Cafeteria Diet-Induced Obesity Worsens Experimental CKD
Obesity is a significant risk factor for chronic kidney disease (CKD). This study aimed to evaluate the impact of obesity on the development of kidney fibrosis in a model of cafeteria diet rats undergoing 5/6th nephrectomy (SNx). Collagen 1, 3, and 4 expression, adipocyte size, macrophage number, an...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/15/15/3331 |
| _version_ | 1797586211794059264 |
|---|---|
| author | Jonas Laget Irene Cortijo Juliana H. Boukhaled Karen Muyor Flore Duranton Bernard Jover Fabrice Raynaud Anne-Dominique Lajoix Àngel Argilés Nathalie Gayrard |
| author_facet | Jonas Laget Irene Cortijo Juliana H. Boukhaled Karen Muyor Flore Duranton Bernard Jover Fabrice Raynaud Anne-Dominique Lajoix Àngel Argilés Nathalie Gayrard |
| author_sort | Jonas Laget |
| collection | DOAJ |
| description | Obesity is a significant risk factor for chronic kidney disease (CKD). This study aimed to evaluate the impact of obesity on the development of kidney fibrosis in a model of cafeteria diet rats undergoing 5/6th nephrectomy (SNx). Collagen 1, 3, and 4 expression, adipocyte size, macrophage number, and the expression of 30 adipokines were determined. Collagen 1 expression in kidney tissue was increased in Standard-SNx and Cafeteria-SNx (7.1 ± 0.6% and 8.9 ± 0.9 tissue area, respectively). Renal expression of collagen 3 and 4 was significantly increased (<i>p</i> < 0.05) in Cafeteria-SNx (8.6 ± 1.5 and 10.9 ± 1.9% tissue area, respectively) compared to Cafeteria (5.2 ± 0.5 and 6.3 ± 0.6% tissue area, respectively). Adipocyte size in eWAT was significantly increased by the cafeteria diet. In Cafeteria-SNx, we observed a significant increase in macrophage number in the kidney (<i>p</i> = 0.01) and a consistent tendency in eWAT. The adipokine level was higher in the Cafeteria groups. Interleukin 11, dipeptidyl peptidase 4, and serpin 1 were increased in Cafeteria-SNx. In the kidney, collagen 3 and 4 expressions and the number of macrophages were increased in Cafeteria-SNx, suggesting an exacerbation by preexisting obesity of CKD-induced renal inflammation and fibrosis. IL11, DPP4, and serpin 1 can act directly on fibrosis and participate in the observed worsening CKD. |
| first_indexed | 2024-03-11T00:19:06Z |
| format | Article |
| id | doaj.art-1a445c347ec849d8a3b9f6c4ec2c9cbf |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T00:19:06Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-1a445c347ec849d8a3b9f6c4ec2c9cbf2023-11-18T23:23:06ZengMDPI AGNutrients2072-66432023-07-011515333110.3390/nu15153331Cafeteria Diet-Induced Obesity Worsens Experimental CKDJonas Laget0Irene Cortijo1Juliana H. Boukhaled2Karen Muyor3Flore Duranton4Bernard Jover5Fabrice Raynaud6Anne-Dominique Lajoix7Àngel Argilés8Nathalie Gayrard9RD-Néphrologie, 34090 Montpellier, FranceRD-Néphrologie, 34090 Montpellier, FranceRD-Néphrologie, 34090 Montpellier, FranceRD-Néphrologie, 34090 Montpellier, FranceRD-Néphrologie, 34090 Montpellier, FranceRD-Néphrologie, 34090 Montpellier, FrancePhyMedExp, INSERM, CNRS, Université de Montpellier, 34090 Montpellier, FranceBiocommunication in Cardio-Metabolism (BC2M), University of Montpellier, 34090 Montpellier, FranceRD-Néphrologie, 34090 Montpellier, FranceRD-Néphrologie, 34090 Montpellier, FranceObesity is a significant risk factor for chronic kidney disease (CKD). This study aimed to evaluate the impact of obesity on the development of kidney fibrosis in a model of cafeteria diet rats undergoing 5/6th nephrectomy (SNx). Collagen 1, 3, and 4 expression, adipocyte size, macrophage number, and the expression of 30 adipokines were determined. Collagen 1 expression in kidney tissue was increased in Standard-SNx and Cafeteria-SNx (7.1 ± 0.6% and 8.9 ± 0.9 tissue area, respectively). Renal expression of collagen 3 and 4 was significantly increased (<i>p</i> < 0.05) in Cafeteria-SNx (8.6 ± 1.5 and 10.9 ± 1.9% tissue area, respectively) compared to Cafeteria (5.2 ± 0.5 and 6.3 ± 0.6% tissue area, respectively). Adipocyte size in eWAT was significantly increased by the cafeteria diet. In Cafeteria-SNx, we observed a significant increase in macrophage number in the kidney (<i>p</i> = 0.01) and a consistent tendency in eWAT. The adipokine level was higher in the Cafeteria groups. Interleukin 11, dipeptidyl peptidase 4, and serpin 1 were increased in Cafeteria-SNx. In the kidney, collagen 3 and 4 expressions and the number of macrophages were increased in Cafeteria-SNx, suggesting an exacerbation by preexisting obesity of CKD-induced renal inflammation and fibrosis. IL11, DPP4, and serpin 1 can act directly on fibrosis and participate in the observed worsening CKD.https://www.mdpi.com/2072-6643/15/15/3331chronic kidney diseaseobesityadipokinefibrosiscollagenmacrophage |
| spellingShingle | Jonas Laget Irene Cortijo Juliana H. Boukhaled Karen Muyor Flore Duranton Bernard Jover Fabrice Raynaud Anne-Dominique Lajoix Àngel Argilés Nathalie Gayrard Cafeteria Diet-Induced Obesity Worsens Experimental CKD Nutrients chronic kidney disease obesity adipokine fibrosis collagen macrophage |
| title | Cafeteria Diet-Induced Obesity Worsens Experimental CKD |
| title_full | Cafeteria Diet-Induced Obesity Worsens Experimental CKD |
| title_fullStr | Cafeteria Diet-Induced Obesity Worsens Experimental CKD |
| title_full_unstemmed | Cafeteria Diet-Induced Obesity Worsens Experimental CKD |
| title_short | Cafeteria Diet-Induced Obesity Worsens Experimental CKD |
| title_sort | cafeteria diet induced obesity worsens experimental ckd |
| topic | chronic kidney disease obesity adipokine fibrosis collagen macrophage |
| url | https://www.mdpi.com/2072-6643/15/15/3331 |
| work_keys_str_mv | AT jonaslaget cafeteriadietinducedobesityworsensexperimentalckd AT irenecortijo cafeteriadietinducedobesityworsensexperimentalckd AT julianahboukhaled cafeteriadietinducedobesityworsensexperimentalckd AT karenmuyor cafeteriadietinducedobesityworsensexperimentalckd AT floreduranton cafeteriadietinducedobesityworsensexperimentalckd AT bernardjover cafeteriadietinducedobesityworsensexperimentalckd AT fabriceraynaud cafeteriadietinducedobesityworsensexperimentalckd AT annedominiquelajoix cafeteriadietinducedobesityworsensexperimentalckd AT angelargiles cafeteriadietinducedobesityworsensexperimentalckd AT nathaliegayrard cafeteriadietinducedobesityworsensexperimentalckd |