Association of Platelet Desialylation and Circulating Follicular Helper T Cells in Patients With Thrombocytopenia
Thrombocytopenia is a multifactorial condition that frequently involves concomitant defects in platelet production and clearance. The physiopathology of low platelet count in thrombocytopenia remains unclear. Sialylation on platelet membrane glycoprotein and follicular helper T cells (TFHs) are thou...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.810620/full |
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| author | Yuwen Chen Liping Luo Yongzhi Zheng Qiaoyun Zheng Na Zhang Donghui Gan Shimuye Kalayu Yirga Zhenxing Lin Qizhen Shi Qizhen Shi Lin Fu Jianda Hu Yingyu Chen |
| author_facet | Yuwen Chen Liping Luo Yongzhi Zheng Qiaoyun Zheng Na Zhang Donghui Gan Shimuye Kalayu Yirga Zhenxing Lin Qizhen Shi Qizhen Shi Lin Fu Jianda Hu Yingyu Chen |
| author_sort | Yuwen Chen |
| collection | DOAJ |
| description | Thrombocytopenia is a multifactorial condition that frequently involves concomitant defects in platelet production and clearance. The physiopathology of low platelet count in thrombocytopenia remains unclear. Sialylation on platelet membrane glycoprotein and follicular helper T cells (TFHs) are thought to be the novel platelet clearance pathways. The aim of this study was to clarify the roles of platelet desialylation and circulating TFHs in patients with immune thrombocytopenia (ITP) and non-ITP thrombocytopenia. We enrolled 190 patients with ITP and 94 patients with non-ITP related thrombocytopenia including case of aplastic anemia (AA) and myelodysplastic syndromes (MDS). One hundred and ten healthy volunteers were included as controls. We found significantly increased desialylated platelets in patients with ITP or thrombocytopenia in the context of AA and MDS. Platelet desialylation was negatively correlated with platelet count. Meanwhile, the circulating TFH levels in patients with thrombocytopenia were significantly higher than those of normal controls, and were positively correlated with desialylated platelet levels. Moreover, TFHs-related chemokine CXCL13 and apoptotic platelet levels were abnormally high in ITP patients. The upregulation of pro-apoptotic proteins and the activation of the MAPK/mTOR pathway were observed in the same cohort. These findings suggested that platelet desialylation and circulating TFHs may become the potential biomarkers for evaluating the disease process associated with thrombocytopenia in patients with ITP and non-ITP. |
| first_indexed | 2024-12-22T23:45:06Z |
| format | Article |
| id | doaj.art-1a632389f5584df0b5f37d8d1862a200 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-22T23:45:06Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-1a632389f5584df0b5f37d8d1862a2002022-12-21T18:08:17ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-04-011310.3389/fimmu.2022.810620810620Association of Platelet Desialylation and Circulating Follicular Helper T Cells in Patients With ThrombocytopeniaYuwen Chen0Liping Luo1Yongzhi Zheng2Qiaoyun Zheng3Na Zhang4Donghui Gan5Shimuye Kalayu Yirga6Zhenxing Lin7Qizhen Shi8Qizhen Shi9Lin Fu10Jianda Hu11Yingyu Chen12Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Pediatrics, Medical College of Wisconsin, Milwaukee, WI, United StatesBlood Research Institute, Versiti, Milwaukee, WI, United StatesDepartment of Hematology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, ChinaThrombocytopenia is a multifactorial condition that frequently involves concomitant defects in platelet production and clearance. The physiopathology of low platelet count in thrombocytopenia remains unclear. Sialylation on platelet membrane glycoprotein and follicular helper T cells (TFHs) are thought to be the novel platelet clearance pathways. The aim of this study was to clarify the roles of platelet desialylation and circulating TFHs in patients with immune thrombocytopenia (ITP) and non-ITP thrombocytopenia. We enrolled 190 patients with ITP and 94 patients with non-ITP related thrombocytopenia including case of aplastic anemia (AA) and myelodysplastic syndromes (MDS). One hundred and ten healthy volunteers were included as controls. We found significantly increased desialylated platelets in patients with ITP or thrombocytopenia in the context of AA and MDS. Platelet desialylation was negatively correlated with platelet count. Meanwhile, the circulating TFH levels in patients with thrombocytopenia were significantly higher than those of normal controls, and were positively correlated with desialylated platelet levels. Moreover, TFHs-related chemokine CXCL13 and apoptotic platelet levels were abnormally high in ITP patients. The upregulation of pro-apoptotic proteins and the activation of the MAPK/mTOR pathway were observed in the same cohort. These findings suggested that platelet desialylation and circulating TFHs may become the potential biomarkers for evaluating the disease process associated with thrombocytopenia in patients with ITP and non-ITP.https://www.frontiersin.org/articles/10.3389/fimmu.2022.810620/fullthrombocytopeniaplateletdesialylationfollicular helper T Cells (TFHs)CXCL13 |
| spellingShingle | Yuwen Chen Liping Luo Yongzhi Zheng Qiaoyun Zheng Na Zhang Donghui Gan Shimuye Kalayu Yirga Zhenxing Lin Qizhen Shi Qizhen Shi Lin Fu Jianda Hu Yingyu Chen Association of Platelet Desialylation and Circulating Follicular Helper T Cells in Patients With Thrombocytopenia Frontiers in Immunology thrombocytopenia platelet desialylation follicular helper T Cells (TFHs) CXCL13 |
| title | Association of Platelet Desialylation and Circulating Follicular Helper T Cells in Patients With Thrombocytopenia |
| title_full | Association of Platelet Desialylation and Circulating Follicular Helper T Cells in Patients With Thrombocytopenia |
| title_fullStr | Association of Platelet Desialylation and Circulating Follicular Helper T Cells in Patients With Thrombocytopenia |
| title_full_unstemmed | Association of Platelet Desialylation and Circulating Follicular Helper T Cells in Patients With Thrombocytopenia |
| title_short | Association of Platelet Desialylation and Circulating Follicular Helper T Cells in Patients With Thrombocytopenia |
| title_sort | association of platelet desialylation and circulating follicular helper t cells in patients with thrombocytopenia |
| topic | thrombocytopenia platelet desialylation follicular helper T Cells (TFHs) CXCL13 |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.810620/full |
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