m6A and miRNA jointly regulate the development of breast muscles in duck embryonic stages
N6-methyladenosine (m6A) is an abundant internal mRNA modification and plays a crucial regulatory role in animal growth and development. In recent years, m6A modification has been found to play a key role in skeletal muscles. However, whether m6A modification contributes to embryonic breast muscle d...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.933850/full |
| _version_ | 1817978150875824128 |
|---|---|
| author | Lihong Gu Lihong Gu Shunjin Zhang Boling Li Qicheng Jiang Tieshan Xu Yongzhen Huang Dajie Lin Manping Xing Manping Xing Lili Huang Lili Huang Xinli Zheng Xinli Zheng Feng Wang Zhe Chao Zhe Chao Weiping Sun |
| author_facet | Lihong Gu Lihong Gu Shunjin Zhang Boling Li Qicheng Jiang Tieshan Xu Yongzhen Huang Dajie Lin Manping Xing Manping Xing Lili Huang Lili Huang Xinli Zheng Xinli Zheng Feng Wang Zhe Chao Zhe Chao Weiping Sun |
| author_sort | Lihong Gu |
| collection | DOAJ |
| description | N6-methyladenosine (m6A) is an abundant internal mRNA modification and plays a crucial regulatory role in animal growth and development. In recent years, m6A modification has been found to play a key role in skeletal muscles. However, whether m6A modification contributes to embryonic breast muscle development of Pekin ducks has not been explored. To explore the role of m6A in embryonic breast muscle development of ducks, we performed m6A sequencing and miRNA sequencing for the breast muscle of duck embryos on the 19th (E19) and 27th (E27) days. A total of 12,717 m6A peaks were identified at E19, representing a total of 7,438 gene transcripts. A total of 14,703 m6A peaks were identified, which overlapped with the transcripts of 7,753 genes at E27. Comparing E19 and E27, we identified 2,347 differential m6A peaks, which overlapped with 1,605 m6A-modified genes (MMGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that MMGs were enriched in multiple muscle- or fat-related pathways, which was also revealed from our analysis of differentially expressed genes (DEGs). Conjoint analysis of m6A-seq and RNA-seq data showed that pathways related to β-oxidation of fatty acids and skeletal muscle development were significantly enriched, suggesting that m6A modification is involved in the regulation of fat deposition and skeletal muscle development. There were 90 upregulated and 102 downregulated miRNAs identified between the E19 and E27 stages. Through overlapping analysis of genes shared by MMGs and DEGs and the targets of differentially expressed miRNAs (DEMs), we identified six m6A-mRNA-regulated miRNAs. Finally, we found that m6A modification can regulate fat deposition and skeletal muscle development. In conclusion, our results suggest that m6A modification is a key regulator for embryonic breast muscle development and fat deposition of ducks by affecting expressions of mRNAs and miRNAs. This is the first study to comprehensively characterize the m6A patterns in the duck transcriptome. These data provide a solid basis for future work aimed at determining the potential functional roles of m6A modification in adipose deposition and muscle growth. |
| first_indexed | 2024-04-13T22:25:35Z |
| format | Article |
| id | doaj.art-1a49b3bb710d4ff4876f1d28201295df |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-13T22:25:35Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-1a49b3bb710d4ff4876f1d28201295df2022-12-22T02:27:04ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-10-01910.3389/fvets.2022.933850933850m6A and miRNA jointly regulate the development of breast muscles in duck embryonic stagesLihong Gu0Lihong Gu1Shunjin Zhang2Boling Li3Qicheng Jiang4Tieshan Xu5Yongzhen Huang6Dajie Lin7Manping Xing8Manping Xing9Lili Huang10Lili Huang11Xinli Zheng12Xinli Zheng13Feng Wang14Zhe Chao15Zhe Chao16Weiping Sun17Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaInstitute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang, ChinaThe Hainan Animal Husbandry Technology Promotion Station, Haikou, ChinaSchool of Life Science, Hainan University, Haikou, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang, ChinaInstitute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, ChinaInstitute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, ChinaKey Laboratory of Tropical Animal Breeding and Disease Research, Haikou, ChinaInstitute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, ChinaKey Laboratory of Tropical Animal Breeding and Disease Research, Haikou, ChinaInstitute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, ChinaKey Laboratory of Tropical Animal Breeding and Disease Research, Haikou, ChinaInstitute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, ChinaInstitute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, ChinaKey Laboratory of