Genetic variation in hydrogen cyanide potential of perennial sorghum evaluated by colorimetry
Abstract Both annual and perennial sorghum biomass serve as important forage for ruminant animals around the world. Unfortunately, sorghum can produce hydrogen cyanide (HCN), which, if occurring in high enough concentrations, can be toxic or lethal to animals that consume it. The objectives of this...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-10-01
|
| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.448 |
| _version_ | 1811198425169920000 |
|---|---|
| author | Shakirah Nakasagga Seth C. Murray William L. Rooney Catherine Barr Pheona Nabukalu Stan Cox Leo Hoffmann Jr. |
| author_facet | Shakirah Nakasagga Seth C. Murray William L. Rooney Catherine Barr Pheona Nabukalu Stan Cox Leo Hoffmann Jr. |
| author_sort | Shakirah Nakasagga |
| collection | DOAJ |
| description | Abstract Both annual and perennial sorghum biomass serve as important forage for ruminant animals around the world. Unfortunately, sorghum can produce hydrogen cyanide (HCN), which, if occurring in high enough concentrations, can be toxic or lethal to animals that consume it. The objectives of this study were to develop a fast and inexpensive colorimetric assay to measure the hydrogen cyanide potential (HCN‐P) as well as to compare this with existing visual assays while assessing the range of variation for HCN‐P among perennial and annual sorghum biomass. The HCN‐P of 100 sorghum lines derived from an interspecific hybridization program was determined over 2 years (establishment and regrowth) using both visual and colorimetric assays. Visual assessment underestimated the HCN‐P and was less accurate than colorimetry. Repeatability for HCN‐P across all sampling dates was functionally zero in the visual assessment and low for the colorimetric assay. This was mostly explained by the significant pedigree × year interaction effects and growth stage. Growth stage substantially influenced HCN‐P, which should be considered when feeding animals on fresh forage. |
| first_indexed | 2024-04-12T01:31:25Z |
| format | Article |
| id | doaj.art-1a59c440b9c3444cb6857c04cd6cf5e9 |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-04-12T01:31:25Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-1a59c440b9c3444cb6857c04cd6cf5e92022-12-22T03:53:28ZengWileyPlant Direct2475-44552022-10-01610n/an/a10.1002/pld3.448Genetic variation in hydrogen cyanide potential of perennial sorghum evaluated by colorimetryShakirah Nakasagga0Seth C. Murray1William L. Rooney2Catherine Barr3Pheona Nabukalu4Stan Cox5Leo Hoffmann Jr.6Department of Soil and Crop Sciences Texas A&M University College Station Texas USADepartment of Soil and Crop Sciences Texas A&M University College Station Texas USADepartment of Soil and Crop Sciences Texas A&M University College Station Texas USATexas A&M Veterinary Medical Diagnostic Laboratory College Station Texas USAThe Land Institute Salina Kansas USAThe Land Institute Salina Kansas USADepartment of Horticulture Sciences University of Florida Gainesville Florida USAAbstract Both annual and perennial sorghum biomass serve as important forage for ruminant animals around the world. Unfortunately, sorghum can produce hydrogen cyanide (HCN), which, if occurring in high enough concentrations, can be toxic or lethal to animals that consume it. The objectives of this study were to develop a fast and inexpensive colorimetric assay to measure the hydrogen cyanide potential (HCN‐P) as well as to compare this with existing visual assays while assessing the range of variation for HCN‐P among perennial and annual sorghum biomass. The HCN‐P of 100 sorghum lines derived from an interspecific hybridization program was determined over 2 years (establishment and regrowth) using both visual and colorimetric assays. Visual assessment underestimated the HCN‐P and was less accurate than colorimetry. Repeatability for HCN‐P across all sampling dates was functionally zero in the visual assessment and low for the colorimetric assay. This was mostly explained by the significant pedigree × year interaction effects and growth stage. Growth stage substantially influenced HCN‐P, which should be considered when feeding animals on fresh forage.https://doi.org/10.1002/pld3.448colorimetryhydrogen cyanide potentialperennial sorghumvisual assessment |
| spellingShingle | Shakirah Nakasagga Seth C. Murray William L. Rooney Catherine Barr Pheona Nabukalu Stan Cox Leo Hoffmann Jr. Genetic variation in hydrogen cyanide potential of perennial sorghum evaluated by colorimetry Plant Direct colorimetry hydrogen cyanide potential perennial sorghum visual assessment |
| title | Genetic variation in hydrogen cyanide potential of perennial sorghum evaluated by colorimetry |
| title_full | Genetic variation in hydrogen cyanide potential of perennial sorghum evaluated by colorimetry |
| title_fullStr | Genetic variation in hydrogen cyanide potential of perennial sorghum evaluated by colorimetry |
| title_full_unstemmed | Genetic variation in hydrogen cyanide potential of perennial sorghum evaluated by colorimetry |
| title_short | Genetic variation in hydrogen cyanide potential of perennial sorghum evaluated by colorimetry |
| title_sort | genetic variation in hydrogen cyanide potential of perennial sorghum evaluated by colorimetry |
| topic | colorimetry hydrogen cyanide potential perennial sorghum visual assessment |
| url | https://doi.org/10.1002/pld3.448 |
| work_keys_str_mv | AT shakirahnakasagga geneticvariationinhydrogencyanidepotentialofperennialsorghumevaluatedbycolorimetry AT sethcmurray geneticvariationinhydrogencyanidepotentialofperennialsorghumevaluatedbycolorimetry AT williamlrooney geneticvariationinhydrogencyanidepotentialofperennialsorghumevaluatedbycolorimetry AT catherinebarr geneticvariationinhydrogencyanidepotentialofperennialsorghumevaluatedbycolorimetry AT pheonanabukalu geneticvariationinhydrogencyanidepotentialofperennialsorghumevaluatedbycolorimetry AT stancox geneticvariationinhydrogencyanidepotentialofperennialsorghumevaluatedbycolorimetry AT leohoffmannjr geneticvariationinhydrogencyanidepotentialofperennialsorghumevaluatedbycolorimetry |