Analysis of Evolution, Expression and Genetic Transformation of TCP Transcription Factors in Blueberry Reveal That VcTCP18 Negatively Regulates the Release of Flower Bud Dormancy
Plant-specific TEOSINTE BRANCHED 1, CYCLOIDEA, PROLIFERATING CELL FACTORS (TCP) transcription factors have versatile functions in plant growth, development and response to environmental stress. Despite blueberry’s value as an important fruit crop, the TCP gene family has not been systematically stud...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-07-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.697609/full |
| _version_ | 1818728184069226496 |
|---|---|
| author | Yongqiang Li Yongqiang Li Shuang An Qiangqiang Cheng Yu Zong Wenrong Chen Weidong Guo Lu Zhang |
| author_facet | Yongqiang Li Yongqiang Li Shuang An Qiangqiang Cheng Yu Zong Wenrong Chen Weidong Guo Lu Zhang |
| author_sort | Yongqiang Li |
| collection | DOAJ |
| description | Plant-specific TEOSINTE BRANCHED 1, CYCLOIDEA, PROLIFERATING CELL FACTORS (TCP) transcription factors have versatile functions in plant growth, development and response to environmental stress. Despite blueberry’s value as an important fruit crop, the TCP gene family has not been systematically studied in this plant. The current study identified blueberry TCP genes (VcTCPs) using genomic data from the tetraploid blueberry variety ‘Draper’; a total of 62 genes were obtained. Using multiple sequence alignment, conserved motif, and gene structure analyses, family members were divided into two subfamilies, of which class II was further divided into two subclasses, CIN and TB1. Synteny analysis showed that genome-wide or segment-based replication played an important role in the expansion of the blueberry TCP gene family. The expression patterns of VcTCP genes during fruit development, flower bud dormancy release, hormone treatment, and tissue-specific expression were analyzed using RNA-seq and qRT-PCR. The results showed that the TB1 subclass members exhibited a certain level of expression in the shoot, leaf, and bud; these genes were not expressed during fruit development, but transcript levels decreased uniformly during the release of flower bud dormancy by low-temperature accumulation. The further transgenic experiments showed the overexpression of VcTCP18 in Arabidopsis significantly decreased the seed germination rate in contrast to the wild type. The bud dormancy phenomena as late-flowering, fewer rosettes and main branches were also observed in transgenic plants. Overall, this study provides the first insight into the evolution, expression, and function of VcTCP genes, including the discovery that VcTCP18 negatively regulated bud dormancy release in blueberry. The results will deepen our understanding of the function of TCPs in plant growth and development. |
| first_indexed | 2024-12-17T22:25:57Z |
| format | Article |
| id | doaj.art-1a5b0ee5806740afb5da0d51cb567675 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T22:25:57Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1a5b0ee5806740afb5da0d51cb5676752022-12-21T21:30:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-07-011210.3389/fpls.2021.697609697609Analysis of Evolution, Expression and Genetic Transformation of TCP Transcription Factors in Blueberry Reveal That VcTCP18 Negatively Regulates the Release of Flower Bud DormancyYongqiang Li0Yongqiang Li1Shuang An2Qiangqiang Cheng3Yu Zong4Wenrong Chen5Weidong Guo6Lu Zhang7Key Laboratory of Silviculture, Co-Innovation Center of Jiangxi Typical Trees Cultivation and Utilization, College of Forestry, Jiangxi Agricultural University, Nanchang, ChinaZhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, ChinaZhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, ChinaKey Laboratory of Silviculture, Co-Innovation Center of Jiangxi Typical Trees Cultivation and Utilization, College of Forestry, Jiangxi Agricultural University, Nanchang, ChinaZhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, ChinaZhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, ChinaZhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, ChinaKey Laboratory of Silviculture, Co-Innovation Center of Jiangxi Typical Trees Cultivation and Utilization, College of Forestry, Jiangxi Agricultural University, Nanchang, ChinaPlant-specific TEOSINTE BRANCHED 1, CYCLOIDEA, PROLIFERATING CELL FACTORS (TCP) transcription factors have versatile functions in plant growth, development and response to environmental stress. Despite blueberry’s value as an important fruit crop, the TCP gene family has not been systematically studied in this plant. The current study identified blueberry TCP genes (VcTCPs) using genomic data from the tetraploid blueberry variety ‘Draper’; a total of 