The anti-photoaging effect of C-phycocyanin on ultraviolet B-irradiated BALB/c-nu mouse skin
Introduction: C-phycocyanin (C-PC), a photosynthetic protein obtained from Spirulina, is regarded a highly promising commercially available biochemical. Numerous in vitro and in vivo studies have provided evidence of C-PC’s ability to mitigate the inflammatory response, alleviate oxidative stress, a...
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| Format: | Article |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1229387/full |
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| author | Yali Zhou Renao Bai Renao Bai Yifeng Huang Weina Li Jiana Chen Zhiyun Cheng Xunxun Wu Yong Diao |
| author_facet | Yali Zhou Renao Bai Renao Bai Yifeng Huang Weina Li Jiana Chen Zhiyun Cheng Xunxun Wu Yong Diao |
| author_sort | Yali Zhou |
| collection | DOAJ |
| description | Introduction: C-phycocyanin (C-PC), a photosynthetic protein obtained from Spirulina, is regarded a highly promising commercially available biochemical. Numerous in vitro and in vivo studies have provided evidence of C-PC’s ability to mitigate the inflammatory response, alleviate oxidative stress, and facilitate wound healing. However, despite the existing knowledge regarding C-PC’s protective mechanism against cellular apoptosis induced by ultraviolet B (UVB) radiation, further in vivo experiments are needed to explore its anti-photoaging mechanism.Methods: In this study, a UVB-induced skin photoaging model was established using BALB/c-nu mice, and the potential protective effects of topically administered c-PC were investigated by various molecular biology tools. In addition, a novel delivery system, C-PC nanodispersion, was developed to facilitate the transdermal delivery of C-PC.Results: C- PC demonstrated significant anti-photoaging activities in the UVB-induced skin. The application of C-PC to the dorsal skin of the mice resulted in improved macroscopic characteristics, such as reduced sagging and coarse wrinkling, under UVB irradiation Histological analyses showed that C-PC treatment significantly decreased the symptoms of epidermal thickening, prevented dermal collagen fiber loosening, increased the hydroxyproline (Hyp) content and activities of antioxidant enzymes (such as superoxide dismutase, catalase, and glutathione peroxidase) in mouse skin, decreased malondialdehyde levels and expressions of inflammatory factors (interleukin-1α [IL-1α], IL-1β, IL-6, and tumor necrosis factor-α), reduced matrix metalloproteinase [MMP-3 and MMP-9] expressions, and inhibited the phosphorylation of c-Jun N-terminal kinase, extracellular signal-regulated kinase, and p38 proteins in the mitogen-activated protein kinase family.Discussion: By analyzing the results of the study, a new drug delivery system, C-PC nano-dispersion, was proposed, and the anti-photoaging effect of C-PC and its mechanism were investigated. |
| first_indexed | 2024-03-12T13:56:33Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-12T13:56:33Z |
| publishDate | 2023-08-01 |
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| spelling | doaj.art-1a60356a8dfe4d109d38925338c892152023-08-22T13:54:00ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-08-011110.3389/fbioe.2023.12293871229387The anti-photoaging effect of C-phycocyanin on ultraviolet B-irradiated BALB/c-nu mouse skinYali Zhou0Renao Bai1Renao Bai2Yifeng Huang3Weina Li4Jiana Chen5Zhiyun Cheng6Xunxun Wu7Yong Diao8School of Biomedical Science, Huaqiao University, Quanzhou, ChinaSchool of Biomedical Science, Huaqiao University, Quanzhou, ChinaShantou Polytechnic, Shantou, ChinaHaixia Hospital Affiliated to Huaqiao University, Quanzhou, ChinaHaixia Hospital Affiliated to Huaqiao University, Quanzhou, ChinaHaixia Hospital Affiliated to Huaqiao University, Quanzhou, ChinaSchool of Biomedical Science, Huaqiao University, Quanzhou, ChinaSchool of Biomedical Science, Huaqiao University, Quanzhou, ChinaSchool of Biomedical Science, Huaqiao University, Quanzhou, ChinaIntroduction: C-phycocyanin (C-PC), a photosynthetic protein obtained from Spirulina, is regarded a highly promising commercially available biochemical. Numerous in vitro and in vivo studies have provided evidence of C-PC’s ability to mitigate the inflammatory response, alleviate oxidative stress, and facilitate wound healing. However, despite the existing knowledge regarding C-PC’s protective mechanism against cellular apoptosis induced by ultraviolet B (UVB) radiation, further in vivo experiments are needed to explore its anti-photoaging mechanism.Methods: In this study, a UVB-induced skin photoaging model was established using BALB/c-nu mice, and the potential protective effects of topically administered c-PC were investigated by various molecular biology tools. In addition, a novel delivery system, C-PC nanodispersion, was developed to facilitate the transdermal delivery of C-PC.Results: C- PC demonstrated significant anti-photoaging activities in the UVB-induced skin. The application of C-PC to the dorsal skin of the mice resulted in improved macroscopic characteristics, such as reduced sagging and coarse wrinkling, under UVB irradiation Histological analyses showed that C-PC treatment significantly decreased the symptoms of epidermal thickening, prevented dermal collagen fiber loosening, increased the hydroxyproline (Hyp) content and activities of antioxidant enzymes (such as superoxide dismutase, catalase, and glutathione peroxidase) in mouse skin, decreased malondialdehyde levels and expressions of inflammatory factors (interleukin-1α [IL-1α], IL-1β, IL-6, and tumor necrosis factor-α), reduced matrix metalloproteinase [MMP-3 and MMP-9] expressions, and inhibited the phosphorylation of c-Jun N-terminal kinase, extracellular signal-regulated kinase, and p38 proteins in the mitogen-activated protein kinase family.Discussion: By analyzing the results of the study, a new drug delivery system, C-PC nano-dispersion, was proposed, and the anti-photoaging effect of C-PC and its mechanism were investigated.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1229387/fullC-phycocyaninskin photoagingultraviolet Bnanodispersionsproinflammatory cytokines |
| spellingShingle | Yali Zhou Renao Bai Renao Bai Yifeng Huang Weina Li Jiana Chen Zhiyun Cheng Xunxun Wu Yong Diao The anti-photoaging effect of C-phycocyanin on ultraviolet B-irradiated BALB/c-nu mouse skin Frontiers in Bioengineering and Biotechnology C-phycocyanin skin photoaging ultraviolet B nanodispersions proinflammatory cytokines |
| title | The anti-photoaging effect of C-phycocyanin on ultraviolet B-irradiated BALB/c-nu mouse skin |
| title_full | The anti-photoaging effect of C-phycocyanin on ultraviolet B-irradiated BALB/c-nu mouse skin |
| title_fullStr | The anti-photoaging effect of C-phycocyanin on ultraviolet B-irradiated BALB/c-nu mouse skin |
| title_full_unstemmed | The anti-photoaging effect of C-phycocyanin on ultraviolet B-irradiated BALB/c-nu mouse skin |
| title_short | The anti-photoaging effect of C-phycocyanin on ultraviolet B-irradiated BALB/c-nu mouse skin |
| title_sort | anti photoaging effect of c phycocyanin on ultraviolet b irradiated balb c nu mouse skin |
| topic | C-phycocyanin skin photoaging ultraviolet B nanodispersions proinflammatory cytokines |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1229387/full |
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