RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls
<p>Abstract</p> <p>Background</p> <p>RNA from exosomes and other microvesicles contain transcripts of tumour origin. In this study we sought to identify biomarkers of glioblastoma multiforme in microvesicle RNA from serum of affected patients.</p> <p>Methods...
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BMC
2012-01-01
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| Series: | BMC Cancer |
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| Online Access: | http://www.biomedcentral.com/1471-2407/12/22 |
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| author | Noerholm Mikkel Balaj Leonora Limperg Tobias Salehi Afshin Zhu Lin Hochberg Fred H Breakefield Xandra O Carter Bob S Skog Johan |
| author_facet | Noerholm Mikkel Balaj Leonora Limperg Tobias Salehi Afshin Zhu Lin Hochberg Fred H Breakefield Xandra O Carter Bob S Skog Johan |
| author_sort | Noerholm Mikkel |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>RNA from exosomes and other microvesicles contain transcripts of tumour origin. In this study we sought to identify biomarkers of glioblastoma multiforme in microvesicle RNA from serum of affected patients.</p> <p>Methods</p> <p>Microvesicle RNA from serum from patients with de-novo primary glioblastoma multiforme (N = 9) and normal controls (N = 7) were analyzed by microarray analysis. Samples were collected according to protocols approved by the Institutional Review Board. Differential expressions were validated by qRT-PCR in a separate set of samples (N = 10 in both groups).</p> <p>Results</p> <p>Expression profiles of microvesicle RNA correctly separated individuals in two groups by unsupervised clustering. The most significant differences pertained to down-regulated genes (121 genes > 2-fold down) in the glioblastoma multiforme patient microvesicle RNA, validated by qRT-PCR on several genes. Overall, yields of microvesicle RNA from patients was higher than from normal controls, but the additional RNA was primarily of size < 500 nt. Gene ontology of the down-regulated genes indicated these are coding for ribosomal proteins and genes related to ribosome production.</p> <p>Conclusions</p> <p>Serum microvesicle RNA from patients with glioblastoma multiforme has significantly down-regulated levels of RNAs coding for ribosome production, compared to normal healthy controls, but a large overabundance of RNA of unknown origin with size < 500 nt.</p> |
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| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-20T10:55:03Z |
| publishDate | 2012-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-1a3f0aa560d34f37af9f50cbe420b5a22022-12-21T19:43:09ZengBMCBMC Cancer1471-24072012-01-011212210.1186/1471-2407-12-22RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controlsNoerholm MikkelBalaj LeonoraLimperg TobiasSalehi AfshinZhu LinHochberg Fred HBreakefield Xandra OCarter Bob SSkog Johan<p>Abstract</p> <p>Background</p> <p>RNA from exosomes and other microvesicles contain transcripts of tumour origin. In this study we sought to identify biomarkers of glioblastoma multiforme in microvesicle RNA from serum of affected patients.</p> <p>Methods</p> <p>Microvesicle RNA from serum from patients with de-novo primary glioblastoma multiforme (N = 9) and normal controls (N = 7) were analyzed by microarray analysis. Samples were collected according to protocols approved by the Institutional Review Board. Differential expressions were validated by qRT-PCR in a separate set of samples (N = 10 in both groups).</p> <p>Results</p> <p>Expression profiles of microvesicle RNA correctly separated individuals in two groups by unsupervised clustering. The most significant differences pertained to down-regulated genes (121 genes > 2-fold down) in the glioblastoma multiforme patient microvesicle RNA, validated by qRT-PCR on several genes. Overall, yields of microvesicle RNA from patients was higher than from normal controls, but the additional RNA was primarily of size < 500 nt. Gene ontology of the down-regulated genes indicated these are coding for ribosomal proteins and genes related to ribosome production.</p> <p>Conclusions</p> <p>Serum microvesicle RNA from patients with glioblastoma multiforme has significantly down-regulated levels of RNAs coding for ribosome production, compared to normal healthy controls, but a large overabundance of RNA of unknown origin with size < 500 nt.</p>http://www.biomedcentral.com/1471-2407/12/22ExosomesMicrovesiclesMicroarrayBiomarkersSerumGlioma |
| spellingShingle | Noerholm Mikkel Balaj Leonora Limperg Tobias Salehi Afshin Zhu Lin Hochberg Fred H Breakefield Xandra O Carter Bob S Skog Johan RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls BMC Cancer Exosomes Microvesicles Microarray Biomarkers Serum Glioma |
| title | RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls |
| title_full | RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls |
| title_fullStr | RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls |
| title_full_unstemmed | RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls |
| title_short | RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls |
| title_sort | rna expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls |
| topic | Exosomes Microvesicles Microarray Biomarkers Serum Glioma |
| url | http://www.biomedcentral.com/1471-2407/12/22 |
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