Single-nucleotide resolution detection of Topo IV cleavage activity in the Escherichia coli genome with Topo-Seq
Topoisomerase IV (Topo IV) is the main decatenation enzyme in Escherichia coli; it removes catenation links that are formed during DNA replication. Topo IV binding and cleavage sites were previously identified in the E. coli genome with ChIP-Seq and NorfIP. Here, we used a more sensitive, single-nuc...
Main Authors: | Dmitry Sutormin, Alina Galivondzhyan, Azamat Gafurov, Konstantin Severinov |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2023-04-01
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Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1160736/full |
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