Tropical Animal Breeding and Disease Research, Haikou, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaN6-methyladenosine (m6A) is an abundant internal mRNA modification and plays a crucial regulatory role in animal growth and development. In recent years, m6A modification has been found to play a key role in skeletal muscles. However, whether m6A modification contributes to embryonic breast muscle development of Pekin ducks has not been explored. To explore the role of m6A in embryonic breast muscle development of ducks, we performed m6A sequencing and miRNA sequencing for the breast muscle of duck embryos on the 19th (E19) and 27th (E27) days. A total of 12,717 m6A peaks were identified at E19, representing a total of 7,438 gene transcripts. A total of 14,703 m6A peaks were identified, which overlapped with the transcripts of 7,753 genes at E27. Comparing E19 and E27, we identified 2,347 differential m6A peaks, which overlapped with 1,605 m6A-modified genes (MMGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that MMGs were enriched in multiple muscle- or fat-related pathways, which was also revealed from our analysis of differentially expressed genes (DEGs). Conjoint analysis of m6A-seq and RNA-seq data showed that pathways related to β-oxidation of fatty acids and skeletal muscle development were significantly enriched, suggesting that m6A modification is involved in the regulation of fat deposition and skeletal muscle development. There were 90 upregulated and 102 downregulated miRNAs identified between the E19 and E27 stages. Through overlapping analysis of genes shared by MMGs and DEGs and the targets of differentially expressed miRNAs (DEMs), we identified six m6A-mRNA-regulated miRNAs. Finally, we found that m6A modification can regulate fat deposition and skeletal muscle development. In conclusion, our results suggest that m6A modification is a key regulator for embryonic breast muscle development and fat deposition of ducks by affecting expressions of mRNAs and miRNAs. This is the first study to comprehensively characterize the m6A patterns in the duck transcriptome. These data provide a solid basis for future work aimed at determining the potential functional roles of m6A modification in adipose deposition and muscle growth.https://www.frontiersin.org/articles/10.3389/fvets.2022.933850/fullducksembryobreast musclesm6A sequencingmiRNAs sequencing |
| spellingShingle | Lihong Gu Lihong Gu Shunjin Zhang Boling Li Qicheng Jiang Tieshan Xu Yongzhen Huang Dajie Lin Manping Xing Manping Xing Lili Huang Lili Huang Xinli Zheng Xinli Zheng Feng Wang Zhe Chao Zhe Chao Weiping Sun m6A and miRNA jointly regulate the development of breast muscles in duck embryonic stages Frontiers in Veterinary Science ducks embryo breast muscles m6A sequencing miRNAs sequencing |
| title | m6A and miRNA jointly regulate the development of breast muscles in duck embryonic stages |
| title_full | m6A and miRNA jointly regulate the development of breast muscles in duck embryonic stages |
| title_fullStr | m6A and miRNA jointly regulate the development of breast muscles in duck embryonic stages |
| title_full_unstemmed | m6A and miRNA jointly regulate the development of breast muscles in duck embryonic stages |
| title_short | m6A and miRNA jointly regulate the development of breast muscles in duck embryonic stages |
| title_sort | m6a and mirna jointly regulate the development of breast muscles in duck embryonic stages |
| topic | ducks embryo breast muscles m6A sequencing miRNAs sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.933850/full |
| work_keys_str_mv | AT lihonggu m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT lihonggu m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT shunjinzhang m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT bolingli m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT qichengjiang m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT tieshanxu m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT yongzhenhuang m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT dajielin m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT manpingxing m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT manpingxing m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT lilihuang m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT lilihuang m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT xinlizheng m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT xinlizheng m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT fengwang m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT zhechao m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT zhechao m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages AT weipingsun m6aandmirnajointlyregulatethedevelopmentofbreastmusclesinduckembryonicstages |