Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies
Photoaging is an important extrinsic aging factor leading to altered skin morphology and reduced function. Prior work has revealed a connection between photoaging and loss of subcutaneous fat. Currently, primary models for studying this are in vivo (human samples or animal models) or in vitro models...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/12/12/1828 |
| _version_ | 1827641514016112640 |
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| author | Martina M. Sanchez Thamidul Islam Tonmoy B. Hyle Park Joshua T. Morgan |
| author_facet | Martina M. Sanchez Thamidul Islam Tonmoy B. Hyle Park Joshua T. Morgan |
| author_sort | Martina M. Sanchez |
| collection | DOAJ |
| description | Photoaging is an important extrinsic aging factor leading to altered skin morphology and reduced function. Prior work has revealed a connection between photoaging and loss of subcutaneous fat. Currently, primary models for studying this are in vivo (human samples or animal models) or in vitro models, including human skin equivalents (HSEs). In vivo models are limited by accessibility and cost, while HSEs typically do not include a subcutaneous adipose component. To address this, we developed an “adipose-vascular” HSE (AVHSE) culture method, which includes both hypodermal adipose and vascular cells. Furthermore, we tested AVHSE as a potential model for hypodermal adipose aging via exposure to 0.45 ± 0.15 mW/cm<sup>2</sup> 385 nm light (UVA). One week of 2 h daily UVA exposure had limited impact on epidermal and vascular components of the AVHSE, but significantly reduced adiposity by approximately 50%. Overall, we have developed a novel method for generating HSE that include vascular and adipose components and demonstrated potential as an aging model using photoaging as an example. |
| first_indexed | 2024-03-09T17:15:57Z |
| format | Article |
| id | doaj.art-1a4d48020e854b0e8832ae38c37f13e9 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T17:15:57Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-1a4d48020e854b0e8832ae38c37f13e92023-11-24T13:34:03ZengMDPI AGBiomolecules2218-273X2022-12-011212182810.3390/biom12121828Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging StudiesMartina M. Sanchez0Thamidul Islam Tonmoy1B. Hyle Park2Joshua T. Morgan3Department of Bioengineering, University of California, Riverside, CA 92521, USADepartment of Bioengineering, University of California, Riverside, CA 92521, USADepartment of Bioengineering, University of California, Riverside, CA 92521, USADepartment of Bioengineering, University of California, Riverside, CA 92521, USAPhotoaging is an important extrinsic aging factor leading to altered skin morphology and reduced function. Prior work has revealed a connection between photoaging and loss of subcutaneous fat. Currently, primary models for studying this are in vivo (human samples or animal models) or in vitro models, including human skin equivalents (HSEs). In vivo models are limited by accessibility and cost, while HSEs typically do not include a subcutaneous adipose component. To address this, we developed an “adipose-vascular” HSE (AVHSE) culture method, which includes both hypodermal adipose and vascular cells. Furthermore, we tested AVHSE as a potential model for hypodermal adipose aging via exposure to 0.45 ± 0.15 mW/cm<sup>2</sup> 385 nm light (UVA). One week of 2 h daily UVA exposure had limited impact on epidermal and vascular components of the AVHSE, but significantly reduced adiposity by approximately 50%. Overall, we have developed a novel method for generating HSE that include vascular and adipose components and demonstrated potential as an aging model using photoaging as an example.https://www.mdpi.com/2218-273X/12/12/1828HSEhuman skin equivalenttissue engineeringself-assemblyscaffoldaging |
| spellingShingle | Martina M. Sanchez Thamidul Islam Tonmoy B. Hyle Park Joshua T. Morgan Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies Biomolecules HSE human skin equivalent tissue engineering self-assembly scaffold aging |
| title | Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies |
| title_full | Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies |
| title_fullStr | Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies |
| title_full_unstemmed | Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies |
| title_short | Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies |
| title_sort | development of a vascularized human skin equivalent with hypodermis for photoaging studies |
| topic | HSE human skin equivalent tissue engineering self-assembly scaffold aging |
| url | https://www.mdpi.com/2218-273X/12/12/1828 |
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