Engineered Liposomes Protect Immortalized Immune Cells from Cytolysins Secreted by Group A and Group G Streptococci
The increasing antibiotic resistance of bacterial pathogens fosters the development of alternative, non-antibiotic treatments. Antivirulence therapy, which is neither bacteriostatic nor bactericidal, acts by depriving bacterial pathogens of their virulence factors. To establish a successful infectio...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/1/166 |
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| author | Hervé Besançon Yu Larpin Viktoria S. Babiychuk René Köffel Eduard B. Babiychuk |
| author_facet | Hervé Besançon Yu Larpin Viktoria S. Babiychuk René Köffel Eduard B. Babiychuk |
| author_sort | Hervé Besançon |
| collection | DOAJ |
| description | The increasing antibiotic resistance of bacterial pathogens fosters the development of alternative, non-antibiotic treatments. Antivirulence therapy, which is neither bacteriostatic nor bactericidal, acts by depriving bacterial pathogens of their virulence factors. To establish a successful infection, many bacterial pathogens secrete exotoxins/cytolysins that perforate the host cell plasma membrane. Recently developed liposomal nanotraps, mimicking the outer layer of the targeted cell membranes, serve as decoys for exotoxins, thus diverting them from attacking host cells. In this study, we develop a liposomal nanotrap formulation that is capable of protecting immortalized immune cells from the whole palette of cytolysins secreted by <i>Streptococcus pyogenes</i> and <i>Streptococcus dysgalactiae</i> subsp. <i>equisimilis</i>—important human pathogens that can cause life-threatening bacteremia. We show that the mixture of cholesterol-containing liposomes with liposomes composed exclusively of phospholipids is protective against the combined action of all streptococcal exotoxins. Our findings pave the way for further development of liposomal antivirulence therapy in order to provide more efficient treatment of bacterial infections, including those caused by antibiotic resistant pathogens. |
| first_indexed | 2024-03-10T03:45:07Z |
| format | Article |
| id | doaj.art-1a6c0cf01d9f4eb68bc7fbae3a765c70 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T03:45:07Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-1a6c0cf01d9f4eb68bc7fbae3a765c702023-11-23T11:21:11ZengMDPI AGCells2073-44092022-01-0111116610.3390/cells11010166Engineered Liposomes Protect Immortalized Immune Cells from Cytolysins Secreted by Group A and Group G StreptococciHervé Besançon0Yu Larpin1Viktoria S. Babiychuk2René Köffel3Eduard B. Babiychuk4Institute of Anatomy, Faculty of Medicine, University of Bern, 3012 Bern, SwitzerlandInstitute of Anatomy, Faculty of Medicine, University of Bern, 3012 Bern, SwitzerlandInstitute of Anatomy, Faculty of Medicine, University of Bern, 3012 Bern, SwitzerlandInstitute of Anatomy, Faculty of Medicine, University of Bern, 3012 Bern, SwitzerlandInstitute of Anatomy, Faculty of Medicine, University of Bern, 3012 Bern, SwitzerlandThe increasing antibiotic resistance of bacterial pathogens fosters the development of alternative, non-antibiotic treatments. Antivirulence therapy, which is neither bacteriostatic nor bactericidal, acts by depriving bacterial pathogens of their virulence factors. To establish a successful infection, many bacterial pathogens secrete exotoxins/cytolysins that perforate the host cell plasma membrane. Recently developed liposomal nanotraps, mimicking the outer layer of the targeted cell membranes, serve as decoys for exotoxins, thus diverting them from attacking host cells. In this study, we develop a liposomal nanotrap formulation that is capable of protecting immortalized immune cells from the whole palette of cytolysins secreted by <i>Streptococcus pyogenes</i> and <i>Streptococcus dysgalactiae</i> subsp. <i>equisimilis</i>—important human pathogens that can cause life-threatening bacteremia. We show that the mixture of cholesterol-containing liposomes with liposomes composed exclusively of phospholipids is protective against the combined action of all streptococcal exotoxins. Our findings pave the way for further development of liposomal antivirulence therapy in order to provide more efficient treatment of bacterial infections, including those caused by antibiotic resistant pathogens.https://www.mdpi.com/2073-4409/11/1/166antibiotic resistancebacterial infection<i>Streptococcus</i>toxinliposomenanotrap |
| spellingShingle | Hervé Besançon Yu Larpin Viktoria S. Babiychuk René Köffel Eduard B. Babiychuk Engineered Liposomes Protect Immortalized Immune Cells from Cytolysins Secreted by Group A and Group G Streptococci Cells antibiotic resistance bacterial infection <i>Streptococcus</i> toxin liposome nanotrap |
| title | Engineered Liposomes Protect Immortalized Immune Cells from Cytolysins Secreted by Group A and Group G Streptococci |
| title_full | Engineered Liposomes Protect Immortalized Immune Cells from Cytolysins Secreted by Group A and Group G Streptococci |
| title_fullStr | Engineered Liposomes Protect Immortalized Immune Cells from Cytolysins Secreted by Group A and Group G Streptococci |
| title_full_unstemmed | Engineered Liposomes Protect Immortalized Immune Cells from Cytolysins Secreted by Group A and Group G Streptococci |
| title_short | Engineered Liposomes Protect Immortalized Immune Cells from Cytolysins Secreted by Group A and Group G Streptococci |
| title_sort | engineered liposomes protect immortalized immune cells from cytolysins secreted by group a and group g streptococci |
| topic | antibiotic resistance bacterial infection <i>Streptococcus</i> toxin liposome nanotrap |
| url | https://www.mdpi.com/2073-4409/11/1/166 |
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