Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity
Abstract Background A rational and high-performance high-throughput screening system effectively improves the efficiency of strain screening. Results A rapid and efficient method was developed to detect carbon sources. And then by using glucose analog as screening pressure and two indexes for sophor...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-05-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40643-019-0252-x |
| _version_ | 1818442220698599424 |
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| author | Yumeng Lin Yang Chen Qianhui Li Xiwei Tian Ju Chu |
| author_facet | Yumeng Lin Yang Chen Qianhui Li Xiwei Tian Ju Chu |
| author_sort | Yumeng Lin |
| collection | DOAJ |
| description | Abstract Background A rational and high-performance high-throughput screening system effectively improves the efficiency of strain screening. Results A rapid and efficient method was developed to detect carbon sources. And then by using glucose analog as screening pressure and two indexes for sophorolipids (SLs) production and glycerol consumption, a mutant of Candida bombicola L1.3 was successfully selected among 3000 mutants. It produces high SLs titer and co-utilizes glucose and glycerol simultaneously. Conclusions Compared to the wild-type strain, L1.3 exhibited 15.06% higher SLs titer and 35.69% higher glycerol consumption capacity in a 5-L bioreactor. We believe that L1.3 can potentially be used for the efficient industrial production of SLs with simple downstream processing for separating SLs and glycerol. |
| first_indexed | 2024-12-14T18:40:41Z |
| format | Article |
| id | doaj.art-1a4e4e1d4d204fe6a09d6c7d492b5569 |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-14T18:40:41Z |
| publishDate | 2019-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-1a4e4e1d4d204fe6a09d6c7d492b55692022-12-21T22:51:30ZengSpringerOpenBioresources and Bioprocessing2197-43652019-05-01611910.1186/s40643-019-0252-xRational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacityYumeng Lin0Yang Chen1Qianhui Li2Xiwei Tian3Ju Chu4State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyAbstract Background A rational and high-performance high-throughput screening system effectively improves the efficiency of strain screening. Results A rapid and efficient method was developed to detect carbon sources. And then by using glucose analog as screening pressure and two indexes for sophorolipids (SLs) production and glycerol consumption, a mutant of Candida bombicola L1.3 was successfully selected among 3000 mutants. It produces high SLs titer and co-utilizes glucose and glycerol simultaneously. Conclusions Compared to the wild-type strain, L1.3 exhibited 15.06% higher SLs titer and 35.69% higher glycerol consumption capacity in a 5-L bioreactor. We believe that L1.3 can potentially be used for the efficient industrial production of SLs with simple downstream processing for separating SLs and glycerol.http://link.springer.com/article/10.1186/s40643-019-0252-xHigh-throughput screeningGlycerolSophorolipids2-Deoxy-d-glucose |
| spellingShingle | Yumeng Lin Yang Chen Qianhui Li Xiwei Tian Ju Chu Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity Bioresources and Bioprocessing High-throughput screening Glycerol Sophorolipids 2-Deoxy-d-glucose |
| title | Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity |
| title_full | Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity |
| title_fullStr | Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity |
| title_full_unstemmed | Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity |
| title_short | Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity |
| title_sort | rational high throughput screening system for high sophorolipids production in candida bombicola by co utilizing glycerol and glucose capacity |
| topic | High-throughput screening Glycerol Sophorolipids 2-Deoxy-d-glucose |
| url | http://link.springer.com/article/10.1186/s40643-019-0252-x |
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