The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures
Introduction The present study aimed to investigate the potential effects of rivaroxaban, an oral anticoagulant that inhibits the effects of factor Xa, on intact intervertebral disc tissue cells and the extracellular matrix (ECM). Material and methods Rivaroxaban was applied to primary human cell c...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2021-05-01
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| Series: | Archives of Medical Science |
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| Online Access: | https://www.archivesofmedicalscience.com/The-effects-of-rivaroxaban-an-oral-anticoagulant-on-human-IVD-primary-cultures,136323,0,2.html |
| _version_ | 1797435297781252096 |
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| author | Tezcan Caliskan Hande Akalan Ibrahim Yilmaz Numan Karaarslan Duygu Yasar Sirin Hanefi Ozbek |
| author_facet | Tezcan Caliskan Hande Akalan Ibrahim Yilmaz Numan Karaarslan Duygu Yasar Sirin Hanefi Ozbek |
| author_sort | Tezcan Caliskan |
| collection | DOAJ |
| description | Introduction
The present study aimed to investigate the potential effects
of rivaroxaban, an oral anticoagulant that inhibits the effects of factor Xa,
on intact intervertebral disc tissue cells and the extracellular matrix (ECM).
Material and methods
Rivaroxaban was applied to primary human cell cultures prepared from tissues of the intervertebral disc. Comparative molecular analyses were performed on non-drug-treated control group samples.
Descriptive statistics were presented as the mean ± standard deviation. An
analysis of variance test was performed to determine whether there were significant differences in the mean across the groups. When differences across
groups were observed, Tukey’s honestly significant difference post-hoc test
was used for multiple pairwise comparisons. The significance of the obtained
data was determined statistically. The α significance value was < 0.05.
Results
The cells in the control group and in the rivaroxaban-treated group
were viable, healthy, and proliferated (p < 0.05). However, the expression
levels of the chondroadherin gene (CHAD), cartilage oligo matrix protein
(COMP), matrix metalloproteinase (MMP)-13, and MMP-19 genes were
changed (p < 0.05).
Conclusions
Although rivaroxaban does not suppress cell proliferation due
to morphological, biological, and biochemical changes in the intervertebral
disc tissue, it may change the expression of genes that are related to ECM
maintenance. |
| first_indexed | 2024-03-09T10:46:09Z |
| format | Article |
| id | doaj.art-1a6a7dbc370a4314b5c8ce12521276b1 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-03-09T10:46:09Z |
| publishDate | 2021-05-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-1a6a7dbc370a4314b5c8ce12521276b12023-12-01T10:02:33ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512021-05-011841062107010.5114/aoms/136323136323The effects of rivaroxaban, an oral anticoagulant, on human IVD primary culturesTezcan Caliskan0https://orcid.org/0000-0001-7735-0584Hande Akalan1https://orcid.org/0000-0002-5922-2498Ibrahim Yilmaz2https://orcid.org/0000-0003-2003-6337Numan Karaarslan3https://orcid.org/0000-0001-5590-0637Duygu Yasar Sirin4https://orcid.org/0000-0002-1224-442XHanefi Ozbek5https://orcid.org/0000-0002-8084-7855Department of Neurosurgery, School of Medicine, Namik Kemal University, Tekirdag, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Arts and Sciences, Namik Kemal University, Tekirdag, TurkeyDepartment of Medical Pharmacology, School of Medicine, Istanbul Medipol University, Istanbul, TurkeyDepartment of Neurosurgery, School of Medicine, Halic University, Istanbul, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Arts and Sciences, Namik Kemal University, Tekirdag, TurkeyDepartment of Medical Pharmacology, Bakircay University School of Medicine, Izmir, TurkeyIntroduction The present study aimed to investigate the potential effects of rivaroxaban, an oral anticoagulant that inhibits the effects of factor Xa, on intact intervertebral disc tissue cells and the extracellular matrix (ECM). Material and methods Rivaroxaban was applied to primary human cell cultures prepared from tissues of the intervertebral disc. Comparative molecular analyses were performed on non-drug-treated control group samples. Descriptive statistics were presented as the mean ± standard deviation. An analysis of variance test was performed to determine whether there were significant differences in the mean across the groups. When differences across groups were observed, Tukey’s honestly significant difference post-hoc test was used for multiple pairwise comparisons. The significance of the obtained data was determined statistically. The α significance value was < 0.05. Results The cells in the control group and in the rivaroxaban-treated group were viable, healthy, and proliferated (p < 0.05). However, the expression levels of the chondroadherin gene (CHAD), cartilage oligo matrix protein (COMP), matrix metalloproteinase (MMP)-13, and MMP-19 genes were changed (p < 0.05). Conclusions Although rivaroxaban does not suppress cell proliferation due to morphological, biological, and biochemical changes in the intervertebral disc tissue, it may change the expression of genes that are related to ECM maintenance.https://www.archivesofmedicalscience.com/The-effects-of-rivaroxaban-an-oral-anticoagulant-on-human-IVD-primary-cultures,136323,0,2.htmlcartilage oligo matrix proteinchondroadherin genecytotoxicityintervertebral disc cellsmatrix metalloproteinasesnucleus pulposusrivaroxaban |
| spellingShingle | Tezcan Caliskan Hande Akalan Ibrahim Yilmaz Numan Karaarslan Duygu Yasar Sirin Hanefi Ozbek The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures Archives of Medical Science cartilage oligo matrix protein chondroadherin gene cytotoxicity intervertebral disc cells matrix metalloproteinases nucleus pulposus rivaroxaban |
| title | The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures |
| title_full | The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures |
| title_fullStr | The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures |
| title_full_unstemmed | The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures |
| title_short | The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures |
| title_sort | effects of rivaroxaban an oral anticoagulant on human ivd primary cultures |
| topic | cartilage oligo matrix protein chondroadherin gene cytotoxicity intervertebral disc cells matrix metalloproteinases nucleus pulposus rivaroxaban |
| url | https://www.archivesofmedicalscience.com/The-effects-of-rivaroxaban-an-oral-anticoagulant-on-human-IVD-primary-cultures,136323,0,2.html |
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