Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol
Context Zuojin Pill (ZJP) has been used to treat gastrointestinal problems in China for hundreds of years.Objective To discover more potential active ingredients and evaluate the gastroprotective mechanisms of ZJP.Materials and methods An approach involving UPLC-Q-Orbitrap HRMS and serum pharmacoche...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2022.2098345 |
| _version_ | 1811343859742932992 |
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| author | Jiaying Zhang Yi Yin Qianqian Xu Xiaoqing Che Chen Yu Yan Ren Dongsheng Li Juanjuan Zhao |
| author_facet | Jiaying Zhang Yi Yin Qianqian Xu Xiaoqing Che Chen Yu Yan Ren Dongsheng Li Juanjuan Zhao |
| author_sort | Jiaying Zhang |
| collection | DOAJ |
| description | Context Zuojin Pill (ZJP) has been used to treat gastrointestinal problems in China for hundreds of years.Objective To discover more potential active ingredients and evaluate the gastroprotective mechanisms of ZJP.Materials and methods An approach involving UPLC-Q-Orbitrap HRMS and serum pharmacochemistry was established to screen the multiple chemical constituents of ZJP. Male Sprague-Dawley (SD) rats were divided into six groups: normal control, ulcer control, omeprazole (30 mg/kg), and three ZJP groups (1.0, 2.0 and 4.0 g/kg). After oral treatment with ZJP or omeprazole for 7 days, all groups except the normal control group were orally administered 5 mL/kg ethanol to induce gastric ulceration. Histopathological assessment of gastric tissue was performed by haematoxylin and eosin staining. Antioxidant parameters and inflammatory mediators were determined using ELISA Kit and immunohistochemical analysis.Results Ninety components were identified in ZJP. Among them, 23 prototypes were found in rat serum after oral administration of ZJP. The ulcer inhibition was over 90.0% for all the ZJP groups. Compared with the ulcer control rats, ZJP (4.0 g/kg) enhanced the antioxidant capacity of gastric tissue: superoxide dismutase (1.33-fold), catalase (2.61-fold), glutathione (2.14-fold), and reduced the malondialdehyde level (0.48-fold). Simultaneously, the ZJP meaningfully lowered the content of tumour necrosis factor-α (0.76-fold), interleukin-6 (0.66-fold), myeloperoxidase (0.21-fold), and nuclear factor kappa B (p65) (0.62-fold).Discussion and conclusions This study showed ZJP could mitigate ethanol-induced rat gastric ulcers, which might benefit from the synergistic actions of multiple ingredients. The findings could support the quality control and clinical trials of ZJP. |
| first_indexed | 2024-04-13T19:36:15Z |
| format | Article |
| id | doaj.art-1a73684293904226b9315b9e27956a1f |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-13T19:36:15Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-1a73684293904226b9315b9e27956a1f2022-12-22T02:33:00ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162022-12-016011417143510.1080/13880209.2022.2098345Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanolJiaying Zhang0Yi Yin1Qianqian Xu2Xiaoqing Che3Chen Yu4Yan Ren5Dongsheng Li6Juanjuan Zhao7School of Pharmacy, Binzhou Medical University, Yantai, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai, ChinaCollege of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai, ChinaContext Zuojin Pill (ZJP) has been used to treat gastrointestinal problems in China for hundreds of years.Objective To discover more potential active ingredients and evaluate the gastroprotective mechanisms of ZJP.Materials and methods An approach involving UPLC-Q-Orbitrap HRMS and serum pharmacochemistry was established to screen the multiple chemical constituents of ZJP. Male Sprague-Dawley (SD) rats were divided into six groups: normal control, ulcer control, omeprazole (30 mg/kg), and three ZJP groups (1.0, 2.0 and 4.0 g/kg). After oral treatment with ZJP or omeprazole for 7 days, all groups except the normal control group were orally administered 5 mL/kg ethanol to induce gastric ulceration. Histopathological assessment of gastric tissue was performed by haematoxylin and eosin staining. Antioxidant parameters and inflammatory mediators were determined using ELISA Kit and immunohistochemical analysis.Results Ninety components were identified in ZJP. Among them, 23 prototypes were found in rat serum after oral administration of ZJP. The ulcer inhibition was over 90.0% for all the ZJP groups. Compared with the ulcer control rats, ZJP (4.0 g/kg) enhanced the antioxidant capacity of gastric tissue: superoxide dismutase (1.33-fold), catalase (2.61-fold), glutathione (2.14-fold), and reduced the malondialdehyde level (0.48-fold). Simultaneously, the ZJP meaningfully lowered the content of tumour necrosis factor-α (0.76-fold), interleukin-6 (0.66-fold), myeloperoxidase (0.21-fold), and nuclear factor kappa B (p65) (0.62-fold).Discussion and conclusions This study showed ZJP could mitigate ethanol-induced rat gastric ulcers, which might benefit from the synergistic actions of multiple ingredients. The findings could support the quality control and clinical trials of ZJP.https://www.tandfonline.com/doi/10.1080/13880209.2022.2098345Multiple chemical constituentsantioxidationanti-inflammation |
| spellingShingle | Jiaying Zhang Yi Yin Qianqian Xu Xiaoqing Che Chen Yu Yan Ren Dongsheng Li Juanjuan Zhao Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol Pharmaceutical Biology Multiple chemical constituents antioxidation anti-inflammation |
| title | Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol |
| title_full | Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol |
| title_fullStr | Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol |
| title_full_unstemmed | Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol |
| title_short | Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol |
| title_sort | integrated serum pharmacochemistry and investigation of the anti gastric ulcer effect of zuojin pill in rats induced by ethanol |
| topic | Multiple chemical constituents antioxidation anti-inflammation |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2022.2098345 |
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