Influence of GSTP1 gene polymorhism on decreased semen quality
Background ― Genetic polymorphisms analysis of metabolic and antioxidant systems pathway genes are associated with male infertility is the most perspective and developed field in andrology. Purpose ― the aim of the research is to reveal the association of the glutathione S-transferase gene GSTP1 po...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Limited liability company «Science and Innovations» (Saratov)
2019-12-01
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| Series: | Russian Open Medical Journal |
| Subjects: | |
| Online Access: | http://www.romj.org/node/287 |
| _version_ | 1830274040214847488 |
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| author | Galina I. Myandina Alhejoj Hasan Madina M. Azova Ekaterina V. Tarasenko Nina G. Kulchenko |
| author_facet | Galina I. Myandina Alhejoj Hasan Madina M. Azova Ekaterina V. Tarasenko Nina G. Kulchenko |
| author_sort | Galina I. Myandina |
| collection | DOAJ |
| description | Background ― Genetic polymorphisms analysis of metabolic and antioxidant systems pathway genes are associated with male infertility is the most perspective and developed field in andrology.
Purpose ― the aim of the research is to reveal the association of the glutathione S-transferase gene GSTP1 polymorphism C/T (rs1138272) with risk of pathospermia in Russian men in Moscow region.
Material and Methods ― Case control study was conducted on 68 fertile men and 70 infertile men with various forms of pathospermia. Sperm analysis was performed according to WHO guidelines (WHO, 2010). DNA was extracted from peripheral blood leukocytes. Genotyping of the GSTP1 gene polymorphisms was carried out by generated amplicons from melting curve analysis after real time PCR.
Results ― statistically significant association of polymorphism GSTP1 C/T (Ala/Val; rs1138272) with asthenozoospermia (χ2=8.58, p=0.003) and teratospermia (χ2=6.81, p=0.009) risk was found. The frequencies of homozygous and heterozygous carries (CT+TT genotypes) for polymorphic locus GSTP1 gene (rs1138272) are 3 times higher for men with disturbance of motility of spermatozoa and 2.5 times higher for men with abnormalities in morphology of spermatozoa, then for men with normozoospermia.
Conclusion ― The GSTP1 C>T polymorphism (rs1138272) associated with risk of teratospermia and asthenozoospermia in male of reproductive age. |
| first_indexed | 2024-12-18T23:55:35Z |
| format | Article |
| id | doaj.art-1a60c59b4002455ea78ffc7a8ae49eb1 |
| institution | Directory Open Access Journal |
| issn | 2304-3415 |
| language | English |
| last_indexed | 2024-12-18T23:55:35Z |
| publishDate | 2019-12-01 |
| publisher | Limited liability company «Science and Innovations» (Saratov) |
| record_format | Article |
| series | Russian Open Medical Journal |
| spelling | doaj.art-1a60c59b4002455ea78ffc7a8ae49eb12022-12-21T20:46:42ZengLimited liability company «Science and Innovations» (Saratov)Russian Open Medical Journal2304-34152019-12-0184e041110.15275/rusomj.2019.0411Influence of GSTP1 gene polymorhism on decreased semen qualityGalina I. MyandinaAlhejoj HasanMadina M. AzovaEkaterina V. TarasenkoNina G. KulchenkoBackground ― Genetic polymorphisms analysis of metabolic and antioxidant systems pathway genes are associated with male infertility is the most perspective and developed field in andrology. Purpose ― the aim of the research is to reveal the association of the glutathione S-transferase gene GSTP1 polymorphism C/T (rs1138272) with risk of pathospermia in Russian men in Moscow region. Material and Methods ― Case control study was conducted on 68 fertile men and 70 infertile men with various forms of pathospermia. Sperm analysis was performed according to WHO guidelines (WHO, 2010). DNA was extracted from peripheral blood leukocytes. Genotyping of the GSTP1 gene polymorphisms was carried out by generated amplicons from melting curve analysis after real time PCR. Results ― statistically significant association of polymorphism GSTP1 C/T (Ala/Val; rs1138272) with asthenozoospermia (χ2=8.58, p=0.003) and teratospermia (χ2=6.81, p=0.009) risk was found. The frequencies of homozygous and heterozygous carries (CT+TT genotypes) for polymorphic locus GSTP1 gene (rs1138272) are 3 times higher for men with disturbance of motility of spermatozoa and 2.5 times higher for men with abnormalities in morphology of spermatozoa, then for men with normozoospermia. Conclusion ― The GSTP1 C>T polymorphism (rs1138272) associated with risk of teratospermia and asthenozoospermia in male of reproductive age.http://www.romj.org/node/287oxidative stressmale infertilitygstp1 genepolymorphismpathospermiaasthenozoospermiateratospermia |
| spellingShingle | Galina I. Myandina Alhejoj Hasan Madina M. Azova Ekaterina V. Tarasenko Nina G. Kulchenko Influence of GSTP1 gene polymorhism on decreased semen quality Russian Open Medical Journal oxidative stress male infertility gstp1 gene polymorphism pathospermia asthenozoospermia teratospermia |
| title | Influence of GSTP1 gene polymorhism on decreased semen quality |
| title_full | Influence of GSTP1 gene polymorhism on decreased semen quality |
| title_fullStr | Influence of GSTP1 gene polymorhism on decreased semen quality |
| title_full_unstemmed | Influence of GSTP1 gene polymorhism on decreased semen quality |
| title_short | Influence of GSTP1 gene polymorhism on decreased semen quality |
| title_sort | influence of gstp1 gene polymorhism on decreased semen quality |
| topic | oxidative stress male infertility gstp1 gene polymorphism pathospermia asthenozoospermia teratospermia |
| url | http://www.romj.org/node/287 |
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