Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+
Abstract Background This study aims to describe immunophenotypic explorations at diagnosis and follow up of a pediatric patient with leukemic phase of ALK+ anaplastic large cell lymphoma (ALCL) by multiparametric flow cytometry (MFC). Case An 8‐color MFC combination of antibodies allowed to identify...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | Cancer Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cnr2.1526 |
| _version_ | 1818042413840596992 |
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| author | Maria Clara Canellas Enrico Bruno‐Riscarolli Cristiane S. Ferreira‐Facio Daiana V. Lopes‐Alves Vitor D. Botafogo Deborah Sutter Roberia M. Pontes Marcelo G. P. Land Cristiane Bedran Milito Elaine Sobral daCosta |
| author_facet | Maria Clara Canellas Enrico Bruno‐Riscarolli Cristiane S. Ferreira‐Facio Daiana V. Lopes‐Alves Vitor D. Botafogo Deborah Sutter Roberia M. Pontes Marcelo G. P. Land Cristiane Bedran Milito Elaine Sobral daCosta |
| author_sort | Maria Clara Canellas |
| collection | DOAJ |
| description | Abstract Background This study aims to describe immunophenotypic explorations at diagnosis and follow up of a pediatric patient with leukemic phase of ALK+ anaplastic large cell lymphoma (ALCL) by multiparametric flow cytometry (MFC). Case An 8‐color MFC combination of antibodies allowed to identify neoplastic cells in concentrations until 0.02% during minimal residual disease (MRD) monitoring. Immunophenotypic shifts occurred in key markers as CD30, CD7, CD2, and CD5, however neoplastic cells were clearly discriminated from normal populations. Conclusion MFC can be a useful tool for ALCL diagnosis and MRD monitoring and may support therapeutic decisions. |
| first_indexed | 2024-12-10T08:45:56Z |
| format | Article |
| id | doaj.art-1a388375dccf4d52854162626535eb8b |
| institution | Directory Open Access Journal |
| issn | 2573-8348 |
| language | English |
| last_indexed | 2024-12-10T08:45:56Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Reports |
| spelling | doaj.art-1a388375dccf4d52854162626535eb8b2022-12-22T01:55:43ZengWileyCancer Reports2573-83482022-07-0157n/an/a10.1002/cnr2.1526Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+Maria Clara Canellas0Enrico Bruno‐Riscarolli1Cristiane S. Ferreira‐Facio2Daiana V. Lopes‐Alves3Vitor D. Botafogo4Deborah Sutter5Roberia M. Pontes6Marcelo G. P. Land7Cristiane Bedran Milito8Elaine Sobral daCosta9Martagão Gesteira Pediatric Institute – Federal University of Rio de Janeiro Rio de Janeiro BrazilMartagão Gesteira Pediatric Institute – Federal University of Rio de Janeiro Rio de Janeiro BrazilMartagão Gesteira Pediatric Institute – Federal University of Rio de Janeiro Rio de Janeiro BrazilMartagão Gesteira Pediatric Institute – Federal University of Rio de Janeiro Rio de Janeiro BrazilMartagão Gesteira Pediatric Institute – Federal University of Rio de Janeiro Rio de Janeiro BrazilMartagão Gesteira Pediatric Institute – Federal University of Rio de Janeiro Rio de Janeiro BrazilMartagão Gesteira Pediatric Institute – Federal University of Rio de Janeiro Rio de Janeiro BrazilMartagão Gesteira Pediatric Institute – Federal University of Rio de Janeiro Rio de Janeiro BrazilDepartment of Pathology, Faculty of Medicine Federal University of Rio de Janeiro Rio de Janeiro BrazilMartagão Gesteira Pediatric Institute – Federal University of Rio de Janeiro Rio de Janeiro BrazilAbstract Background This study aims to describe immunophenotypic explorations at diagnosis and follow up of a pediatric patient with leukemic phase of ALK+ anaplastic large cell lymphoma (ALCL) by multiparametric flow cytometry (MFC). Case An 8‐color MFC combination of antibodies allowed to identify neoplastic cells in concentrations until 0.02% during minimal residual disease (MRD) monitoring. Immunophenotypic shifts occurred in key markers as CD30, CD7, CD2, and CD5, however neoplastic cells were clearly discriminated from normal populations. Conclusion MFC can be a useful tool for ALCL diagnosis and MRD monitoring and may support therapeutic decisions.https://doi.org/10.1002/cnr2.1526anaplastic large cell lymphomaminimal residual diseasemultiparameter flow cytometry |
| spellingShingle | Maria Clara Canellas Enrico Bruno‐Riscarolli Cristiane S. Ferreira‐Facio Daiana V. Lopes‐Alves Vitor D. Botafogo Deborah Sutter Roberia M. Pontes Marcelo G. P. Land Cristiane Bedran Milito Elaine Sobral daCosta Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ Cancer Reports anaplastic large cell lymphoma minimal residual disease multiparameter flow cytometry |
| title | Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ |
| title_full | Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ |
| title_fullStr | Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ |
| title_full_unstemmed | Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ |
| title_short | Immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma ALK+ |
| title_sort | immunophenotypic shifts during minimal residual evaluation in a case of leukemic form of anaplastic large cell lymphoma alk |
| topic | anaplastic large cell lymphoma minimal residual disease multiparameter flow cytometry |
| url | https://doi.org/10.1002/cnr2.1526 |
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