Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats
Objectives: Induced dilated cardiomyopathy is the main limitation of the anti-cancer drug doxorubicin, which causes oxidative stress and cardiomyocyte death. As ozone therapy can activate the antioxidant systems, this study aimed to investigate the therapeutic efficacy of ozone-oxidative preconditio...
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| Format: | Article |
| Language: | English |
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Sultan Qaboos University
2014-08-01
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| Series: | Sultan Qaboos University Medical Journal |
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| Online Access: | http://web.squ.edu.om/squmj/includes/tng/pub/tNG_download.asp?id=fc7e8652c33e23df03e61b5298e4ff56 |
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| author | Livan Delgado-Roche Yanet Hernández-Matos Emilio A. Medina Dalia Á. Morejón Maité R. González Gregorio Martίnez-Sánchez |
| author_facet | Livan Delgado-Roche Yanet Hernández-Matos Emilio A. Medina Dalia Á. Morejón Maité R. González Gregorio Martίnez-Sánchez |
| author_sort | Livan Delgado-Roche |
| collection | DOAJ |
| description | Objectives: Induced dilated cardiomyopathy is the main limitation of the anti-cancer drug doxorubicin, which causes oxidative stress and cardiomyocyte death. As ozone therapy can activate the antioxidant systems, this study aimed to investigate the therapeutic efficacy of ozone-oxidative preconditioning against doxorubicin-induced cardiotoxicity. Methods: The study was carried out from September 2013 to January 2014. Sprague-Dawley rats were randomly distributed in the following treatment groups: Group 1 were treated with 2 mg/kg intraperitoneal (i.p.) of doxorubicin twice a week for 50 days; Group 2 were treated with 0.3 mg of ozone/oxygen mixture at 50 μg/ mL of ozone per 6 mL of oxygen by rectal insufflation and then treated with doxorubicin; Group 3 were treated as Group 2 but only with the oxygen, and Group 4 were treated with oxygen first, and then with sodium chloride i.p. as the control group. Results: The results showed that ozone therapy preserved left ventricle morphology which was
accompanied by a reduction of serum pro-brain natriuretic peptide levels. The cardioprotective effects of ozoneoxidative preconditioning were associated with a significant increase (P <0.05) of antioxidant enzymes activities and a reduction of lipid and protein oxidation (P <0.05). Conclusion: Ozone-oxidative preconditioning prevents doxorubicin-induced dilated cardiomyopathy through an increase of antioxidant enzymes and a reduction of oxidised macromolecules. This establishes the background for future studies to determine if ozone therapy can be used as a complementary treatment for attenuating doxorubicin-induced cardiotoxicity in cancer patients. |
| first_indexed | 2024-04-12T18:43:07Z |
| format | Article |
| id | doaj.art-1a4c4baab6bf40edb4f28a46ac8e7cc1 |
| institution | Directory Open Access Journal |
| issn | 2075-051X 2075-0528 |
| language | English |
| last_indexed | 2024-04-12T18:43:07Z |
| publishDate | 2014-08-01 |
| publisher | Sultan Qaboos University |
| record_format | Article |
| series | Sultan Qaboos University Medical Journal |
| spelling | doaj.art-1a4c4baab6bf40edb4f28a46ac8e7cc12022-12-22T03:20:42ZengSultan Qaboos UniversitySultan Qaboos University Medical Journal2075-051X2075-05282014-08-01143e342e348Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley RatsLivan Delgado-Roche0Yanet Hernández-Matos1Emilio A. Medina2Dalia Á. Morejón3Maité R. González4Gregorio Martίnez-Sánchez5Center of Studies for Research & Biological Evaluations, Pharmacy & Food Science College, University of Havana, CubaCenter of Studies for Research & Biological Evaluations, Pharmacy & Food Science College, University of Havana, CubaCenter of Studies for Research & Biological Evaluations, Pharmacy & Food Science College, University of Havana, CubaCenter of Studies for Research & Biological Evaluations, Pharmacy & Food Science College, University of Havana, CubaLABIOFAM Pharmaceutical Laboratories, Havana, CubaMedical Center Beauty Benefit, Osimo, Ancona, ItalyObjectives: Induced dilated cardiomyopathy is the main limitation of the anti-cancer drug doxorubicin, which causes oxidative stress and cardiomyocyte death. As ozone therapy can activate the antioxidant systems, this study aimed to investigate the therapeutic efficacy of ozone-oxidative preconditioning against doxorubicin-induced cardiotoxicity. Methods: The study was carried out from September 2013 to January 2014. Sprague-Dawley rats were randomly distributed in the following treatment groups: Group 1 were treated with 2 mg/kg intraperitoneal (i.p.) of doxorubicin twice a week for 50 days; Group 2 were treated with 0.3 mg of ozone/oxygen mixture at 50 μg/ mL of ozone per 6 mL of oxygen by rectal insufflation and then treated with doxorubicin; Group 3 were treated as Group 2 but only with the oxygen, and Group 4 were treated with oxygen first, and then with sodium chloride i.p. as the control group. Results: The results showed that ozone therapy preserved left ventricle morphology which was accompanied by a reduction of serum pro-brain natriuretic peptide levels. The cardioprotective effects of ozoneoxidative preconditioning were associated with a significant increase (P <0.05) of antioxidant enzymes activities and a reduction of lipid and protein oxidation (P <0.05). Conclusion: Ozone-oxidative preconditioning prevents doxorubicin-induced dilated cardiomyopathy through an increase of antioxidant enzymes and a reduction of oxidised macromolecules. This establishes the background for future studies to determine if ozone therapy can be used as a complementary treatment for attenuating doxorubicin-induced cardiotoxicity in cancer patients.http://web.squ.edu.om/squmj/includes/tng/pub/tNG_download.asp?id=fc7e8652c33e23df03e61b5298e4ff56OzoneDoxorubicinDilated CardiomyopathyCardiotoxinsOxidative Stress |
| spellingShingle | Livan Delgado-Roche Yanet Hernández-Matos Emilio A. Medina Dalia Á. Morejón Maité R. González Gregorio Martίnez-Sánchez Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats Sultan Qaboos University Medical Journal Ozone Doxorubicin Dilated Cardiomyopathy Cardiotoxins Oxidative Stress |
| title | Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats |
| title_full | Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats |
| title_fullStr | Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats |
| title_full_unstemmed | Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats |
| title_short | Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats |
| title_sort | ozone oxidative preconditioning prevents doxorubicin induced cardiotoxicity in sprague dawley rats |
| topic | Ozone Doxorubicin Dilated Cardiomyopathy Cardiotoxins Oxidative Stress |
| url | http://web.squ.edu.om/squmj/includes/tng/pub/tNG_download.asp?id=fc7e8652c33e23df03e61b5298e4ff56 |
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