Involvement of PRRSV NSP3 and NSP5 in the autophagy process
Abstract Background Autophagy is an essential process in eukaryotic cells in which autophagosomes form to deliver cellular organelles and long-lived proteins to lysosomes for degradation. Many studies have recently identified the regulatory mechanisms involved in the interaction between viral infect...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | Virology Journal |
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| Online Access: | http://link.springer.com/article/10.1186/s12985-019-1116-x |
| _version_ | 1818064258512977920 |
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| author | Wei Zhang Keren Chen Yang Guo Yaosheng Chen Xiaohong Liu |
| author_facet | Wei Zhang Keren Chen Yang Guo Yaosheng Chen Xiaohong Liu |
| author_sort | Wei Zhang |
| collection | DOAJ |
| description | Abstract Background Autophagy is an essential process in eukaryotic cells in which autophagosomes form to deliver cellular organelles and long-lived proteins to lysosomes for degradation. Many studies have recently identified the regulatory mechanisms involved in the interaction between viral infection and autophagy. Methods LC3 turnover and the proteins in the endoplasmic reticulum (ER) stress pathway were investigated using western blot analysis. The formation and degradation of autophagosomes were detected using immunofluorescence staining. Results Autophagy was activated by porcine reproductive and respiratory syndrome virus (PRRSV) NSP3, NSP5 and NSP9, which are two transmembrane proteins and an RNA-dependent RNA polymerase, respectively. The formation of autophagosomes was induced by NSP3 and NSP5 and developed from the ER; the fusion of these autophagosomes with lysosomes was limited. Although NSP3 and NSP5 are ER transmembrane proteins, these proteins did not activate the ER stress signaling pathways. In addition, the cytoplasmic domain of NSP3 plays a pivotal role in activating autophagy. Conclusions The data presented in this study reveal an important relationship between PRRSV NSPs and autophagy and provide new insights that improve our understanding of the involvement of PRRSV NSPs in the autophagy process. |
| first_indexed | 2024-12-10T14:33:08Z |
| format | Article |
| id | doaj.art-1a4441fd52b44ef29f77f18fd9044aa3 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-10T14:33:08Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-1a4441fd52b44ef29f77f18fd9044aa32022-12-22T01:44:53ZengBMCVirology Journal1743-422X2019-01-0116111110.1186/s12985-019-1116-xInvolvement of PRRSV NSP3 and NSP5 in the autophagy processWei Zhang0Keren Chen1Yang Guo2Yaosheng Chen3Xiaohong Liu4State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen UniversityState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen UniversityState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen UniversityState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen UniversityState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen UniversityAbstract Background Autophagy is an essential process in eukaryotic cells in which autophagosomes form to deliver cellular organelles and long-lived proteins to lysosomes for degradation. Many studies have recently identified the regulatory mechanisms involved in the interaction between viral infection and autophagy. Methods LC3 turnover and the proteins in the endoplasmic reticulum (ER) stress pathway were investigated using western blot analysis. The formation and degradation of autophagosomes were detected using immunofluorescence staining. Results Autophagy was activated by porcine reproductive and respiratory syndrome virus (PRRSV) NSP3, NSP5 and NSP9, which are two transmembrane proteins and an RNA-dependent RNA polymerase, respectively. The formation of autophagosomes was induced by NSP3 and NSP5 and developed from the ER; the fusion of these autophagosomes with lysosomes was limited. Although NSP3 and NSP5 are ER transmembrane proteins, these proteins did not activate the ER stress signaling pathways. In addition, the cytoplasmic domain of NSP3 plays a pivotal role in activating autophagy. Conclusions The data presented in this study reveal an important relationship between PRRSV NSPs and autophagy and provide new insights that improve our understanding of the involvement of PRRSV NSPs in the autophagy process.http://link.springer.com/article/10.1186/s12985-019-1116-xPorcine reproductive and respiratory syndrome virusAutophagosomesEndoplasmic reticulumNSP3 and NSP5Cytoplasmic domain |
| spellingShingle | Wei Zhang Keren Chen Yang Guo Yaosheng Chen Xiaohong Liu Involvement of PRRSV NSP3 and NSP5 in the autophagy process Virology Journal Porcine reproductive and respiratory syndrome virus Autophagosomes Endoplasmic reticulum NSP3 and NSP5 Cytoplasmic domain |
| title | Involvement of PRRSV NSP3 and NSP5 in the autophagy process |
| title_full | Involvement of PRRSV NSP3 and NSP5 in the autophagy process |
| title_fullStr | Involvement of PRRSV NSP3 and NSP5 in the autophagy process |
| title_full_unstemmed | Involvement of PRRSV NSP3 and NSP5 in the autophagy process |
| title_short | Involvement of PRRSV NSP3 and NSP5 in the autophagy process |
| title_sort | involvement of prrsv nsp3 and nsp5 in the autophagy process |
| topic | Porcine reproductive and respiratory syndrome virus Autophagosomes Endoplasmic reticulum NSP3 and NSP5 Cytoplasmic domain |
| url | http://link.springer.com/article/10.1186/s12985-019-1116-x |
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