A pilot study of microRNA assessment as a means to identify novel biomarkers of spontaneous osteoarthritis in dogs
Abstract MicroRNAs (miRNAs) are important regulators of intercellular signaling and are promising biomarkers in osteoarthritis (OA). In this study, comprehensive analysis was performed to identify miRNAs involved in the pathogenesis of spontaneous OA in dogs. Dogs diagnosed with OA based on radiogra...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-22362-2 |
| _version_ | 1811257054608752640 |
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| author | Atsushi Yamazaki Yuma Tomo Hinano Eto Koji Tanegashima Kazuya Edamura |
| author_facet | Atsushi Yamazaki Yuma Tomo Hinano Eto Koji Tanegashima Kazuya Edamura |
| author_sort | Atsushi Yamazaki |
| collection | DOAJ |
| description | Abstract MicroRNAs (miRNAs) are important regulators of intercellular signaling and are promising biomarkers in osteoarthritis (OA). In this study, comprehensive analysis was performed to identify miRNAs involved in the pathogenesis of spontaneous OA in dogs. Dogs diagnosed with OA based on radiography and arthroscopy of the stifle joint were included in the OA group. Dogs without any evidence of orthopedic disease were included in the unaffected group. To investigate miRNA expression levels, RNA sequencing analysis (RNA-seq) was performed in synovial tissue (OA group: n = 3, Unaffected group: n = 3) and RT-qPCR was performed in synovial tissue, synovial fluid and serum (OA group: n = 17, Unaffected group: n = 6), and compared between the two groups. The RNA-seq results showed that 57 miRNAs were significantly upregulated and 42 were significantly downregulated in the OA group. Specifically, miR-542 and miR-543 expression levels in the synovial tissue, synovial fluid, and serum were consistently higher in the OA group than in the unaffected group, suggesting that these miRNAs may be used as biomarkers for detecting canine OA. This is the first report to comprehensively analyze the expression patterns of miRNAs in the synovial tissue of dogs with spontaneous OA. |
| first_indexed | 2024-04-12T17:50:34Z |
| format | Article |
| id | doaj.art-1a73601f7d574c0581d3c1ec1567a758 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T17:50:34Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1a73601f7d574c0581d3c1ec1567a7582022-12-22T03:22:30ZengNature PortfolioScientific Reports2045-23222022-10-0112111110.1038/s41598-022-22362-2A pilot study of microRNA assessment as a means to identify novel biomarkers of spontaneous osteoarthritis in dogsAtsushi Yamazaki0Yuma Tomo1Hinano Eto2Koji Tanegashima3Kazuya Edamura4Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource and Sciences, Nihon UniversityLaboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource and Sciences, Nihon UniversityLaboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource and Sciences, Nihon UniversityLaboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource and Sciences, Nihon UniversityLaboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource and Sciences, Nihon UniversityAbstract MicroRNAs (miRNAs) are important regulators of intercellular signaling and are promising biomarkers in osteoarthritis (OA). In this study, comprehensive analysis was performed to identify miRNAs involved in the pathogenesis of spontaneous OA in dogs. Dogs diagnosed with OA based on radiography and arthroscopy of the stifle joint were included in the OA group. Dogs without any evidence of orthopedic disease were included in the unaffected group. To investigate miRNA expression levels, RNA sequencing analysis (RNA-seq) was performed in synovial tissue (OA group: n = 3, Unaffected group: n = 3) and RT-qPCR was performed in synovial tissue, synovial fluid and serum (OA group: n = 17, Unaffected group: n = 6), and compared between the two groups. The RNA-seq results showed that 57 miRNAs were significantly upregulated and 42 were significantly downregulated in the OA group. Specifically, miR-542 and miR-543 expression levels in the synovial tissue, synovial fluid, and serum were consistently higher in the OA group than in the unaffected group, suggesting that these miRNAs may be used as biomarkers for detecting canine OA. This is the first report to comprehensively analyze the expression patterns of miRNAs in the synovial tissue of dogs with spontaneous OA.https://doi.org/10.1038/s41598-022-22362-2 |
| spellingShingle | Atsushi Yamazaki Yuma Tomo Hinano Eto Koji Tanegashima Kazuya Edamura A pilot study of microRNA assessment as a means to identify novel biomarkers of spontaneous osteoarthritis in dogs Scientific Reports |
| title | A pilot study of microRNA assessment as a means to identify novel biomarkers of spontaneous osteoarthritis in dogs |
| title_full | A pilot study of microRNA assessment as a means to identify novel biomarkers of spontaneous osteoarthritis in dogs |
| title_fullStr | A pilot study of microRNA assessment as a means to identify novel biomarkers of spontaneous osteoarthritis in dogs |
| title_full_unstemmed | A pilot study of microRNA assessment as a means to identify novel biomarkers of spontaneous osteoarthritis in dogs |
| title_short | A pilot study of microRNA assessment as a means to identify novel biomarkers of spontaneous osteoarthritis in dogs |
| title_sort | pilot study of microrna assessment as a means to identify novel biomarkers of spontaneous osteoarthritis in dogs |
| url | https://doi.org/10.1038/s41598-022-22362-2 |
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