Thermodynamic analysis of enzymatic enantioselectivity of lipase-catalyzed hydrolysis of ibuprofen vinyl ester by different solvent medium(溶剂调控布洛芬乙烯酯酶促水解的立体选择性的热力学研究)
采用高效液相色谱研究了不同溶剂体系中褶皱假丝酵母菌脂肪酶(CRL)催化布洛芬乙烯酯水解反应的立体选择性.研究发现，在单一溶剂体系中Eyring曲线呈线性，在10~50 ℃的实验温度内均呈S选择性；在混合溶剂体系中Eyring曲线呈非线性，且观察到不同的转变温度(Tinv).研究还发现：在亲水性有机溶剂二氧六环/水均相混合溶剂体系中，Eyring曲线的Tinv处立体选择性E值为极小值，在疏水性有机溶剂异辛烷/水两相混合溶剂体系中，Tinv处E值为极大值，而在氯仿/水两相混合溶剂体系中，随着温度的变化，立体选择性发生了反转....
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2013-09-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2013.05.016 |
| _version_ | 1797235878410584064 |
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| author | WANGTing-ting(王婷婷) JINXin(金鑫) WUQi(吴起) LINXian-fu(林贤福) |
| author_facet | WANGTing-ting(王婷婷) JINXin(金鑫) WUQi(吴起) LINXian-fu(林贤福) |
| author_sort | WANGTing-ting(王婷婷) |
| collection | DOAJ |
| description | 采用高效液相色谱研究了不同溶剂体系中褶皱假丝酵母菌脂肪酶(CRL)催化布洛芬乙烯酯水解反应的立体选择性.研究发现，在单一溶剂体系中Eyring曲线呈线性，在10~50 ℃的实验温度内均呈S选择性；在混合溶剂体系中Eyring曲线呈非线性，且观察到不同的转变温度(Tinv).研究还发现：在亲水性有机溶剂二氧六环/水均相混合溶剂体系中，Eyring曲线的Tinv处立体选择性E值为极小值，在疏水性有机溶剂异辛烷/水两相混合溶剂体系中，Tinv处E值为极大值，而在氯仿/水两相混合溶剂体系中，随着温度的变化，立体选择性发生了反转. |
| first_indexed | 2024-04-24T16:54:57Z |
| format | Article |
| id | doaj.art-1a71e2a4f5074f9094011b1d9d9c9127 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T16:54:57Z |
| publishDate | 2013-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-1a71e2a4f5074f9094011b1d9d9c91272024-03-29T01:58:32ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972013-09-0140555355810.3785/j.issn.1008-9497.2013.05.016Thermodynamic analysis of enzymatic enantioselectivity of lipase-catalyzed hydrolysis of ibuprofen vinyl ester by different solvent medium(溶剂调控布洛芬乙烯酯酶促水解的立体选择性的热力学研究)WANGTing-ting(王婷婷)0JINXin(金鑫)1WUQi(吴起)2LINXian-fu(林贤福)3Department of Chemistry, Zhejiang University, Hangzhou 310027, China(浙江大学 化学系，浙江 杭州 310027)Department of Chemistry, Zhejiang University, Hangzhou 310027, China(浙江大学 化学系，浙江 杭州 310027)Department of Chemistry, Zhejiang University, Hangzhou 310027, China(浙江大学 化学系，浙江 杭州 310027)Department of Chemistry, Zhejiang University, Hangzhou 310027, China(浙江大学 化学系，浙江 杭州 310027)采用高效液相色谱研究了不同溶剂体系中褶皱假丝酵母菌脂肪酶(CRL)催化布洛芬乙烯酯水解反应的立体选择性.研究发现，在单一溶剂体系中Eyring曲线呈线性，在10~50 ℃的实验温度内均呈S选择性；在混合溶剂体系中Eyring曲线呈非线性，且观察到不同的转变温度(Tinv).研究还发现：在亲水性有机溶剂二氧六环/水均相混合溶剂体系中，Eyring曲线的Tinv处立体选择性E值为极小值，在疏水性有机溶剂异辛烷/水两相混合溶剂体系中，Tinv处E值为极大值，而在氯仿/水两相混合溶剂体系中，随着温度的变化，立体选择性发生了反转.https://doi.org/10.3785/j.issn.1008-9497.2013.05.016布洛芬乙烯酯酶促立体选择性反应介质转变温度褶皱假丝酵母菌脂肪酶 |
| spellingShingle | WANGTing-ting(王婷婷) JINXin(金鑫) WUQi(吴起) LINXian-fu(林贤福) Thermodynamic analysis of enzymatic enantioselectivity of lipase-catalyzed hydrolysis of ibuprofen vinyl ester by different solvent medium(溶剂调控布洛芬乙烯酯酶促水解的立体选择性的热力学研究) Zhejiang Daxue xuebao. Lixue ban 布洛芬乙烯酯 酶促立体选择性 反应介质 转变温度 褶皱假丝酵母菌脂肪酶 |
| title | Thermodynamic analysis of enzymatic enantioselectivity of lipase-catalyzed hydrolysis of ibuprofen vinyl ester by different solvent medium(溶剂调控布洛芬乙烯酯酶促水解的立体选择性的热力学研究) |
| title_full | Thermodynamic analysis of enzymatic enantioselectivity of lipase-catalyzed hydrolysis of ibuprofen vinyl ester by different solvent medium(溶剂调控布洛芬乙烯酯酶促水解的立体选择性的热力学研究) |
| title_fullStr | Thermodynamic analysis of enzymatic enantioselectivity of lipase-catalyzed hydrolysis of ibuprofen vinyl ester by different solvent medium(溶剂调控布洛芬乙烯酯酶促水解的立体选择性的热力学研究) |
| title_full_unstemmed | Thermodynamic analysis of enzymatic enantioselectivity of lipase-catalyzed hydrolysis of ibuprofen vinyl ester by different solvent medium(溶剂调控布洛芬乙烯酯酶促水解的立体选择性的热力学研究) |
| title_short | Thermodynamic analysis of enzymatic enantioselectivity of lipase-catalyzed hydrolysis of ibuprofen vinyl ester by different solvent medium(溶剂调控布洛芬乙烯酯酶促水解的立体选择性的热力学研究) |
| title_sort | thermodynamic analysis of enzymatic enantioselectivity of lipase catalyzed hydrolysis of ibuprofen vinyl ester by different solvent medium 溶剂调控布洛芬乙烯酯酶促水解的立体选择性的热力学研究 |
| topic | 布洛芬乙烯酯 酶促立体选择性 反应介质 转变温度 褶皱假丝酵母菌脂肪酶 |
| url | https://doi.org/10.3785/j.issn.1008-9497.2013.05.016 |
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