Evaluation of anticancer activity of water and juice extracts of young <i>Hordeum vulgare</i> in human cancer cell lines HT-29 and A549
Introduction and objective Barley (<i>Hordeum vulgare L.</i>) is known as a rich source of different bioactive compounds. At present, considerable attention of researchers is focused on young barley grass. It can be a good source of dietary minerals, vitamins, carbohydrates, amino acids,...
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| Format: | Article |
| Language: | English |
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Institute of Rural Health
2017-06-01
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| Series: | Annals of Agricultural and Environmental Medicine |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/aaem/Evaluation-of-anticancer-activity-of-water-and-juice-extracts-of-young-Hordeum-vulgare-in-human-cancer-cell-lines-HT-29-and-A549,74714,0,2.html |
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| author | Arkadiusz Czerwonka Katarzyna Kawka Klaudia Cykier Marta Kinga Lemieszek Wojciech Rzeski |
| author_facet | Arkadiusz Czerwonka Katarzyna Kawka Klaudia Cykier Marta Kinga Lemieszek Wojciech Rzeski |
| author_sort | Arkadiusz Czerwonka |
| collection | DOAJ |
| description | Introduction and objective
Barley (<i>Hordeum vulgare L.</i>) is known as a rich source of different bioactive compounds. At present, considerable attention of researchers is focused on young barley grass. It can be a good source of dietary minerals, vitamins, carbohydrates, amino acids, phenolic compounds and proteins. It is possible that the composition of chemical ingredients beneficial for health may induce an anticancer potential of young barley in human colon adenocarcinoma (HT-29) and human lung adenocarcinoma (A549) cell lines.
Material and Methods
<i>Hordeum vulgare</i> water extract (HWE) and Hordeum vulgare juice extract (HJE) were prepared. Cell proliferation and viability were examined with the use of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and NR (neutral red) methods. Induction of necrosis was assessed by propidium iodide/Hoechst staining. Progress of the cell cycle involved in cell proliferation, apoptosis, and regulation of transcription was estimated using flow cytometry analysis. Additionally, the capability of free radical scavenging was evaluated with the DPPH assay.
Results
The study revealed that extracts inhibited the proliferation of cancer cells. The NR study confirmed the low cytotoxic activity of the tested extracts to normal human colon epithelial cells (CCD 841 CoTr) and human skin fibroblasts (HSF). Furthermore, a dose-dependent cytotoxicity against HT-29 cells, but not A549 cells, has been reported. The free radical scavenging activity was observed in the case of the HWE but not the HJE.
Conclusions
The obtained results indicate a cancer chemopreventive potential of young barley as a safe dietary agent in
colon carcinoma. |
| first_indexed | 2024-04-13T02:17:44Z |
| format | Article |
| id | doaj.art-1a4d0fbc808d4adbbc4c9c4396e45160 |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-04-13T02:17:44Z |
| publishDate | 2017-06-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-1a4d0fbc808d4adbbc4c9c4396e451602022-12-22T03:07:05ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632017-06-0124234534910.26444/aaem/7471474714Evaluation of anticancer activity of water and juice extracts of young <i>Hordeum vulgare</i> in human cancer cell lines HT-29 and A549Arkadiusz Czerwonka0Katarzyna Kawka1Klaudia Cykier2Marta Kinga Lemieszek3Wojciech Rzeski4Departament of Virology and Immunology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska UniversityDepartament of Virology and Immunology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska UniversityDepartament of Virology and Immunology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska UniversityDepartment of Medical Biology, Institute of Rural Health, Lublin, PolandDepartament of Virology and Immunology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska UniversityIntroduction and objective Barley (<i>Hordeum vulgare L.</i>) is known as a rich source of different bioactive compounds. At present, considerable attention of researchers is focused on young barley grass. It can be a good source of dietary minerals, vitamins, carbohydrates, amino acids, phenolic compounds and proteins. It is possible that the composition of chemical ingredients beneficial for health may induce an anticancer potential of young barley in human colon adenocarcinoma (HT-29) and human lung adenocarcinoma (A549) cell lines. Material and Methods <i>Hordeum vulgare</i> water extract (HWE) and Hordeum vulgare juice extract (HJE) were prepared. Cell proliferation and viability were examined with the use of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and NR (neutral red) methods. Induction of necrosis was assessed by propidium iodide/Hoechst staining. Progress of the cell cycle involved in cell proliferation, apoptosis, and regulation of transcription was estimated using flow cytometry analysis. Additionally, the capability of free radical scavenging was evaluated with the DPPH assay. Results The study revealed that extracts inhibited the proliferation of cancer cells. The NR study confirmed the low cytotoxic activity of the tested extracts to normal human colon epithelial cells (CCD 841 CoTr) and human skin fibroblasts (HSF). Furthermore, a dose-dependent cytotoxicity against HT-29 cells, but not A549 cells, has been reported. The free radical scavenging activity was observed in the case of the HWE but not the HJE. Conclusions The obtained results indicate a cancer chemopreventive potential of young barley as a safe dietary agent in colon carcinoma.http://www.journalssystem.com/aaem/Evaluation-of-anticancer-activity-of-water-and-juice-extracts-of-young-Hordeum-vulgare-in-human-cancer-cell-lines-HT-29-and-A549,74714,0,2.htmlHordeum vulgarecolon cancerlung cancerinhibition of proliferationnecrosis |
| spellingShingle | Arkadiusz Czerwonka Katarzyna Kawka Klaudia Cykier Marta Kinga Lemieszek Wojciech Rzeski Evaluation of anticancer activity of water and juice extracts of young <i>Hordeum vulgare</i> in human cancer cell lines HT-29 and A549 Annals of Agricultural and Environmental Medicine Hordeum vulgare colon cancer lung cancer inhibition of proliferation necrosis |
| title | Evaluation of anticancer activity of water and juice extracts of young <i>Hordeum vulgare</i> in human cancer cell lines HT-29 and A549 |
| title_full | Evaluation of anticancer activity of water and juice extracts of young <i>Hordeum vulgare</i> in human cancer cell lines HT-29 and A549 |
| title_fullStr | Evaluation of anticancer activity of water and juice extracts of young <i>Hordeum vulgare</i> in human cancer cell lines HT-29 and A549 |
| title_full_unstemmed | Evaluation of anticancer activity of water and juice extracts of young <i>Hordeum vulgare</i> in human cancer cell lines HT-29 and A549 |
| title_short | Evaluation of anticancer activity of water and juice extracts of young <i>Hordeum vulgare</i> in human cancer cell lines HT-29 and A549 |
| title_sort | evaluation of anticancer activity of water and juice extracts of young i hordeum vulgare i in human cancer cell lines ht 29 and a549 |
| topic | Hordeum vulgare colon cancer lung cancer inhibition of proliferation necrosis |
| url | http://www.journalssystem.com/aaem/Evaluation-of-anticancer-activity-of-water-and-juice-extracts-of-young-Hordeum-vulgare-in-human-cancer-cell-lines-HT-29-and-A549,74714,0,2.html |
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