Application of standard and molecular methods for the diagnosis of newcastle disease
Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from fo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2012-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641204433V.pdf |
| _version_ | 1818932617453502464 |
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| author | Vidanović D. Šekler M. Polaček V. Vasković N. Ašanin Ružica Milić N. Nišavić J. |
| author_facet | Vidanović D. Šekler M. Polaček V. Vasković N. Ašanin Ružica Milić N. Nišavić J. |
| author_sort | Vidanović D. |
| collection | DOAJ |
| description | Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId. [Projekat Ministarstva nauke Republike Srbije, br. 31008] |
| first_indexed | 2024-12-20T04:35:20Z |
| format | Article |
| id | doaj.art-1a49b63f39854f27878ce8e367c3d240 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-20T04:35:20Z |
| publishDate | 2012-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-1a49b63f39854f27878ce8e367c3d2402022-12-21T19:53:16ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392012-01-016441433143710.2298/ABS1204433VApplication of standard and molecular methods for the diagnosis of newcastle diseaseVidanović D.Šekler M.Polaček V.Vasković N.Ašanin RužicaMilić N.Nišavić J.Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId. [Projekat Ministarstva nauke Republike Srbije, br. 31008]http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641204433V.pdfNewcastle disease virusRT-PCRReal Time RT-PCR |
| spellingShingle | Vidanović D. Šekler M. Polaček V. Vasković N. Ašanin Ružica Milić N. Nišavić J. Application of standard and molecular methods for the diagnosis of newcastle disease Archives of Biological Sciences Newcastle disease virus RT-PCR Real Time RT-PCR |
| title | Application of standard and molecular methods for the diagnosis of newcastle disease |
| title_full | Application of standard and molecular methods for the diagnosis of newcastle disease |
| title_fullStr | Application of standard and molecular methods for the diagnosis of newcastle disease |
| title_full_unstemmed | Application of standard and molecular methods for the diagnosis of newcastle disease |
| title_short | Application of standard and molecular methods for the diagnosis of newcastle disease |
| title_sort | application of standard and molecular methods for the diagnosis of newcastle disease |
| topic | Newcastle disease virus RT-PCR Real Time RT-PCR |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641204433V.pdf |
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