Rescue of photoreceptor with human mesenchyme stem cell and human mesenchyme stem cell expressing erythropoietin in total degeneration of retina animal model
Purpose: This study aimed to investigate the efficacy of human-derived umbilical cord mesenchymal stem cells (HDUMSC) and human-derived umbilical cord mesenchymal stem cells expressing erythropoietin (HDUMSC-EPO) to rescue total degenerated retina in a rat model. Methods: The study included four tre...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2022-01-01
|
| Series: | Indian Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.in/article.asp?issn=0301-4738;year=2022;volume=70;issue=3;spage=921;epage=929;aulast=Abd |
| _version_ | 1818906529841020928 |
|---|---|
| author | Munirah Abd Rashid Lam Chenshen Avin Koh Ee Hwan Hiba AlSaeedy Pooi Ling Mok Suresh Kumar Subbiah Hazlita Md Isa Then Kong Yong Mohd Hairul Nizam Harun Syed Mohamed Suhail B S Mohamad Angela Ng Min Hwei Chi D Luu Mae-Lynn Catherine Bastion |
| author_facet | Munirah Abd Rashid Lam Chenshen Avin Koh Ee Hwan Hiba AlSaeedy Pooi Ling Mok Suresh Kumar Subbiah Hazlita Md Isa Then Kong Yong Mohd Hairul Nizam Harun Syed Mohamed Suhail B S Mohamad Angela Ng Min Hwei Chi D Luu Mae-Lynn Catherine Bastion |
| author_sort | Munirah Abd Rashid |
| collection | DOAJ |
| description | Purpose: This study aimed to investigate the efficacy of human-derived umbilical cord mesenchymal stem cells (HDUMSC) and human-derived umbilical cord mesenchymal stem cells expressing erythropoietin (HDUMSC-EPO) to rescue total degenerated retina in a rat model. Methods: The study included four treatment groups, namely negative control using normal saline (HBSS) injection, positive control using sodium iodide 60 mg/kg (SI), SI treated with HDUMSC, and SI treated with HDUMSC-EPO given via subretinal and intravenous routes, to test the efficacy of retinal regeneration following SI-induced retinal degeneration. Retinal function in both phases was tested via electroretinography (ERG) and histological staining examining the outer nuclear layer (ONL). Results: There was a statistically significant result (P < 0.05) in the SI treated with HDUMSC-EPO only when comparing day 11 (mean = 23.6 μv), day 18 (mean = 25.2 μv), day 26 (mean = 26.3 μv), and day 32 (mean = 28.2 μv) to the b-wave ERG on day 4 rescue injection day (mean = 12.5 μv). The SI treated with HDUMSC-EPO showed significant improvement in b-wave ERG readings in the Sprague–Dawley (SD) rat but did not restore baseline readings prior to degeneration (day 0). Both treated groups' ONL thicknesses did not show significant changes compared to the negative control group (HBSS) following rescue therapy. Conclusion: Total retinal degeneration following intravenous SI injection was observed at 60 mg/kg. SI treated with HDUMSC and HDUMSC-EPO showed no regenerative potential compared to baseline in SI-induced total retina degeneration on ERG or histology, whereas SI treated with HDUMSC-EPO group showed a substantial increase in b-wave ERG amplitude over time. |
| first_indexed | 2024-12-19T21:40:41Z |
| format | Article |
| id | doaj.art-1a5e1dccf3fd48f584aefa50cc08189d |
| institution | Directory Open Access Journal |
| issn | 0301-4738 1998-3689 |
| language | English |
| last_indexed | 2024-12-19T21:40:41Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Ophthalmology |
| spelling | doaj.art-1a5e1dccf3fd48f584aefa50cc08189d2022-12-21T20:04:40ZengWolters Kluwer Medknow PublicationsIndian Journal of Ophthalmology0301-47381998-36892022-01-0170392192910.4103/ijo.IJO_472_21Rescue of photoreceptor with human mesenchyme stem cell and human mesenchyme stem cell expressing erythropoietin in total degeneration of retina animal modelMunirah Abd RashidLam ChenshenAvin Koh Ee HwanHiba AlSaeedyPooi Ling MokSuresh Kumar SubbiahHazlita Md IsaThen Kong YongMohd Hairul Nizam HarunSyed Mohamed Suhail B S MohamadAngela Ng Min HweiChi D LuuMae-Lynn Catherine BastionPurpose: This study aimed to investigate the efficacy of human-derived umbilical cord mesenchymal stem cells (HDUMSC) and human-derived umbilical cord mesenchymal stem cells expressing erythropoietin (HDUMSC-EPO) to rescue total degenerated retina in a rat model. Methods: The study included four treatment groups, namely negative control using normal saline (HBSS) injection, positive control using sodium iodide 