Reusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids
When developing a biocatalyzed synthesis route, the enzyme reusability is an important parameter to consider for the reduction of industrial costs. In this context, the functional stability of Novozym® 435 in ionic liquids (ILs) was studied in the transesterification of mannose with vinyl myristate....
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| Format: | Article |
| Language: | English |
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Presses Agronomiques de Gembloux
2013-01-01
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| Series: | Biotechnologie, Agronomie, Société et Environnement |
| Subjects: | |
| Online Access: | http://hdl.handle.net/11006/112 |
| _version_ | 1818268761104318464 |
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| author | Galonde, N. Richard, G. Deleu, M. Nott, K. Jerôme, C. Fauconnier, ML. |
| author_facet | Galonde, N. Richard, G. Deleu, M. Nott, K. Jerôme, C. Fauconnier, ML. |
| author_sort | Galonde, N. |
| collection | DOAJ |
| description | When developing a biocatalyzed synthesis route, the enzyme reusability is an important parameter to consider for the reduction of industrial costs. In this context, the functional stability of Novozym® 435 in ionic liquids (ILs) was studied in the transesterification of mannose with vinyl myristate. The enzyme was re-used five times in three ILs, 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim][BF4]), 1-butyl-3-methylimidazolium trifluoromethanesulfonate ([Bmim][TFO]), 1-butyl-1-methylpyrrolidinium trifluoromethanesulfonate ([Bmpyrr][TFO]) and in tert-butanol (tert-BuOH). [Bmpyrr][TFO] showed the best 24 h-yield (24 h-η), with 68.8% after the first cycle and the lowest loss of 24 h-η (42%) after five cycles (24 h-η of 39.9%). In comparison with [Bmpyrr][TFO], Novozym® 435 presented the most prominent loss of activity after five cycles of reaction in [Bmim][TFO] (loss of 89%), despite the good 24 h-η obtained after one cycle (60%). [Bmim][BF4] was the least interesting IL, as it was found to lead to the lowest 24 h-η, with 24.5% after one cycle and a significant loss of activity (77%) after five cycles, with a 24 h-η of 5.6%. After five cycles, the 24 h-η in [Bmpyrr][TFO] was higher than in tert-BuOH and the yield loss was higher for the organic solvent (57%). Consequently, these results reveal that, in the present study, the pyrrolidium-based IL [Bmpyrr][TFO] represented the best IL as it allowed the highest level of enzymatic activity and functional stability of Novozym® 435. |
| first_indexed | 2024-12-12T20:43:37Z |
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| id | doaj.art-1a6f3d3af13d46679bbfe268000ea272 |
| institution | Directory Open Access Journal |
| issn | 1370-6233 1780-4507 |
| language | English |
| last_indexed | 2024-12-12T20:43:37Z |
| publishDate | 2013-01-01 |
| publisher | Presses Agronomiques de Gembloux |
| record_format | Article |
| series | Biotechnologie, Agronomie, Société et Environnement |
| spelling | doaj.art-1a6f3d3af13d46679bbfe268000ea2722022-12-22T00:12:38ZengPresses Agronomiques de GemblouxBiotechnologie, Agronomie, Société et Environnement1370-62331780-45072013-01-01174556562Reusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquidsGalonde, N.Richard, G.Deleu, M.Nott, K.Jerôme, C.Fauconnier, ML.When developing a biocatalyzed synthesis route, the enzyme reusability is an important parameter to consider for the reduction of industrial costs. In this context, the functional stability of Novozym® 435 in ionic liquids (ILs) was studied in the transesterification of mannose with vinyl myristate. The enzyme was re-used five times in three ILs, 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim][BF4]), 1-butyl-3-methylimidazolium trifluoromethanesulfonate ([Bmim][TFO]), 1-butyl-1-methylpyrrolidinium trifluoromethanesulfonate ([Bmpyrr][TFO]) and in tert-butanol (tert-BuOH). [Bmpyrr][TFO] showed the best 24 h-yield (24 h-η), with 68.8% after the first cycle and the lowest loss of 24 h-η (42%) after five cycles (24 h-η of 39.9%). In comparison with [Bmpyrr][TFO], Novozym® 435 presented the most prominent loss of activity after five cycles of reaction in [Bmim][TFO] (loss of 89%), despite the good 24 h-η obtained after one cycle (60%). [Bmim][BF4] was the least interesting IL, as it was found to lead to the lowest 24 h-η, with 24.5% after one cycle and a significant loss of activity (77%) after five cycles, with a 24 h-η of 5.6%. After five cycles, the 24 h-η in [Bmpyrr][TFO] was higher than in tert-BuOH and the yield loss was higher for the organic solvent (57%). Consequently, these results reveal that, in the present study, the pyrrolidium-based IL [Bmpyrr][TFO] represented the best IL as it allowed the highest level of enzymatic activity and functional stability of Novozym® 435.http://hdl.handle.net/11006/112Esterscarbohydratesesterificationlipasesenzyme technology |
| spellingShingle | Galonde, N. Richard, G. Deleu, M. Nott, K. Jerôme, C. Fauconnier, ML. Reusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids Biotechnologie, Agronomie, Société et Environnement Esters carbohydrates esterification lipases enzyme technology |
| title | Reusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids |
| title_full | Reusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids |
| title_fullStr | Reusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids |
| title_full_unstemmed | Reusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids |
| title_short | Reusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids |
| title_sort | reusability study of novozym r 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids |
| topic | Esters carbohydrates esterification lipases enzyme technology |
| url | http://hdl.handle.net/11006/112 |
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