Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proof-of-concept study
When nanoparticles were introduced into the biological media, the protein corona would be formed, which endowed the nanoparticles with new bio-identities. Thus, controlling protein corona formation is critical to in vivo therapeutic effect. Controlling the particle size is the most feasible method d...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-04-01
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| Series: | Acta Pharmaceutica Sinica B |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211383520307759 |
| _version_ | 1819133859309027328 |
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| author | Wenhao Wang Zhengwei Huang Yanbei Li Wenhua Wang Jiayu Shi Fangqin Fu Ying Huang Xin Pan Chuanbin Wu |
| author_facet | Wenhao Wang Zhengwei Huang Yanbei Li Wenhua Wang Jiayu Shi Fangqin Fu Ying Huang Xin Pan Chuanbin Wu |
| author_sort | Wenhao Wang |
| collection | DOAJ |
| description | When nanoparticles were introduced into the biological media, the protein corona would be formed, which endowed the nanoparticles with new bio-identities. Thus, controlling protein corona formation is critical to in vivo therapeutic effect. Controlling the particle size is the most feasible method during design, and the influence of media pH which varies with disease condition is quite important. The impact of particle size and pH on bovine serum albumin (BSA) corona formation of solid lipid nanoparticles (SLNs) was studied here. The BSA corona formation of SLNs with increasing particle size (120–480 nm) in pH 6.0 and 7.4 was investigated. Multiple techniques were employed for visualization study, conformational structure study and mechanism study, etc. “BSA corona-caused aggregation” of SLN2‒3 was revealed in pH 6.0 while the dispersed state of SLNs was maintained in pH 7.4, which significantly affected the secondary structure of BSA and cell uptake of SLNs. The main interaction was driven by van der Waals force plus hydrogen bonding in pH 7.4, while by electrostatic attraction in pH 6.0, and size-dependent adsorption was confirmed. This study provides a systematic insight to the understanding of protein corona formation of SLNs. |
| first_indexed | 2024-12-22T09:53:59Z |
| format | Article |
| id | doaj.art-1a4ee05b649b4a85834328f9026f4927 |
| institution | Directory Open Access Journal |
| issn | 2211-3835 |
| language | English |
| last_indexed | 2024-12-22T09:53:59Z |
| publishDate | 2021-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj.art-1a4ee05b649b4a85834328f9026f49272022-12-21T18:30:19ZengElsevierActa Pharmaceutica Sinica B2211-38352021-04-0111410301046Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proof-of-concept studyWenhao Wang0Zhengwei Huang1Yanbei Li2Wenhua Wang3Jiayu Shi4Fangqin Fu5Ying Huang6Xin Pan7Chuanbin Wu8School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, ChinaCollege of Pharmacy, Jinan University, Guangzhou 511443, ChinaCollege of Pharmacy, Jinan University, Guangzhou 511443, China; Corresponding authors. Tel.: +86 20 39943427, fax: +86 020 39943115 (Ying Huang); Tel./fax: +86 20 39943120 (Chuanbin Wu).School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China; Corresponding authors. Tel.: +86 20 39943427, fax: +86 020 39943115 (Ying Huang); Tel./fax: +86 20 39943120 (Chuanbin Wu).When nanoparticles were introduced into the biological media, the protein corona would be formed, which endowed the nanoparticles with new bio-identities. Thus, controlling protein corona formation is critical to in vivo therapeutic effect. Controlling the particle size is the most feasible method during design, and the influence of media pH which varies with disease condition is quite important. The impact of particle size and pH on bovine serum albumin (BSA) corona formation of solid lipid nanoparticles (SLNs) was studied here. The BSA corona formation of SLNs with increasing particle size (120–480 nm) in pH 6.0 and 7.4 was investigated. Multiple techniques were employed for visualization study, conformational structure study and mechanism study, etc. “BSA corona-caused aggregation” of SLN2‒3 was revealed in pH 6.0 while the dispersed state of SLNs was maintained in pH 7.4, which significantly affected the secondary structure of BSA and cell uptake of SLNs. The main interaction was driven by van der Waals force plus hydrogen bonding in pH 7.4, while by electrostatic attraction in pH 6.0, and size-dependent adsorption was confirmed. This study provides a systematic insight to the understanding of protein corona formation of SLNs.http://www.sciencedirect.com/science/article/pii/S2211383520307759Protein coronaSolid lipid nanoparticlesBSA corona-Caused aggregationNanoparticle-protein interactionSize effectCell uptake |
| spellingShingle | Wenhao Wang Zhengwei Huang Yanbei Li Wenhua Wang Jiayu Shi Fangqin Fu Ying Huang Xin Pan Chuanbin Wu Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proof-of-concept study Acta Pharmaceutica Sinica B Protein corona Solid lipid nanoparticles BSA corona-Caused aggregation Nanoparticle-protein interaction Size effect Cell uptake |
| title | Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proof-of-concept study |
| title_full | Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proof-of-concept study |
| title_fullStr | Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proof-of-concept study |
| title_full_unstemmed | Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proof-of-concept study |
| title_short | Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proof-of-concept study |
| title_sort | impact of particle size and ph on protein corona formation of solid lipid nanoparticles a proof of concept study |
| topic | Protein corona Solid lipid nanoparticles BSA corona-Caused aggregation Nanoparticle-protein interaction Size effect Cell uptake |
| url | http://www.sciencedirect.com/science/article/pii/S2211383520307759 |
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