<em>In vitro</em> establishment of axillary buds of <em>Bambusa vulgaris</em> var Vittata

Micropropagation is a viable alternative for bamboo propagation due to the potentialities this type of plant has for building, handicrafts confection and soil protection. The technique is an efficient alternative to plants propagation with a high genetic value and quality. The current research was d...

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Main Authors: Yudith García-Ramírez, Marisol Freire-Seijo, Lillien Fajardo, Marisol Tejeda, Maritza Reyes
Format: Article
Language:English
Published: Universidad Central Marta Abreu de Las Villas 2007-07-01
Series:Biotecnología Vegetal
Online Access:https://revista.ibp.co.cu/index.php/BV/article/view/373
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author Yudith García-Ramírez
Marisol Freire-Seijo
Lillien Fajardo
Marisol Tejeda
Maritza Reyes
author_facet Yudith García-Ramírez
Marisol Freire-Seijo
Lillien Fajardo
Marisol Tejeda
Maritza Reyes
author_sort Yudith García-Ramírez
collection DOAJ
description Micropropagation is a viable alternative for bamboo propagation due to the potentialities this type of plant has for building, handicrafts confection and soil protection. The technique is an efficient alternative to plants propagation with a high genetic value and quality. The current research was developed focused on the in vitro establishing of axillary buds of Bambusa vulgaris var. Vittata. Three concentrations of sodium hypochlorite (1.0, 2.0 and 3.0%) and three different times (10, 15, 20 minutes) were used for disinfection of axillary buds. The highest rates of established buds were achieved using sodium hypochlorite to 2.0% during 20 minutes for disinfection. Between 86% and 100% buds sprouted, and a 93.7% of explants free of visible microbial contaminants were obtained from axillary buds introduced to in vitro conditions. Keywords: bamboo, disinfection, sodium hypochlorite
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spelling doaj.art-1a382858e1e841f29e12c620c85f4f6b2022-12-21T19:01:58ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472007-07-0173345<em>In vitro</em> establishment of axillary buds of <em>Bambusa vulgaris</em> var VittataYudith García-RamírezMarisol Freire-SeijoLillien FajardoMarisol TejedaMaritza ReyesMicropropagation is a viable alternative for bamboo propagation due to the potentialities this type of plant has for building, handicrafts confection and soil protection. The technique is an efficient alternative to plants propagation with a high genetic value and quality. The current research was developed focused on the in vitro establishing of axillary buds of Bambusa vulgaris var. Vittata. Three concentrations of sodium hypochlorite (1.0, 2.0 and 3.0%) and three different times (10, 15, 20 minutes) were used for disinfection of axillary buds. The highest rates of established buds were achieved using sodium hypochlorite to 2.0% during 20 minutes for disinfection. Between 86% and 100% buds sprouted, and a 93.7% of explants free of visible microbial contaminants were obtained from axillary buds introduced to in vitro conditions. Keywords: bamboo, disinfection, sodium hypochloritehttps://revista.ibp.co.cu/index.php/BV/article/view/373
spellingShingle Yudith García-Ramírez
Marisol Freire-Seijo
Lillien Fajardo
Marisol Tejeda
Maritza Reyes
<em>In vitro</em> establishment of axillary buds of <em>Bambusa vulgaris</em> var Vittata
Biotecnología Vegetal
title <em>In vitro</em> establishment of axillary buds of <em>Bambusa vulgaris</em> var Vittata
title_full <em>In vitro</em> establishment of axillary buds of <em>Bambusa vulgaris</em> var Vittata
title_fullStr <em>In vitro</em> establishment of axillary buds of <em>Bambusa vulgaris</em> var Vittata
title_full_unstemmed <em>In vitro</em> establishment of axillary buds of <em>Bambusa vulgaris</em> var Vittata
title_short <em>In vitro</em> establishment of axillary buds of <em>Bambusa vulgaris</em> var Vittata
title_sort em in vitro em establishment of axillary buds of em bambusa vulgaris em var vittata
url https://revista.ibp.co.cu/index.php/BV/article/view/373
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