and central corneal thickness were recorded for statistical analysis.<p>RESULTS: Corneas in experimental group were relieved in edema obviously compared with that in stroma group and the control group, and showed increased transparency 7d after the operation. The average density of endothelial cells was 2 026.4±129.3cells/mm<sup>2</sup>, and average central corneal thickness was 505.2±25.4μm in experimental group, while 1 535.6±114.5μm in stroma group and 1 493.5±70.2μm in control group 3mo after operation.<p>CONCLUSION:We achieved preliminary success in our study that culturing HUVEC on acellular corneal stroma and performing PLEK for corneal endothelial decompensation. HUVEC transplanted could survive <i>in vivo</i>, and have normal biological function of keeping cornea transparent. This study provides a new idea and a new way clinically for the treatment of corneal endothelial diseases. |
| first_indexed | 2024-12-11T16:27:36Z |
| format | Article |
| id | doaj.art-1a366d0dacc044c492471b35d985c8ff |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-11T16:27:36Z |
| publishDate | 2014-06-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-1a366d0dacc044c492471b35d985c8ff2022-12-22T00:58:41ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232014-06-011461009101210.3980/j.issn.1672-5123.2014.06.07Animal study on transplantation of human umbilical vein endothelial cells for corneal endothelial decompensationLi Cui0Xiang Ma1Yan-Hui Zhao2Department of Ophthalmology, the Third People's Hospital of Dalian,Dalian 116033,Liaoning Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, ChinaDepartment of Ophthalmology, Dalian Armed Police Hospital, Dalian 116013, Liaoning Province, ChinaAIM: To explore the feasibility of culturing human umbilical vein endothelial cells(HUVEC)on acellular corneal stroma and performing the posterior lamellar endothelial keratoplasty(PLEK)treating corneal endothelial decompensation.<p>METHODS: Thirty New-Zealand rabbits were divided into three groups randomly, 10 rabbits for experimental group, 10 for stroma group and 10 for control group. Corneal endothelial cells were removed to establish animal model of corneal endothelial failure. PLEK was performed on the rabbits of experimental group and stroma group, and nothing was transplantated onto the rabbits of control group with the deep layer excised only. Postoperative observation was taken for 3mo. The degree of corneal edema and central corneal thickness were recorded for statistical analysis.<p>RESULTS: Corneas in experimental group were relieved in edema obviously compared with that in stroma group and the control group, and showed increased transparency 7d after the operation. The average density of endothelial cells was 2 026.4±129.3cells/mm<sup>2</sup>, and average central corneal thickness was 505.2±25.4μm in experimental group, while 1 535.6±114.5μm in stroma group and 1 493.5±70.2μm in control group 3mo after operation.<p>CONCLUSION:We achieved preliminary success in our study that culturing HUVEC on acellular corneal stroma and performing PLEK for corneal endothelial decompensation. HUVEC transplanted could survive <i>in vivo</i>, and have normal biological function of keeping cornea transparent. This study provides a new idea and a new way clinically for the treatment of corneal endothelial diseases.http://www.ies.net.cn/cn_publish/2014/6/201406007.pdfhuman umbilical vein endothelial cellacellular corneal stromaposterior lamellar endothelial keratoplastyposterior lamellar endothelial keratoplasty |
| spellingShingle | Li Cui Xiang Ma Yan-Hui Zhao Animal study on transplantation of human umbilical vein endothelial cells for corneal endothelial decompensation Guoji Yanke Zazhi human umbilical vein endothelial cell acellular corneal stroma posterior lamellar endothelial keratoplasty posterior lamellar endothelial keratoplasty |
| title | Animal study on transplantation of human umbilical vein endothelial cells for corneal endothelial decompensation |
| title_full | Animal study on transplantation of human umbilical vein endothelial cells for corneal endothelial decompensation |
| title_fullStr | Animal study on transplantation of human umbilical vein endothelial cells for corneal endothelial decompensation |
| title_full_unstemmed | Animal study on transplantation of human umbilical vein endothelial cells for corneal endothelial decompensation |
| title_short | Animal study on transplantation of human umbilical vein endothelial cells for corneal endothelial decompensation |
| title_sort | animal study on transplantation of human umbilical vein endothelial cells for corneal endothelial decompensation |
| topic | human umbilical vein endothelial cell acellular corneal stroma posterior lamellar endothelial keratoplasty posterior lamellar endothelial keratoplasty |
| url | http://www.ies.net.cn/cn_publish/2014/6/201406007.pdf |
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