Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair
Crosstalk between nerves and bone is essential for bone repair, for which Schwann cells (SCs) are crucial in the regulation of the microenvironment. Considering that exosomes are critical paracrine mediators for intercellular communication that exert important effects in tissue repair, the aim of th...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-09-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X23000518 |
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| author | Tianchang Wang Wentao Li Yuxin Zhang Xiang Xu Lei Qiang Weiqiang Miao Xiaokun Yue Xin Jiao Xianhao Zhou Zhenjiang Ma Shuai Li Muliang Ding Junfeng Zhu Chi Yang Hui Wang Tao Li Xin Sun Jinwu Wang |
| author_facet | Tianchang Wang Wentao Li Yuxin Zhang Xiang Xu Lei Qiang Weiqiang Miao Xiaokun Yue Xin Jiao Xianhao Zhou Zhenjiang Ma Shuai Li Muliang Ding Junfeng Zhu Chi Yang Hui Wang Tao Li Xin Sun Jinwu Wang |
| author_sort | Tianchang Wang |
| collection | DOAJ |
| description | Crosstalk between nerves and bone is essential for bone repair, for which Schwann cells (SCs) are crucial in the regulation of the microenvironment. Considering that exosomes are critical paracrine mediators for intercellular communication that exert important effects in tissue repair, the aim of this study is to confirm the function and molecular mechanisms of Schwann cell-derived exosomes (SC-exos) on bone regeneration and to propose engineered constructs that simulate SC-mediated nerve–bone crosstalk. SCs promoted the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) through exosomes. Subsequent molecular mechanism studies demonstrated that SC-exos promoted BMSC osteogenesis by regulating the TGF-β signaling pathway via let-7c-5p. Interestingly, SC-exos promoted the migration and tube formation performance of endothelial progenitor cells. Furthermore, the SC-exos@G/S constructs were developed by bioprinting technology that simulated SC-mediated nerve–bone crosstalk and improved the bone regeneration microenvironment by releasing SC-exos, exerting the regulatory effect of SCs in the microenvironment to promote innervation, vascularization, and osteogenesis and thus effectively improving bone repair in a cranial defect model. This study demonstrates the important role and underlying mechanism of SCs in regulating bone regeneration through SC-exos and provides a new engineered strategy for bone repair. |
| first_indexed | 2024-03-13T04:26:35Z |
| format | Article |
| id | doaj.art-1a3940e0fd8c4ada8301714ff6299231 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-03-13T04:26:35Z |
| publishDate | 2023-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-1a3940e0fd8c4ada8301714ff62992312023-06-20T04:20:25ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2023-09-0127377393Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repairTianchang Wang0Wentao Li1Yuxin Zhang2Xiang Xu3Lei Qiang4Weiqiang Miao5Xiaokun Yue6Xin Jiao7Xianhao Zhou8Zhenjiang Ma9Shuai Li10Muliang Ding11Junfeng Zhu12Chi Yang13Hui Wang14Tao Li15Xin Sun16Jinwu Wang17Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, ChinaSports Medicine Department, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, No.49, North Garden Road, Haidian District, Beijing, 100191, China; Peking University Institute of Sports Medicine, No.49, North Garden Road, Haidian District, Beijing, 100191, ChinaDepartment of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, ChinaShanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, ChinaSchool of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, ChinaShanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, ChinaShanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, ChinaShanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, ChinaShanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, ChinaShanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, ChinaDepartment of Orthopedics, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd, Hangzhou, 310003, ChinaDepartment of Orthopaedics, The Second Xiangya Hospital, Central South University, Changsha, 410001, Hunan, ChinaDepartment of Orthopedic Surgery, Xin Hua Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, No. 1665, Kongjiang Road, Shanghai, 200092, ChinaDepartment of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, ChinaInstitute of Rehabilitation Medicine, School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation, Ministry of Education, Shanghai, 201210, ChinaDepartment of Orthopedic Surgery, Xin Hua Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, No. 1665, Kongjiang Road, Shanghai, 200092, China; Corresponding author.Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, China; Corresponding author.Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, China; Institute of Rehabilitation Medicine, School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation, Ministry of Education, Shanghai, 201210, China; Corresponding author. Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, China.Crosstalk between nerves and bone is essential for bone repair, for which Schwann cells (SCs) are crucial in the regulation of the microenvironment. Considering that exosomes are critical paracrine mediators for intercellular communication that exert important effects in tissue repair, the aim of this study is to confirm the function and molecular mechanisms of Schwann cell-derived exosomes (SC-exos) on bone regeneration and to propose engineered constructs that simulate SC-mediated nerve–bone crosstalk. SCs promoted the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) through exosomes. Subsequent molecular mechanism studies demonstrated that SC-exos promoted BMSC osteogenesis by regulating the TGF-β signaling pathway via let-7c-5p. Interestingly, SC-exos promoted the migration and tube formation performance of endothelial progenitor cells. Furthermore, the SC-exos@G/S constructs were developed by bioprinting technology that simulated SC-mediated nerve–bone crosstalk and improved the bone regeneration microenvironment by releasing SC-exos, exerting the regulatory effect of SCs in the microenvironment to promote innervation, vascularization, and osteogenesis and thus effectively improving bone repair in a cranial defect model. This study demonstrates the important role and underlying mechanism of SCs in regulating bone regeneration through SC-exos and provides a new engineered strategy for bone repair.http://www.sciencedirect.com/science/article/pii/S2452199X23000518Schwann cellsMicroenvironmentNerve–bone crosstalkExosomesBioprinting |
| spellingShingle | Tianchang Wang Wentao Li Yuxin Zhang Xiang Xu Lei Qiang Weiqiang Miao Xiaokun Yue Xin Jiao Xianhao Zhou Zhenjiang Ma Shuai Li Muliang Ding Junfeng Zhu Chi Yang Hui Wang Tao Li Xin Sun Jinwu Wang Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair Bioactive Materials Schwann cells Microenvironment Nerve–bone crosstalk Exosomes Bioprinting |
| title | Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
| title_full | Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
| title_fullStr | Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
| title_full_unstemmed | Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
| title_short | Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
| title_sort | bioprinted constructs that simulate nerve bone crosstalk to improve microenvironment for bone repair |
| topic | Schwann cells Microenvironment Nerve–bone crosstalk Exosomes Bioprinting |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X23000518 |
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