Optimization of PVA-Arabic Gum–Honey-based Electrospun Nanofibers as Candidate Carrier for Peptide and Protein Delivery
Nanofibers dressing exhibit several advantageous characteristics for accelerating wound healing, such as its similar structure to the extracellular matrix (ECM), high surface area/volume ratio, high porosity and high loading capacity of drug. The nanofibers dressing which were prepared by the el...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2019-08-01
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| Series: | Pharmaceutical Sciences and Research |
| Subjects: | |
| Online Access: | http://psr.ui.ac.id/index.php/journal/article/view/4552 |
| _version_ | 1818871436182290432 |
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| author | Ningsih Rezeki Khairurrijal Silvia Surini |
| author_facet | Ningsih Rezeki Khairurrijal Silvia Surini |
| author_sort | Ningsih Rezeki |
| collection | DOAJ |
| description | Nanofibers dressing exhibit several advantageous characteristics for accelerating wound healing, such
as its similar structure to the extracellular matrix (ECM), high surface area/volume ratio, high porosity
and high loading capacity of drug. The nanofibers dressing which were prepared by the electrospinning
technique using combination of synthetic and natural polymer excipients capable of fulfilling the ideal
wound dressing criteria. This study aimed to develop nanofibers dressing prepared from polyvinyl
alcohol (PVA), Arabic gum (GA) and honey by electrospinning method. This study focused on the effect
of electrospinning parameters and the morphology of electrospun nanofibers of the blended solutions
made from PVA (9% w/v) – GA (1% w/v) and honey (at varied concentrations of 0;1;3% w/v) with or
without Triton X-100 (0.05% w/v). The effect of varied process parameters such as voltage and flow rate
in electrospinning was also investigated. The blended solutions with the various concentration of honey
at 0;1;3% w/v and Triton X-100 were named as FAt, FBt, and FCt, respectively, while the blended
solutions without Triton X-100, were named as FA, FB, and FC. The optimum electrospining parameter
were 18 KV and 5 µl/minute for FAt, FBt, and FCt; and 20 KV and 10 µl/minute for FA, FB, and FC.
Electrospun nanofibers of FAt, FBt, FCt showed smoother and more uniform fibers in comparison to the
nanofibers FA, FB, and FC. The average nanofibers diameter of FAt, FBt, FCt were 244±45; 266±45;
283±57 nm, respectively, while the average nanofibers diameter of FA, FB, FC was 406±140, 457±168,
594±204 nm, respectively. Higher concentration of honey increased the diameters of nanofibers. The
average nanofibers diameter of FAt, FBt, and FCt were within nanoscale in range of the ECM (50-500
nm), which were suitable for accelerating wound healing. Therefore, this study indicated that PVA-GAhoney
nanofibers dressing is suitable to be further developed as carrier for growth factors. |
| first_indexed | 2024-12-19T12:22:53Z |
| format | Article |
| id | doaj.art-1a3765cdf4c04e9982a3814f6546dd7d |
| institution | Directory Open Access Journal |
| issn | 2407-2354 2477-0612 |
| language | English |
| last_indexed | 2024-12-19T12:22:53Z |
| publishDate | 2019-08-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Pharmaceutical Sciences and Research |
| spelling | doaj.art-1a3765cdf4c04e9982a3814f6546dd7d2022-12-21T20:21:42ZengUniversitas IndonesiaPharmaceutical Sciences and Research2407-23542477-06122019-08-01628998https://doi.org/10.7454/psr.v6i2.4552Optimization of PVA-Arabic Gum–Honey-based Electrospun Nanofibers as Candidate Carrier for Peptide and Protein DeliveryNingsih Rezeki0Khairurrijal1Silvia Surini2https://orcid.org/0000-0003-1211-9706Faculty of Pharmacy, Universitas Indonesia, Depok, IndonesiaDepartment of Physics, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung,Faculty of Pharmacy, Universitas Indonesia, Depok, IndonesiaNanofibers dressing exhibit several advantageous characteristics for accelerating wound healing, such as its similar structure to the extracellular matrix (ECM), high surface area/volume ratio, high porosity and high loading capacity of drug. The nanofibers dressing which were prepared by the electrospinning technique using combination of synthetic and natural polymer excipients capable of fulfilling the ideal wound dressing criteria. This study aimed to develop nanofibers dressing prepared from polyvinyl alcohol (PVA), Arabic gum (GA) and honey by electrospinning method. This study focused on the effect of electrospinning parameters and the morphology of electrospun nanofibers of the blended solutions made from PVA (9% w/v) – GA (1% w/v) and honey (at varied concentrations of 0;1;3% w/v) with or without Triton X-100 (0.05% w/v). The effect of varied process parameters such as voltage and flow rate in electrospinning was also investigated. The blended solutions with the various concentration of honey at 0;1;3% w/v and Triton X-100 were named as FAt, FBt, and FCt, respectively, while the blended solutions without Triton X-100, were named as FA, FB, and FC. The optimum electrospining parameter were 18 KV and 5 µl/minute for FAt, FBt, and FCt; and 20 KV and 10 µl/minute for FA, FB, and FC. Electrospun nanofibers of FAt, FBt, FCt showed smoother and more uniform fibers in comparison to the nanofibers FA, FB, and FC. The average nanofibers diameter of FAt, FBt, FCt were 244±45; 266±45; 283±57 nm, respectively, while the average nanofibers diameter of FA, FB, FC was 406±140, 457±168, 594±204 nm, respectively. Higher concentration of honey increased the diameters of nanofibers. The average nanofibers diameter of FAt, FBt, and FCt were within nanoscale in range of the ECM (50-500 nm), which were suitable for accelerating wound healing. Therefore, this study indicated that PVA-GAhoney nanofibers dressing is suitable to be further developed as carrier for growth factors.http://psr.ui.ac.id/index.php/journal/article/view/4552arabic gumelectrospinninghoneynanofiberspolyvinyl alcohol |
| spellingShingle | Ningsih Rezeki Khairurrijal Silvia Surini Optimization of PVA-Arabic Gum–Honey-based Electrospun Nanofibers as Candidate Carrier for Peptide and Protein Delivery Pharmaceutical Sciences and Research arabic gum electrospinning honey nanofibers polyvinyl alcohol |
| title | Optimization of PVA-Arabic Gum–Honey-based Electrospun Nanofibers as Candidate Carrier for Peptide and Protein Delivery |
| title_full | Optimization of PVA-Arabic Gum–Honey-based Electrospun Nanofibers as Candidate Carrier for Peptide and Protein Delivery |
| title_fullStr | Optimization of PVA-Arabic Gum–Honey-based Electrospun Nanofibers as Candidate Carrier for Peptide and Protein Delivery |
| title_full_unstemmed | Optimization of PVA-Arabic Gum–Honey-based Electrospun Nanofibers as Candidate Carrier for Peptide and Protein Delivery |
| title_short | Optimization of PVA-Arabic Gum–Honey-based Electrospun Nanofibers as Candidate Carrier for Peptide and Protein Delivery |
| title_sort | optimization of pva arabic gum honey based electrospun nanofibers as candidate carrier for peptide and protein delivery |
| topic | arabic gum electrospinning honey nanofibers polyvinyl alcohol |
| url | http://psr.ui.ac.id/index.php/journal/article/view/4552 |
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