60 mg/kg (SI), SI treated with HDUMSC, and SI treated with HDUMSC-EPO given via subretinal and intravenous routes, to test the efficacy of retinal regeneration following SI-induced retinal degeneration. Retinal function in both phases was tested via electroretinography (ERG) and histological staining examining the outer nuclear layer (ONL). Results: There was a statistically significant result (P < 0.05) in the SI treated with HDUMSC-EPO only when comparing day 11 (mean = 23.6 μv), day 18 (mean = 25.2 μv), day 26 (mean = 26.3 μv), and day 32 (mean = 28.2 μv) to the b-wave ERG on day 4 rescue injection day (mean = 12.5 μv). The SI treated with HDUMSC-EPO showed significant improvement in b-wave ERG readings in the Sprague–Dawley (SD) rat but did not restore baseline readings prior to degeneration (day 0). Both treated groups' ONL thicknesses did not show significant changes compared to the negative control group (HBSS) following rescue therapy. Conclusion: Total retinal degeneration following intravenous SI injection was observed at 60 mg/kg. SI treated with HDUMSC and HDUMSC-EPO showed no regenerative potential compared to baseline in SI-induced total retina degeneration on ERG or histology, whereas SI treated with HDUMSC-EPO group showed a substantial increase in b-wave ERG amplitude over time.http://www.ijo.in/article.asp?issn=0301-4738;year=2022;volume=70;issue=3;spage=921;epage=929;aulast=Abdelectroretinographyhdumschdumsc-epoonlretinal degenerative diseasesprague–dawley rat |
| spellingShingle | Munirah Abd Rashid Lam Chenshen Avin Koh Ee Hwan Hiba AlSaeedy Pooi Ling Mok Suresh Kumar Subbiah Hazlita Md Isa Then Kong Yong Mohd Hairul Nizam Harun Syed Mohamed Suhail B S Mohamad Angela Ng Min Hwei Chi D Luu Mae-Lynn Catherine Bastion Rescue of photoreceptor with human mesenchyme stem cell and human mesenchyme stem cell expressing erythropoietin in total degeneration of retina animal model Indian Journal of Ophthalmology electroretinography hdumsc hdumsc-epo onl retinal degenerative disease sprague–dawley rat |
| title | Rescue of photoreceptor with human mesenchyme stem cell and human mesenchyme stem cell expressing erythropoietin in total degeneration of retina animal model |
| title_full | Rescue of photoreceptor with human mesenchyme stem cell and human mesenchyme stem cell expressing erythropoietin in total degeneration of retina animal model |
| title_fullStr | Rescue of photoreceptor with human mesenchyme stem cell and human mesenchyme stem cell expressing erythropoietin in total degeneration of retina animal model |
| title_full_unstemmed | Rescue of photoreceptor with human mesenchyme stem cell and human mesenchyme stem cell expressing erythropoietin in total degeneration of retina animal model |
| title_short | Rescue of photoreceptor with human mesenchyme stem cell and human mesenchyme stem cell expressing erythropoietin in total degeneration of retina animal model |
| title_sort | rescue of photoreceptor with human mesenchyme stem cell and human mesenchyme stem cell expressing erythropoietin in total degeneration of retina animal model |
| topic | electroretinography hdumsc hdumsc-epo onl retinal degenerative disease sprague–dawley rat |
| url | http://www.ijo.in/article.asp?issn=0301-4738;year=2022;volume=70;issue=3;spage=921;epage=929;aulast=Abd |
| work_keys_str_mv | AT munirahabdrashid rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT lamchenshen rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT avinkoheehwan rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT hibaalsaeedy rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT pooilingmok rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT sureshkumarsubbiah rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT hazlitamdisa rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT thenkongyong rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT mohdhairulnizamharun rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT syedmohamedsuhailbsmohamad rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT angelangminhwei rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT chidluu rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel AT maelynncatherinebastion rescueofphotoreceptorwithhumanmesenchymestemcellandhumanmesenchymestemcellexpressingerythropoietinintotaldegenerationofretinaanimalmodel |