62 genes were obtained. Using multiple sequence alignment, conserved motif, and gene structure analyses, family members were divided into two subfamilies, of which class II was further divided into two subclasses, CIN and TB1. Synteny analysis showed that genome-wide or segment-based replication played an important role in the expansion of the blueberry TCP gene family. The expression patterns of VcTCP genes during fruit development, flower bud dormancy release, hormone treatment, and tissue-specific expression were analyzed using RNA-seq and qRT-PCR. The results showed that the TB1 subclass members exhibited a certain level of expression in the shoot, leaf, and bud; these genes were not expressed during fruit development, but transcript levels decreased uniformly during the release of flower bud dormancy by low-temperature accumulation. The further transgenic experiments showed the overexpression of VcTCP18 in Arabidopsis significantly decreased the seed germination rate in contrast to the wild type. The bud dormancy phenomena as late-flowering, fewer rosettes and main branches were also observed in transgenic plants. Overall, this study provides the first insight into the evolution, expression, and function of VcTCP genes, including the discovery that VcTCP18 negatively regulated bud dormancy release in blueberry. The results will deepen our understanding of the function of TCPs in plant growth and development.https://www.frontiersin.org/articles/10.3389/fpls.2021.697609/fullblueberryTCP transcription factorsexpression profiles analysisflower bud dormancytransgenic plants |
| spellingShingle | Yongqiang Li Yongqiang Li Shuang An Qiangqiang Cheng Yu Zong Wenrong Chen Weidong Guo Lu Zhang Analysis of Evolution, Expression and Genetic Transformation of TCP Transcription Factors in Blueberry Reveal That VcTCP18 Negatively Regulates the Release of Flower Bud Dormancy Frontiers in Plant Science blueberry TCP transcription factors expression profiles analysis flower bud dormancy transgenic plants |
| title | Analysis of Evolution, Expression and Genetic Transformation of TCP Transcription Factors in Blueberry Reveal That VcTCP18 Negatively Regulates the Release of Flower Bud Dormancy |
| title_full | Analysis of Evolution, Expression and Genetic Transformation of TCP Transcription Factors in Blueberry Reveal That VcTCP18 Negatively Regulates the Release of Flower Bud Dormancy |
| title_fullStr | Analysis of Evolution, Expression and Genetic Transformation of TCP Transcription Factors in Blueberry Reveal That VcTCP18 Negatively Regulates the Release of Flower Bud Dormancy |
| title_full_unstemmed | Analysis of Evolution, Expression and Genetic Transformation of TCP Transcription Factors in Blueberry Reveal That VcTCP18 Negatively Regulates the Release of Flower Bud Dormancy |
| title_short | Analysis of Evolution, Expression and Genetic Transformation of TCP Transcription Factors in Blueberry Reveal That VcTCP18 Negatively Regulates the Release of Flower Bud Dormancy |
| title_sort | analysis of evolution expression and genetic transformation of tcp transcription factors in blueberry reveal that vctcp18 negatively regulates the release of flower bud dormancy |
| topic | blueberry TCP transcription factors expression profiles analysis flower bud dormancy transgenic plants |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.697609/full |
| work_keys_str_mv | AT yongqiangli analysisofevolutionexpressionandgenetictransformationoftcptranscriptionfactorsinblueberryrevealthatvctcp18negativelyregulatesthereleaseofflowerbuddormancy AT yongqiangli analysisofevolutionexpressionandgenetictransformationoftcptranscriptionfactorsinblueberryrevealthatvctcp18negativelyregulatesthereleaseofflowerbuddormancy AT shuangan analysisofevolutionexpressionandgenetictransformationoftcptranscriptionfactorsinblueberryrevealthatvctcp18negativelyregulatesthereleaseofflowerbuddormancy AT qiangqiangcheng analysisofevolutionexpressionandgenetictransformationoftcptranscriptionfactorsinblueberryrevealthatvctcp18negativelyregulatesthereleaseofflowerbuddormancy AT yuzong analysisofevolutionexpressionandgenetictransformationoftcptranscriptionfactorsinblueberryrevealthatvctcp18negativelyregulatesthereleaseofflowerbuddormancy AT wenrongchen analysisofevolutionexpressionandgenetictransformationoftcptranscriptionfactorsinblueberryrevealthatvctcp18negativelyregulatesthereleaseofflowerbuddormancy AT weidongguo analysisofevolutionexpressionandgenetictransformationoftcptranscriptionfactorsinblueberryrevealthatvctcp18negativelyregulatesthereleaseofflowerbuddormancy AT luzhang analysisofevolutionexpressionandgenetictransformationoftcptranscriptionfactorsinblueberryrevealthatvctcp18negativelyregulatesthereleaseofflowerbuddormancy |