Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen
AIM: To investigate the effects of collagen and opticin on the bioactivity of human retinal vascular endothelial cells (hRVECs), and explore its regulations by integrins and RhoA/ROCK1 signal pathway. METHODS: hRVECs were cultured in collagen and treated by opticin, and cell-based bioactivity assay...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2020-08-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/en_publish/2020/8/20200805.pdf |
| _version_ | 1818518434911092736 |
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| author | Yue Xing Xue-Ke Li Si-Duo Lu Jin Ma |
| author_facet | Yue Xing Xue-Ke Li Si-Duo Lu Jin Ma |
| author_sort | Yue Xing |
| collection | DOAJ |
| description | AIM: To investigate the effects of collagen and opticin on the bioactivity of human retinal vascular endothelial cells (hRVECs), and explore its regulations by integrins and RhoA/ROCK1 signal pathway.
METHODS: hRVECs were cultured in collagen and treated by opticin, and cell-based bioactivity assays of cell proliferation, migration, and adhesion were performed. The expression of integrin α2, integrin β1, RhoA and ROCK1 were examined with real-time PCR and Western blotting.
RESULTS: Collagen could promote cell viability of proliferation and migration (all P<0.05), and enhance the mRNA expression of integrin α2, integrin β1, RhoA and ROCK1 (all P<0.05). Opticin could inhibit proliferation and migration ability of hRVECs cultured in collagen, and reduce the mRNA expression of integrin α2, integrin β1, RhoA and ROCK1 (all P<0.05).
CONCLUSION: Collagen and opticin can affect bioactivity of hRVECs, which may be regulated by α2-, β1-integrins and RhoA/ROCK1 signal pathway. |
| first_indexed | 2024-12-11T01:10:30Z |
| format | Article |
| id | doaj.art-1a5d3f9d64b447469444456c25f5fb42 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-11T01:10:30Z |
| publishDate | 2020-08-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-1a5d3f9d64b447469444456c25f5fb422022-12-22T01:26:03ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982020-08-011381202120910.18240/ijo.2020.08.05Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagenYue Xing0Xue-Ke Li1Si-Duo Lu2Jin Ma3State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510000, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510000, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510000, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510000, Guangdong Province, ChinaAIM: To investigate the effects of collagen and opticin on the bioactivity of human retinal vascular endothelial cells (hRVECs), and explore its regulations by integrins and RhoA/ROCK1 signal pathway. METHODS: hRVECs were cultured in collagen and treated by opticin, and cell-based bioactivity assays of cell proliferation, migration, and adhesion were performed. The expression of integrin α2, integrin β1, RhoA and ROCK1 were examined with real-time PCR and Western blotting. RESULTS: Collagen could promote cell viability of proliferation and migration (all P<0.05), and enhance the mRNA expression of integrin α2, integrin β1, RhoA and ROCK1 (all P<0.05). Opticin could inhibit proliferation and migration ability of hRVECs cultured in collagen, and reduce the mRNA expression of integrin α2, integrin β1, RhoA and ROCK1 (all P<0.05). CONCLUSION: Collagen and opticin can affect bioactivity of hRVECs, which may be regulated by α2-, β1-integrins and RhoA/ROCK1 signal pathway.http://ies.ijo.cn/en_publish/2020/8/20200805.pdfopticincollagenretinal vascular endothelial cellintegrinrhoa/rock1 |
| spellingShingle | Yue Xing Xue-Ke Li Si-Duo Lu Jin Ma Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen International Journal of Ophthalmology opticin collagen retinal vascular endothelial cell integrin rhoa/rock1 |
| title | Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen |
| title_full | Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen |
| title_fullStr | Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen |
| title_full_unstemmed | Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen |
| title_short | Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen |
| title_sort | regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen |
| topic | opticin collagen retinal vascular endothelial cell integrin rhoa/rock1 |
| url | http://ies.ijo.cn/en_publish/2020/8/20200805.pdf |
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